Seleksi dan Kemajuan Seleksi Karakter Komponen Hasil pada Persilangan Cabai Keriting dan Cabai Besar

Selection is very important activity in plant breeding program. The purpose of this study was to obtain genetic information, heritability and expected genetic advance, and to compare the genetic advance expectations with the selection advance. The research was conducted from September 2013 to November 2015 at Research Station of Leuwikopo, Dramaga, Bogor. Distribution of F2 population data in this study was broader than that of F3 populations. Broad sense heritability estimated by F3 populations showed in agreement to that estimated by F2 population. Character of fruit weight, fruit length, pedicel length and yield had positive value of selection advance appropriate to the genetic advance expectations in F2 populations. The value of heritability estimate and genetic advance were high indicating that the phenotypic variances were controlled by action of additive genes

Kemiripan dan Potensi Produksi Aksesi Pohpohan (Pilea Trinervia Wight.) dari Beberapa Daerah di Jawa Barat

Pohpohan (Pilea trinervia Wight.) is potential indigenous vegetable to be developed as a commercial vegetables. The objectives of this research were to observe similarity and production potential of pohpohan landraces from several areas in West Java. Result based on cluster analysis at nine similarity scale, thirteen exploration pohpohan landraces were grouped into three clusters. Clusters I consisted of Warung Loa, Tugu Selatan, Palasari, Langensari, Kayu Ambon, Lebak Muncang, Situsari, Sukalilah and Lebaksiuh. Clusters II consisted of Curug Rendeng, Argalingga and Linggarjati and clusters III was Bobojong. Result from further test using Duncan Multiple Range Test (DMRT), Warung Loa is a leading landrace because showed the best result on plant height, primary branches number, leaf width, yield per plot and plant productivity

Analisis Kemiripan 20 Aksesi Temu Ireng (Curcuma Aeruginosa Roxb.) Berdasarkan Warna Rimpang, Hasil Ekstrak, dan Kandungan Fitokimia

Curcuma aeruginosa Roxb., popularly known as “temu ireng”, is considered as a potential source of medicinal plant for pharmacological activities. However, varieties of C. aeruginosa are still limited in Indonesia so it needs more accessions for improvement and development of new varieties. Rhizome colors, phytochemical contents and extract yield from 20 promising lines of C. aeruginosa were investigated by qualitative method for rhizome colors and phytochemical contents, and maceration method using 70% ethanol for yield extract. Similarity analysis was used for cluster analysis based on rhizome colors, phytochemical contents and yield extract. Blue was the color characterization of rhizome C. aeruginosa. The extract yield for 20 promising lines of C. aeruginosa varied from 7.92 to 19.71%, with KN and BH promising lines having the lowest and highest value, respectively. All promising lines of C. aeruginosa contain saponin and triterpenoid. Based on similarity analysis, all promising lines could be divided into 3 clusters. Cluster I consisted of 14 promising lines i.e. WG, SH, KA, GD, BH, KP, NW, PW, MB, PR, PT, KN, MD, and PK. Cluster II consisted of 4 promising lines i.e. LC, CB, KL, and GK. Cluster III consisted of 2 promising lines i.e. KD and SG

Toleransi Galur Harapan Padi Sawah (Oryza Sativa L.) pada Persaingan dengan Gulma Echinochloa Crus-galli

Barnyard grass (Echinochloa crus-galli (L.) P. Beauv.) is a major weed competitor to rice production in Indonesia. In order to develop integrated weed management program, a research to select competitive rice lines to E. crus-galli was conducted in a green house of Indonesian Centre of Agricultural Biotechnology and Genetic Resource Research and Development Bogor. The research design was split plot with four replications, E. crus-galli was designed as the main plot (rice without E. crus-galli compared rice with four E. crus-galli per pot), and the sub-plots were 25 genotypes (23 lines, 1 tolerant variety and 1 sensitive variety). Level of tolerance was determined by the reduction percentage of grains weight, the number of productive tillers and dry matter weight. The results showed that rice competition with E. crus-galli reduced plant height, productive tiller numbers, filled spikelet numbers per panicle, dry matter weight and dry grain weight. Three lines, i.e., IR10L-155, IR10L-133 and BIO-R84-1 were classified as tolerant, 19 lines were moderate and 3 lines were sensitive to E. crus-galli competition

Aplikasi Bakteri Probiotik Pseudomonas Kelompok Fluorescens untuk Meningkatkan Produksi dan Mutu Benih Cabai

The use of high quality seed is one of the key factors to improve productivity. Probiotic bacteria has been used to increase plant growth and to control pathogens. The objective of the research was to evaluate methods of chili seed production that yielded high physiological and pathological quality using probiotic fluorescent bacteria Pseudomonas (P24). The bacteria was expected to function as plant growth promoting bacteria as well as capable of controling seedborne pathogen Colletotrichum acutatum causes antrachnose. The experiment was conducted during March until October 2014 in Seed Health Laboratory, Plant Bacteriology Laboratory, and Leuwikopo experimental garden, IPB. The experiment was arranged in a split plot randomized complete block design with three replications. The main plots were untreated and inoculation of C. acutatum. The subplot was six treatments of fluorescent Pseudomonas (P24) application. The results showed that matriconditioning and spraying of fluorescent Pseudomonas (P24) on nursery decreased disease incidence significantly. Matriconditioning and spraying of fluorescent Pseudomonas (P24) on nursery and flowering phase increased number of healthy fruit and total weight of seeds per plant significantly. These applications also increased seed physiological quality indicated by germination percentage (GP) 77.04%, growth rate (GR) 9.72% etmal-1, vigor index (VI) 29.74%, and seed health by suppresed C. acutatum infection up to 12.25%

Morfologi dan Pertumbuhan Planlet Hasil Induksi Poliploidi melalui Perlakuan Kolkisin pada Kuncup Bunga Anggrek Bulan (Phalaenopsis Amabilis (L.) Blume)

Induction of polyploid gametes is one of useful plant polyploidization methods. Some of its benefits are to obtain triploid and tetraploid progenies at the same time by cross and self pollination. Previous research showed that some morphological characters which could be the indications of polyploidy plantlets before the analysis of chromosome number. Colchicine treatment on flower bud of diploid Phalaenopsis amabilis was conducted to determine the effect of colchicine on flower bud development, plantlets morphology and growth, and potential of polyploidy induction based on plantlets morphology. Colchicine concentrations in the experiment were 0, 50, 500, 1,000, and 2,000 mg L-1, with three days duration of treatments with aluminium foil wraps on flower buds. The results showed that high colchicine concentrations (2,000 mg L-1) inhibited flowers blooming of treated flower buds. Based on morphological characters, plantlets were classified into normal and putative polyploid plantlets. Putative polyploid plantlets from colchicine with the concentration of 50, 500, and 1,000 mg L-1 were 71.2, 86.4, and 100.0% respectively