Understanding the Physiological and Molecular Mechanism of Persistent Organic Pollutant Uptake and Detoxification in Cucurbit Species (Zucchini and Squash)

Cucurbita pepo ssp pepo (zucchini) roots phytoextract significant amounts of persistent organic pollutants (POPs) from soil, followed by effective translocation to aboveground tissues. The closely related C. pepo ssp ovifera (squash) does not have this ability. In a DDE-contaminated field soil, zucchini roots and stems contained 3.6 and 6.6-fold greater contaminant than did squash tissues, respectively, and zucchini phytoextracted 12-times more DDE from soil than squash. In batch hydroponics, squash was significantly more sensitive to DDE (2−20 mg/L) exposure; 4 mg/L DDE significantly reduced squash biomass (14%) whereas for zucchini, biomass reductions were observed at 20 mg/L (20%). PCR select Suppression Subtraction Hybridization was used to identify differentially expressed genes in DDE treated zucchini relative to DDE treated squash or non-treated zucchini. After differential screening to eliminate false positives, unique cDNA clones were sequenced. Out of 40 shoot cDNA sequences, 34 cDNAs have homology to parts of phloem filament protein 1 (PP1). Out of 6 cDNAs from the root tissue, two cDNAs are similar to cytochrome P450 like proteins, and one cDNA matches a putative senescence associated protein. From the DDE exposed zucchini seedlings cDNA library, out of 22 differentially expressed genes, 14 cDNAs were found to have homology with genes involved in abiotic stresses, signaling, lipid metabolism, and photosynthesis. A large number of cDNA sequences were found to encode novel unknown proteins that may be involved in uncharacterized pathways of DDE metabolism in plants. A semiquantitative RT-PCR analysis of isolated genes confirmed up-regulation in response to DDE exposure

MOESM6 of Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic Camelina sativa expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase

Additional file 6: Table S6. GO classification and KEGG metabolic pathways for the DEGs down-regulated in DGAT1 lines at 16–21 DAF

MOESM11 of Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic Camelina sativa expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase

Additional file 11: Table S17. Metabolite contents in Camelina genotypes during seed development

MOESM12 of Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic Camelina sativa expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase

Additional file 12: Table S18. Relative metabolite content during seed development in Camelina genotype relative to WT as a control

Physiological and Molecular Response of <i>Arabidopsis thaliana</i> (L.) to Nanoparticle Cerium and Indium Oxide Exposure

The effects of cerium oxide (CeO₂) and indium oxide (In₂O₃) nanoparticles (NPs) exposure on <i>Arabidopsis thaliana</i> (L.) Heynh. were investigated. After inoculation in half strength MS medium amended with 0–2000 ppm CeO₂ and In₂O₃ NPs for 25 days, both physiological and molecular responses were evaluated. Exposure at 250 ppm CeO₂ NPs significantly increased plant biomass, but at 500–2000 ppm, plant growth was decreased by up to 85% in a dose-dependent fashion. At 1000 and 2000 ppm CeO₂ NPs, chlorophyll production was reduced by nearly 60% and 85%, respectively, and anthocyanin production was increased 3–5-fold. Malondialdehyde (MDA) production, a measure of lipid peroxidation, was unaffected by exposure to 250–500 ppm CeO₂ NPs, but at 1000 ppm, MDA formation was increased by 2.5-fold. Exposure to 25–2000 ppm In₂O₃ NPs had no effect on <i>A. thaliana</i> biomass and only minor effects (15%) on root elongation. Total chlorophyll and MDA production were unaffected by In₂O₃ NPs exposure. Molecular response to NP exposure as measured by qPCR showed that both types of elements altered the expression of genes central to the stress response such as the sulfur assimilation and glutathione (GSH) biosynthesis pathway, a series of genes known to be significant in the detoxification of metal toxicity in plants. Interestingly, In₂O₃ NPs exposure resulted in a 3.8–4.6-fold increase in glutathione synthase (GS) transcript production, whereas CeO₂ NPs yielded only a 2-fold increase. It seems likely that the significantly greater gene regulation response upon In₂O₃ NPs exposure was directly related to the decreased phytotoxicity relative to CeO₂ treatment. The use of NP rare earth oxide elements has increased dramatically, yet knowledge on fate and toxicity has lagged behind. To our knowledge, this is the first report evaluating both physiological and molecular plant response from exposure to these important nanoparticles

Reduced Silver Nanoparticle Phytotoxicity in Crambe abyssinica with Enhanced Glutathione Production by Overexpressing Bacterial γ‑Glutamylcysteine Synthase

Silver nanoparticles (Ag NPs) are widely used in consumer products, and their release has raised serious concerns about the risk of their exposure to the environment and to human health. However, biochemical mechanisms by which plants counteract NP toxicity are largely unknown. We have previously engineered Crambe abyssinica plants expressing the bacterial γ-glutamylecysteine synthase (γ-ECS) for enhancing glutathione (GSH) levels. In this study, we investigated if enhanced levels of GSH and its derivatives can protect plants from Ag NPs and AgNO₃ (Ag⁺ ions). Our results showed that transgenic lines, when exposed to Ag NPs and Ag⁺ ions, were significantly more tolerant, attaining a 28%–46% higher biomass and 34–49% more chlorophyll content, as well as maintaining 35–46% higher transpiration rates as compared to those of wild type (WT) plants. Transgenic γ-ECS lines showed 2–6-fold Ag accumulation in shoot tissue and slightly lower or no difference in root tissue relative to levels in WT plants. The levels of malondialdehyde (MDA) in γ-ECS lines were also 27.3–32.5% lower than those in WT Crambe. These results indicate that GSH and related peptides protect plants from Ag nanotoxicity. To our knowledge, this is the first direct report of Ag NP detoxification by GSH in transgenic plants, and these results will be highly useful in developing strategies to counteract the phytotoxicty of metal-based nanoparticles in crop plants