20 research outputs found
Learning Person-Specific Cognition From Facial Reactions for Automatic Personality Recognition
This article proposes to recognise the true (self-reported) personality traits from the target subject's cognition simulated from facial reactions. This approach builds on the following two findings in cognitive science: (i) human cognition partially determines expressed behaviour and is directly linked to true personality traits; and (ii) in dyadic interactions, individuals’ nonverbal behaviours are influenced by their conversational partner's behaviours. In this context, we hypothesise that during a dyadic interaction, a target subject's facial reactions are driven by two main factors: their internal (person-specific) cognitive process, and the externalised nonverbal behaviours of their conversational partner. Consequently, we propose to represent the target subject's (defined as the listener) person-specific cognition in the form of a person-specific CNN architecture that has unique architectural parameters and depth, which takes audio-visual non-verbal cues displayed by the conversational partner (defined as the speaker) as input, and is able to reproduce the target subject's facial reactions. Each person-specific CNN is explored by the Neural Architecture Search (NAS) and a novel adaptive loss function, which is then represented as a graph representation for recognising the target subject's true personality. Experimental results not only show that the produced graph representations are well associated with target subjects’ personality traits in both human-human and human-machine interaction scenarios, and outperform the existing approaches with significant advantages, but also demonstrate that the proposed novel strategies help in learning more reliable personality representations.</p
Exogenous PABA improves thermotolerance of strain <i>8213</i>.
<p>(A) Strains <i>02</i> and <i>8213</i> mycelium culture were supplied with or without 5 mg/L PABA, followed by high temperature treatment at 33°C for 48 hours, the images were taken at the indicated time post-heat stress. The control groups were cultured under regular temperature of 23°C. (B) Dose-dependent protection by PABA of mycelium intactness under heat stress at 33°C for 48 hours. Strain <i>02</i> and strain <i>8213</i> were treated with different concentrations of PABA followed by high-temperature treatment at 33°C for 48 h, and the percentage of intact mycelia was counted. The intact mycelia percentage was calculated as compared to the control group (23°C). Three independent biological replicates were performed. Data are expressed as average ± SEM. Unpaired t-tests were performed between samples with and without PABA addition within each strain, respectively, ns: P>0.05, *: P<0.05, **: P<0.01.</p
PABA mediates the accumulation of defense-related proteins in <i>02</i>, <i>8213</i> and <i>TB-2</i> under heat stress.
<p>(A) Defense-related proteins HSPs and Chitinase accumulated more in thermotolerant strain <i>02</i> than in thermo-sensitive strain <i>8213</i> under heat stress (33°C). (B) Exogenous PABA (1 mM) application increases accumulation of HSPs and Chitinase in <i>8213</i> under heat stress (33°C). (C) PABA synthase inhibitor Sulfanilamide (0.1 mM) decreases accumulation of HSPs and Chitinase in <i>02</i> under heat stress (33°C). D) <i>Pabs</i>-overexpressing transgenic line <i>TB-2</i> accumulates more HSPs and Chitinase than the parent strain <i>8213</i> under heat stress (33°C). Three independent biological replicates were performed for each sample.</p
Identification and classification of differentially expressed proteins between <i>02</i> and <i>8213</i>.
<p>(A) Venn diagram showing 20 up-regulated and 5 down-regulated proteins among 48 identified proteins comparing <i>02</i>-HS with <i>02</i>-NS. Functional classification of those 25 heat stress induced proteins is shown on the bottom. (B) Heat-map is shown to indicate relative protein abundance among the 4 samples. The abundance of each protein spot in <i>02</i>-NS was given a reference value of 1.0, the abundance of that spot from <i>02</i>-HS, <i>8213</i>-NS and <i>8213</i>-HS were transformed into relative value. The different colors correspond to the values of protein level changes as indicated by the bar at the bottom of the heat map. Venn diagrams present the two-step filtering process leading to 4 candidates to further select out the more important candidate proteins for functional studies (see text). The four asterisks mark the proteins that are upregulated by heat-stress in thermotolerant strain <i>02</i>, not upregulated by heat stress in thermo-sensitive strain <i>8213,</i> and expressed at lower levels in thermo-sensitive strain <i>8213</i> than in the thermotolerant strain <i>02</i> with or without heat-stress. <i>02</i>-NS: <i>02</i> non-stressed (23°C/24 h); <i>02</i>-HS: <i>02</i> heat-stressed (33°C/24 h); <i>8213</i>-NS: <i>8213</i> non-stressed (23°C/24 h); <i>8213</i>-HS: strain <i>8213</i> heat-stressed (33°C/24 h).</p
PABA reduces H<sub>2</sub>O<sub>2</sub> accumulation in heat stressed strains <i>02</i> and <i>8213</i>.
<p>(A) Time course of accumulation of H<sub>2</sub>O<sub>2</sub> in the mycelia of strains <i>02</i>, <i>8213</i>, <i>TB-2</i> and <i>TB-3</i> under heat stress (33°C). Strains <i>02</i>, <i>TB-2</i> and <i>TB-3</i>, which produce more PABA content than strain <i>8213</i>, have less H<sub>2</sub>O<sub>2</sub> accumulation under parallel conditions. (B) Effects of PABA and PABA synthase inhibitor sulfanilamide on H<sub>2</sub>O<sub>2</sub> accumulation. Strain <i>02</i> and strain <i>8213</i> were cultured on PDA medium with 1 mM PABA or 0.1 mM sulfanilamide for 6 days, then were subjected to high temperature treatment (33°C) for 48 hours as indicated, followed by H<sub>2</sub>O<sub>2</sub> content measurement. Three independent biological replicates were performed for each analysis. Data are expressed as average ± SEM. Unpaired t-tests were performed between control sample and sulfanilamide treated sample or PABA treated sample within each strain, ns: P>0.05, *: P<0.05, **: P<0.01.</p
Mushroom strain <i>02</i> is more thermotolerant than strain <i>8213</i>.
<p>Strains <i>02</i> and <i>8213</i> had been cultivated under the same regular condition until about 1 week before fruit body formation, each was then divided into two groups. One half was maintained at 18°C (control group); the other half was maintained at 30°C (high-temperature group) for 7 days. At Day 7, the fruit bodies were photographed (A), and the pileus diameter and biomass weight were recorded (B). Three independent replicates were performed. Data are expressed as average ± SEM. Unpaired t-tests were performed, ns: P>0.05, **: P<0.01.</p
PABA synthase inhibitor sulfanilamide inhibits PABA production and impairs mycelium intactness.
<p>Mushrooms were treated with increasing concentrations of sulfanilamide for 6 days, and then subjected to heat stress (33°C) for 48 hours. PABA content (A) and the percentage of intact mycelium (B) were determined after heat stress treatment.</p
Transgenic overexpression of PABA synthase improves thermotolerance of strain <i>8213</i>.
<p>(A) Relative mRNA level of <i>Pabs</i> gene of strains <i>02</i>, <i>8213</i> and two <i>Pabs</i>-overexpressing transgenic strains <i>TB-2</i> and <i>TB-3</i> (derived from <i>8213</i>) under normal temperature (23°C) and heat stress (33°C). The mRNA of corresponding samples was extracted and analyzed after 24 hours of treatment. (B) The PABA content of strains <i>02</i>, <i>8213</i> and <i>TB-2</i> and <i>TB-3</i> under normal temperature (23°C) and heat stress (33°C) for 3 days. The PABA content of corresponding samples was extracted and measured after 3 days of treatment. (C) The mycelia growth of Strains <i>02</i>, <i>8213</i> and <i>TB-2</i> and <i>TB-3</i> under normal temperature (23°C) and heat stress (33°C). Mycelia cultures were photographed after 2 weeks of treatment. (D) The mycelia elongation of strains <i>02</i>, <i>8213</i> and <i>TB-2</i> and <i>TB-3</i> under normal temperature (23°C) and heat stress (33°C). The mycelia length is measured after 14 and 21 days of treatment. Three independent biological replicates were performed for each analysis. Data are expressed as average ± SEM. Unpaired t-tests were performed between strain <i>8213</i> and all other strains as indicated within each treatment condition, ns: P>0.05, *: P<0.05, **: P<0.01.</p
Exogenous PABA improves thermotolerance of strain <i>8213</i>.
<p>(A) Strains <i>02</i> and <i>8213</i> mycelium culture were supplied with or without 5 mg/L PABA, followed by high temperature treatment at 33°C for 48 hours, the images were taken at the indicated time post-heat stress. The control groups were cultured under regular temperature of 23°C. (B) Dose-dependent protection by PABA of mycelium intactness under heat stress at 33°C for 48 hours. Strain <i>02</i> and strain <i>8213</i> were treated with different concentrations of PABA followed by high-temperature treatment at 33°C for 48 h, and the percentage of intact mycelia was counted. The intact mycelia percentage was calculated as compared to the control group (23°C). Three independent biological replicates were performed. Data are expressed as average ± SEM. Unpaired t-tests were performed between samples with and without PABA addition within each strain, respectively, ns: P>0.05, *: P<0.05, **: P<0.01.</p
2-D electrophoresis of protein extracts from <i>02</i> and <i>8213</i> with or without heat stress (33°C/24 h).
<p>(A) Representative 2-DE gels of mushrooms, identifying 73 proteins with a greater than 2-fold difference after high-temperature treatment (p<0.05). Molecular weight (MW) in kilodaltons and pI of proteins are indicated on the left and top of the gel, respectively. (B) Close-up view of some differentially expressed proteins spots. Three independent replicates were performed. <i>02</i>-NS: <i>02</i> non-stressed (23°C/24 h); <i>02</i>-HS: <i>02</i> heat-stressed (33°C/24 h); <i>8213</i>-NS: <i>8213</i> non-stressed (23°C/24 h); <i>8213</i>-HS: strain <i>8213</i> heat-stressed (33°C/24 h).</p