A Metal- and Azide-Free Multicomponent Assembly toward Regioselective Construction of 1,5-Disubstituted 1,2,3-Triazoles

The construction of 1,5-disubstituted 1,2,3-triazoles has been effected through the cascade dual C–N bond formation, N–N bond formation and an acyl migration-based C–C bond formation via the three-component reactions of enaminones, tosylhydrazine and primary amines. This metal- and azide-free, regioselective synthetic method proceeds in the presence of only molecular iodine

Base-Promoted Synthesis of <i>N</i>‑Substituted 1,2,3-Triazoles via Enaminone–Azide Cycloaddition Involving Regitz Diazo Transfer

The domino reactions between NH-based secondary enaminones and tosyl azide have been developed for the synthesis of various <i>N</i>-substituted 1,2,3-triazoles by employing <i>t</i>-BuONa as the base promoter. Through a key Regitz diazo-transfer process with tosyl azide, the reactions proceed efficiently at room temperature with good substrate tolerance

Domino Reactions Involving the Branched C–N and CC Cleavage of Enaminones Toward Pyridines Synthesis

The copper-catalyzed cascade reactions of enaminones and ammonium chloride have led to the unprecedented synthesis of 4-unsubstituted pyridines of both symmetrical and unsymmetrical structures. Under the aerobic copper-catalyzed conditions, the branched transformations of enaminones with C–N and CC bond cleavage provide the C2-C3/C5-C6 and C4 building blocks to construct the pyridine ring, respectively. The CC cleavage that provides the C4 atom in the pyridine product is the first example showing the reactivity of an enaminone as the donor of one carbon synthon

Base-Promoted Synthesis of <i>N</i>‑Substituted 1,2,3-Triazoles via Enaminone–Azide Cycloaddition Involving Regitz Diazo Transfer

The domino reactions between NH-based secondary enaminones and tosyl azide have been developed for the synthesis of various <i>N</i>-substituted 1,2,3-triazoles by employing <i>t</i>-BuONa as the base promoter. Through a key Regitz diazo-transfer process with tosyl azide, the reactions proceed efficiently at room temperature with good substrate tolerance

Domino Reactions Involving the Branched C–N and CC Cleavage of Enaminones Toward Pyridines Synthesis

The copper-catalyzed cascade reactions of enaminones and ammonium chloride have led to the unprecedented synthesis of 4-unsubstituted pyridines of both symmetrical and unsymmetrical structures. Under the aerobic copper-catalyzed conditions, the branched transformations of enaminones with C–N and CC bond cleavage provide the C2-C3/C5-C6 and C4 building blocks to construct the pyridine ring, respectively. The CC cleavage that provides the C4 atom in the pyridine product is the first example showing the reactivity of an enaminone as the donor of one carbon synthon

A new chromene from the fruiting bodies of <i>Chroogomphus rutilus</i>

<div><p>A new chromene, acetic acid 2<i>R</i>-(4,8-dimethylnona-3,7-dienyl)-8-hydroxy-2-methyl-2<i>H</i>-chromen-6-yl ester (<b>1</b>), was isolated from the fruiting bodies of <i>Chroogomphus rutilus</i>, together with six known compounds (<b>2</b>–<b>7</b>). The structures of these compounds were identified based on 1D and 2D NMR, including ¹H–¹H COSY, HMQC and HMBC spectroscopic methods. Of these seven compounds, <b>2</b> and <b>3</b> showed cytotoxicity against HSC-T6, SK-Hep1 and A549 cell lines.</p></div

Two new diarylheptanoids isolated from the roots of <i>Juglans mandshurica</i>

<div><p>Two new diarylheptanoids, ( − )-<i>threo</i>-3′,4″-epoxy-1-(4-hydroxyphenyl)-7-(3-methoxyphenyl)heptan-2,3-diol (<b>1</b>) and (1α,3β,5α,6α)-1,5-epoxy-3,6-dihydroxy-1,7-bis(3-methoxy-4-hydroxy-phenyl)-heptane (<b>2</b>), along with one known diarylheptanoid, rhoiptelol B (<b>3</b>), were isolated from the roots of <i>Juglans mandshurica</i>. The structures of compounds <b>1</b> and <b>2</b> were identified based on HR-ESI-MS, 1D and 2D NMR including ¹H–¹H COSY, HMQC, HMBC and NOESY spectroscopic methods.</p></div

Additional file 1: Figure S1. of Knockdown of SOX2OT inhibits the malignant biological behaviors of glioblastoma stem cells via up-regulating the expression of miR-194-5p and miR-122

The expression of SOX2OT after cells transfection with four short-harpin plasmids of SOX2OT (SOX2OT-Homo-190, SOX2OT-Homo-456, SOX2OT-Homo-641, SOX2OT-Homo-847). Figure S2. The expression and effects of SOX2OT in GSC-GBM. Figure S3. MiR-194-5p and miR-122 exerted tumor-suppressive functions in GSC-GBM. Figure S4. SOX3 and TDGF-1 played oncogenic roles in GSC-GBM. Figure S5. SOX3 mediated tumor-suppressive effects of miR-194-5p and miR-122. (DOCX 5068 kb