The stability of M7E_No.2 in CS- <i>Th. elongatum</i> disomic substitution lines.

<p>M: Marker (DL2000); 1: DS1E (1A); 2: DS1E (1B); 3: DS1E (1D); 4: DS2E (2A); 5: DS2E (2B); 6: DS2E(2D); 7: DS3E(3A); 8: DS3E(3B); 9: DS3E(3D); 10: DS4E(4A); 11: DS4E(4B); 12: DS4E(4D); 13: DS5E(5B); 14: DS5E(5D); 15: DS6E(6A); 16: DS6E(6D); 17: DS7E(7A); 18: DS7E(7B); 19: DS7E(7D); 20: CS; 21: <i>Th. elongatum</i> (2<i>n</i> = 2<i>X</i>).</p

The 7E chromosome-specific molecular markers and PCR primers of <i>Thinopyrum elongatum.</i>

<p>A list of the names of the specific markers is shown, where M7E_No.1 stands for the first (No.1) molecular marker (M) of the <i>Thinopyrum elongatum</i> 7E-chromosome (7E). A list of the name of the specific primers is shown, where P7E_No.1 stands for the first pair (No.1) of primers (P) of the <i>Thinopyrum elongatum</i> 7E-chromosome (7E). Additionally, the name of the original fragments is listed, where SLAF119658 stands for specific (S) length (L) amplified (A) fragment (F), and its number is 119658.</p

The stability of M7E_No.2 in F₁ and F₂ of orthogonal (A) and reciprocal (B) cross offspring of Y16 and DS7E (7A).

<p>M:Marker (DL2000); 1: YD-F₁ (A) or DY-F₁ (B); 2–19: YD-F₂ (A) or DY-F₂ (B); 20: Y16; 21: DS7E(7A).</p

The experimental materials used in this study.

<p>The experimental materials used in this study.</p

The stability of M7E_No.2 in other wheat, amphidiploid and polyploid <i>Th. Elongatum</i>.

<p>M: Marker (DL2000); 1: LD; 2: Y10; 3: Y14; 4: Y16; 5: Y18; 6: Y158; 7: N13; 8: An 8455; 9: Su 3; 10∶8801; 11: <i>Th. elongatum</i> (2<i>n</i> = 4<i>X</i>); 12: <i>Th. elongatum</i> (2<i>n</i> = 10<i>X</i>, PI179162); 13: <i>Th. elongatum</i> (2<i>n</i> = 10<i>X</i>, PI204383); 14: CS; 15: <i>Th. elongatum</i> (2<i>n</i> = 2<i>X</i>).</p

The PCR amplification of M7E_No.1 (A), M7E_No.2 (B) and M7E_No.9 (C) in CS- <i>Thelongatum</i> disomic addition and 7E telodisomic addition lines.

<p>M: Marker (DL2000); 1–7: DA1E-DA7E; 8: CS; 9: <i>Th. elongatum</i> (2<i>n</i> = 2<i>X</i>); 10: DA7ES; 11: DA7EL.</p

The DNA sequences and length of the <i>Thinopyrum elongatum</i> 7E chromosome-specific molecular marker M7E_No.2.

<p>The DNA sequences and length of the <i>Thinopyrum elongatum</i> 7E chromosome-specific molecular marker M7E_No.2.</p

A new criteria for acute on preexisting kidney dysfunction in critically ill patients

Critically ill patients with preexisting kidney dysfunction (PKD) are at high risk for acute kidney injury (AKI). Nevertheless, there is no criteria for screening and classifying AKI in patients with PKD. In this study, after assessing relationship between the change in SCr from baseline and in-hospital mortality, a new criteria, named APKD, for identifying AKI in PKD was proposed. APKD defined AKI in critically ill patients with PKD as an absolute increase of ≥ 0.2 mg/dL in SCr within 48 h or an increase in SCr ≥ 1.1 times over baseline within 7 d. APKD detected more AKI among PKD patients compared with the other criteria. Additionally, the AKI patients identified by APKD but missed by the other criteria had higher mortality than those without AKI. APKD shows higher sensitivities than KDIGO criteria in predicating in-hospital mortality. APKD, but not the KDIGO, is effective for staging the severity of AKI in patients with PKD. In conclusion, APKD is more effective in screening and classifying AKI in critically ill patients with PKD compared with the earlier criteria, and it may helpful in guiding clinical treatment and predicting prognosis.</p