Detection and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter

The ribosome is the quintessential antibacterial drug target, with many structurally and mechanistically distinct classes of antibacterial agents acting by inhibiting ribosome function. Detecting and quantifying ribosome inhibition by small molecules and investigating their binding modes and mechanisms of action are critical to antibacterial drug discovery and development efforts. To develop a ribosome inhibition assay that is operationally simple, yet provides direct information on the drug target and the mechanism of action, we have developed engineered <i>E. coli</i> strains harboring an orthogonal ribosome-controlled green fluorescent protein (GFP) reporter that produce fluorescent signal when the orthogonal ribosome is inhibited. As a proof of concept, we demonstrate that these strains, when coexpressing homogeneous populations of aminoglycoside resistant ribosomes, act as sensitive and quantitative detectors of ribosome inhibition by a set of 12 structurally diverse aminoglycoside antibiotics. We suggest that this strategy can be extended to quantifying ribosome inhibition by other drug classes

Image_3_v1_A modified survival model for patients with esophageal squamous cell carcinoma based on lymph nodes: A study based on SEER database and external validation.tif

BackgroundThe counts of examined lymph nodes (ELNs) in predicting the prognosis of patients with esophageal squamous cell carcinoma (ESCC) is a controversial issue. We conducted a retrospective study to develop an ELNs-based model to individualize ESCC prognosis.MethodsPatients with ESCC from the SEER database and our center were strictly screened. The optimal threshold value was determine by the X-tile software. A prognostic model for ESCC patients was developed and validated with R. The model’s efficacy was evaluated by C-index, ROC curve, and decision curve analysis (DCA).Results3,629 cases and 286 cases were screened from the SEER database and our center, respectively. The optimal cut-off value of ELNs was 10. Based on this, we constructed a model with a favorable C-index (training group: 0.708; external group 1: 0.687; external group 2: 0.652). The model performance evaluated with ROC curve is still reliable among the groups. 1-year AUC for nomogram in three groups (i.e., 0.753, 0.761, and 0.686) were superior to that of the TNM stage (P ConclusionMore than 10 ELNs are helpful to evaluate the survival of ESCC patients. Based on this, an improved model for predicting the prognosis of ESCC patients was proposed.</p

Image_2_v1_A modified survival model for patients with esophageal squamous cell carcinoma based on lymph nodes: A study based on SEER database and external validation.tif

BackgroundThe counts of examined lymph nodes (ELNs) in predicting the prognosis of patients with esophageal squamous cell carcinoma (ESCC) is a controversial issue. We conducted a retrospective study to develop an ELNs-based model to individualize ESCC prognosis.MethodsPatients with ESCC from the SEER database and our center were strictly screened. The optimal threshold value was determine by the X-tile software. A prognostic model for ESCC patients was developed and validated with R. The model’s efficacy was evaluated by C-index, ROC curve, and decision curve analysis (DCA).Results3,629 cases and 286 cases were screened from the SEER database and our center, respectively. The optimal cut-off value of ELNs was 10. Based on this, we constructed a model with a favorable C-index (training group: 0.708; external group 1: 0.687; external group 2: 0.652). The model performance evaluated with ROC curve is still reliable among the groups. 1-year AUC for nomogram in three groups (i.e., 0.753, 0.761, and 0.686) were superior to that of the TNM stage (P ConclusionMore than 10 ELNs are helpful to evaluate the survival of ESCC patients. Based on this, an improved model for predicting the prognosis of ESCC patients was proposed.</p

Image_1_v1_A modified survival model for patients with esophageal squamous cell carcinoma based on lymph nodes: A study based on SEER database and external validation.tif

BackgroundThe counts of examined lymph nodes (ELNs) in predicting the prognosis of patients with esophageal squamous cell carcinoma (ESCC) is a controversial issue. We conducted a retrospective study to develop an ELNs-based model to individualize ESCC prognosis.MethodsPatients with ESCC from the SEER database and our center were strictly screened. The optimal threshold value was determine by the X-tile software. A prognostic model for ESCC patients was developed and validated with R. The model’s efficacy was evaluated by C-index, ROC curve, and decision curve analysis (DCA).Results3,629 cases and 286 cases were screened from the SEER database and our center, respectively. The optimal cut-off value of ELNs was 10. Based on this, we constructed a model with a favorable C-index (training group: 0.708; external group 1: 0.687; external group 2: 0.652). The model performance evaluated with ROC curve is still reliable among the groups. 1-year AUC for nomogram in three groups (i.e., 0.753, 0.761, and 0.686) were superior to that of the TNM stage (P ConclusionMore than 10 ELNs are helpful to evaluate the survival of ESCC patients. Based on this, an improved model for predicting the prognosis of ESCC patients was proposed.</p

Solvent-Controlled Synthesis of Zn–Co–S@Ni₁₂P₅ Arrays with Ultra-High Specific Capacitance for Hybrid Supercapacitor

The solvents used in the preparation process have a substantial influence on the structure and morphology of electrode materials, which in turn can impact the electrochemical performance for the supercapacitor. In this work, nickel foam substrate was subjected to a low-temperature phosphating reaction and then transition metal sulfides were controlled grown on the surface of Ni12P5/NF with different morphology by tuning the ratios of reaction solvents (H2O: C2H5OH). The optimized Zn–Co–S@Ni12P5/NF-1 nanosheet has an ultrahigh specific capacitance (10.7 F cm–2 at 1 mA cm–2), which originates from both the special nanosheet structure with an abundance of active sites, as well as the synergistic interaction between metal sulfides and phosphides. The assembled hybrid supercapacitor (HSC) device (Zn–Co–S@Ni12P5/NF-1//AC) has a high storage capacity (40.11 Wh/kg at 803.09 W/kg) and maintains 85.2% retention rate after 5,000 cycles. Moreover, the all-solid hybrid supercapacitors effectively lit up 9 LEDs for 2 min, which means it holds significant promise for practical energy storage applications

Diverse Sesquiterpenoids and Polyacetylenes from <i>Atractylodes lancea</i> and Their Anti-Osteoclastogenesis Activity

Twenty-two sesquiterpenoids (1–22) and 11 polyacetylenes (23–33) were obtained from the rhizomes of Atractylodes lancea. Among them, 11 compounds (1–5, 11, 12, 23, 24, 30, and 31) are new. The scaffolds represented by the isolates of sesquiterpenoids were found to be varied and included two rare rearranged spirovetivane sesquiterpenoids with a spiro [4,4] skeleton, eight spirovetivanes, three guaianes, eight eudesmanes, and one eremophilane. Their planar structures and relative configurations were elucidated by UV, IR, 1D and 2D NMR, and HRESIMS data analysis. The absolute configurations of the new sesquiterpenoids were determined using X-ray diffraction analysis and by comparison of the calculated and experimental electronic circular dichroism and optical rotation data, as well as chemical transformations. All the isolated compounds (1–33) were evaluated for their activity against RANKL-induced osteoclastogenesis in bone marrow macrophages. Two polyacetylene-type compounds, 25 and 32, showed potent activity with IC50 values of 1.3 and 0.64 μM, respectively. Rearranged spirovetivane sesquiterpenoids with a spiro [4,4] skeleton are reported herein from the genus Atractylodes for the first time. Polyacetylenes were demonstrated as the main active constituents of A. lancea with osteoclastogenesis inhibitory activity

Diverse Sesquiterpenoids and Polyacetylenes from <i>Atractylodes lancea</i> and Their Anti-Osteoclastogenesis Activity

Twenty-two sesquiterpenoids (1–22) and 11 polyacetylenes (23–33) were obtained from the rhizomes of Atractylodes lancea. Among them, 11 compounds (1–5, 11, 12, 23, 24, 30, and 31) are new. The scaffolds represented by the isolates of sesquiterpenoids were found to be varied and included two rare rearranged spirovetivane sesquiterpenoids with a spiro [4,4] skeleton, eight spirovetivanes, three guaianes, eight eudesmanes, and one eremophilane. Their planar structures and relative configurations were elucidated by UV, IR, 1D and 2D NMR, and HRESIMS data analysis. The absolute configurations of the new sesquiterpenoids were determined using X-ray diffraction analysis and by comparison of the calculated and experimental electronic circular dichroism and optical rotation data, as well as chemical transformations. All the isolated compounds (1–33) were evaluated for their activity against RANKL-induced osteoclastogenesis in bone marrow macrophages. Two polyacetylene-type compounds, 25 and 32, showed potent activity with IC50 values of 1.3 and 0.64 μM, respectively. Rearranged spirovetivane sesquiterpenoids with a spiro [4,4] skeleton are reported herein from the genus Atractylodes for the first time. Polyacetylenes were demonstrated as the main active constituents of A. lancea with osteoclastogenesis inhibitory activity

Silicon-Nanomembrane-Based Broadband Synaptic Phototransistors for Neuromorphic Vision

Neuromorphic vision has been attracting much attention due to its advantages over conventional machine vision (e.g., lower data redundancy and lower power consumption). Here we develop synaptic phototransistors based on the silicon nanomembrane (Si NM), which are coupled with lead sulfide quantum dots (PbS QDs) and poly(3-hexyl­thiophene) (P3HT) to form a heterostructure with distinct photogating. Synaptic phototransistors with optical stimulation have outstanding synaptic functionalities ranging from ultraviolet (UV) to near-infrared (NIR). The broadband synaptic functionalities enable an array of synaptic phototransistors to achieve the perception of brightness and color. In addition, an array of synaptic phototransistors is capable of simultaneous sensing, processing, and memory, which well mimics human vision

Diverse Sesquiterpenoids and Polyacetylenes from <i>Atractylodes lancea</i> and Their Anti-Osteoclastogenesis Activity

Twenty-two sesquiterpenoids (1–22) and 11 polyacetylenes (23–33) were obtained from the rhizomes of Atractylodes lancea. Among them, 11 compounds (1–5, 11, 12, 23, 24, 30, and 31) are new. The scaffolds represented by the isolates of sesquiterpenoids were found to be varied and included two rare rearranged spirovetivane sesquiterpenoids with a spiro [4,4] skeleton, eight spirovetivanes, three guaianes, eight eudesmanes, and one eremophilane. Their planar structures and relative configurations were elucidated by UV, IR, 1D and 2D NMR, and HRESIMS data analysis. The absolute configurations of the new sesquiterpenoids were determined using X-ray diffraction analysis and by comparison of the calculated and experimental electronic circular dichroism and optical rotation data, as well as chemical transformations. All the isolated compounds (1–33) were evaluated for their activity against RANKL-induced osteoclastogenesis in bone marrow macrophages. Two polyacetylene-type compounds, 25 and 32, showed potent activity with IC50 values of 1.3 and 0.64 μM, respectively. Rearranged spirovetivane sesquiterpenoids with a spiro [4,4] skeleton are reported herein from the genus Atractylodes for the first time. Polyacetylenes were demonstrated as the main active constituents of A. lancea with osteoclastogenesis inhibitory activity

DataSheet1_Stability and Thermoelasticity of Diaspore by Synchrotron X-ray Diffraction and Raman Spectroscopy.docx

The thermoelasticity and stability of diaspore (α-AlOOH, Al1.002Fe0.003OOH) were investigated in this study by in situ synchronous X-ray diffraction (XRD) and Raman spectroscopy methods at high pressure and high temperature conditions. The results indicate that diaspore is stable within the pressure and temperature (P-T) region examined in this study. With increasing pressure, the Raman peaks move toward the high wave number direction, the intensity of the Raman peaks increases, and the vibration mode of diaspore changes linearly. Pressure-volume data from in situ high-pressure XRD experiments were fitted by the third-order Birch-Murnaghan equation of state (EoS) with the zero-pressure unit-cell volume V0 = 118.15 (4) Å3, the zero-pressure bulk modulus KV0 = 153 (2) GPa, and its pressure derivative K'V0 = 2.4 (3). When K'V0 was fixed at 4, the obtained KV0 = 143 (1) GPa. The axial compressional behavior of diaspore was also fitted with a linearized third-order Birch-Murnaghan EoS, showing slight compression anisotropy with Ka0 = 137 (5) GPa, Kb0 = 169 (7) GPa and Kc0 = 178 (6) GPa. In addition, the temperature-volume data from in situ high-temperature XRD experiments were fitted by Fei’s thermal equation with the thermal expansion coefficients αV = 2.7 (2) × 10–5 K−1, αa = 1.13 (9) × 10–5 K−1, αb = 0.77 (5) × 10–5 K−1, and αc = 0.85 (9) × 10–5 K−1 for diaspore, which shows that diaspore exhibits slightly anisotropic thermal expansion. Furthermore, in situ synchrotron-based single-crystal XRD under simultaneously high P-T conditions indicates that the P-T stability of diaspore is up to ∼10.9 GPa and 700 K. Combined with previous results, we infer that diaspore can be subducted to ∼390 km under cold subduction conditions based on existing experimental data and is a good candidate for transporting water to the deep Earth.</p