Concentration (pg/mg) of cholesterol metabolites in various tissues after incubation with normal cholesterol (C) and labeled Cholesterol-[4-¹³C] (C-[4-¹³C]).

<p>Retention time (R_t), foregut (FG), midgut (MG) and hindgut (HG).</p><p>^○ Identified by comparison with authentic samples.</p><p>^◊ Identification by search in the spectra database and through derivatization.</p><p>✘ denotes not detected.</p><p>Concentration (pg/mg) of cholesterol metabolites in various tissues after incubation with normal cholesterol (C) and labeled Cholesterol-[4-¹³C] (C-[4-¹³C]).</p

Representative total ion chromatogram of the oral secretion, foregut and midgut of the desert locust after feeding on wheat seedlings.

<p>Numbers 1–13 indicate sterols identified as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127171#pone.0127171.t001" target="_blank">Table 1</a>; IS, internal standard.</p

Representative total ion chromatogram of the foregut and midgut of the desert locust after incubation with cholesterol-[4-¹³C] with an expanded view of the identified cholesterol metabolites (retention time 35–50 min)].

<p>Representative total ion chromatogram of the foregut and midgut of the desert locust after incubation with cholesterol-[4-¹³C] with an expanded view of the identified cholesterol metabolites (retention time 35–50 min)].</p

Concentrations (pg/g) of sterols in 5^th instars desert locusts fed on wheat seedlings.

<p>Retention time (R_t), wheat seedlings (W), oral secretion (OS), foregut (FG), midgut (MG), hindgut (HG), frass (F), hemolymph (H) and fat body (FB).</p><p>Concentrations of sterol bearing the same letter in various tissues and secretions (across the column) are not significantly different (P≤0.05, Tukey’s, HSD test).</p><p>^○ Identified by comparison with authentic samples.</p><p>^◊Identification by library data and through derivatization.</p><p>✘ denotes not detected.</p><p>Concentrations (pg/g) of sterols in 5^th instars desert locusts fed on wheat seedlings.</p

Structures of sterols.

<p>Δ indicates double bond and respective positions.</p

Total ion chromatogram of wheat seedlings extract.

<p>Numbers indicate eight of the thirteen sterols identified as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127171#pone.0127171.t001" target="_blank">Table 1</a>; IS, internal standard.</p