Media 1: In vivo femtosecond endosurgery: an intestinal epithelial regeneration-after-injury model

Originally published in Optics Express on 16 December 2013 (oe-21-25-30842

Supplement 1: Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies

Supplementary figures and text Originally published in Optica on 20 February 2017 (optica-4-2-222

Controlled Detachment of Chemically Glued Cells

We demonstrate a chemically detachable cell–glue system based on linkers containing disulfide bonds as well as functional groups for metabolic glycoengineering and bioorthogonal click chemistry. Azide groups are generated on the cell surface by metabolic glycoengineering, and they are further modified into tetrazine (Tz) or trans-cyclooctene (TCO) using rationally designed cross-linkers. When the Tz-modified and TCO-modified cells are mixed together, cell gluing between these two cell groups is established by Tz-TCO click chemistry. This artificial cell–cell adhesion can be broken by the administration of glutathione (5 mM), which triggers the degradation of disulfide bonds. Both the gluing and detachment processes are rapid (<10 min) and minimally cytotoxic

Sub-micron single-particle perovskite plasmonic nanolasers at room temperature

Plasmonic nanolasers have received a substantial interest for their promising applications in integrated photonics, optical sensing, and biomedical imaging. To date, a room-temperature plasmonic nanolaser, submicron in all dimensions, remains elusive in the visible regime due to high metallic losses. Here, we demonstrate single-particle lasing around 2.3 eV with full-submicron, cesium lead bromide perovskite (CsPbBr3) crystals atop polymer-coated gold substrates at room temperature. With a large number (~100) of devices in total, we systematically study the lasing action of plasmonic test and photonic control groups. The achieved smallest plasmonic laser was 0.56 micrometer x 0.58 micrometer x 0.32 micrometer in size, ten-fold smaller than that of our smallest photonic laser. Key elements to efficient plasmonic lasing are identified as enhanced optical gain by the Purcell effect, long carrier diffusivity, a large spontaneous emission factor, and a high group index. Our results shed light on three-dimensional miniaturization of plasmonic lasers

Multiphoton 2PEF images of samples showing regions where the boundaries of the elastic lamellae layers are compromised and loose amorphous elastin has been exposed as a result of elastase digestion.

<p>Images are 275×275 µm.</p

Multiphoton images of cross sections of arteries after digestion with static stretch with 56.5, 35.6, 23.7, 15.6, 10.6, 6.8, and 5.6 µg elastin/mg wet tissue (A through G respectively) with the SHG signal (collagen, shown on left) and 2PEF signal (elastin, shown to the right) separated from a single image.

<p>There is increased fragmentation of elastic lamellae layers and collagen fibers become more disorganized with elastin removal. Images are 275×275 µm.</p

Elastin content of arterial tissue after digestion with stretch (triangles) and without stretch (circles).

<p>Lines indicate simple exponential fits. For comparisons between digestions with and without stretch, ^* P<0.05.</p

Normalized frequency vs. elastic lamellae length in arterial tissue after digestion with static stretch.

<p>Evidence of increased fragmentation is shown as distribution of lamellae lengths shifts from right (longer) to left (short fragments). All groups have a significantly decreased mean length compared to the undigested arteries, P<0.05.</p

Changes in the size of the longitudinal (A) and circumferential (B) directions of the tissue after treating with elastase with stretch.

<p>The lengths of post-digestion samples were normalized to their individual fresh tissue measurements. All digestion groups were significantly greater than the fresh condition, P<0.05.</p

Tissue tension digestion bath that allows for static stretches to be applied to both the longitudinal and circumferential directions of the sample during enzymatic degradation.

<p>Tissue tension digestion bath that allows for static stretches to be applied to both the longitudinal and circumferential directions of the sample during enzymatic degradation.</p