Coupled application of R and WetSpa models for assessment of climate change impact on streamflow of Werie Catchment, Tigray, Ethiopia

Abstract This research assesses the streamflow response of Werie River to climate change. Baseline (1980–2009) climate data of precipitation, maximum and minimum temperature were analyzed using delta based statistical downscaling approach in R software packages to predict future 90-year (2010–2099) periods under two emission scenarios of Representative Concentration Pathways (RCP) 4.5 and RCP 8.5, indicating medium and extremely high emission scenarios respectively. Generated future climate variables indicate Werie will experience a significant increase in precipitation, and maximum and minimum air temperature for both RCPs. Further, Water and Energy Transfer between Soil, Plants, and Atmosphere (WetSpa) was applied to assess the water balance of Werie River. The WetSpa model reproduced the streamflow well with performance statistics values of R2 = 0.84 and 0.85, Nash–Sutcliffe efficiency = 0.72 and 0.72, and model bias = –0.14 and –0.15 for the calibration data set of 1999–2010 and validation data of 2011–2014, respectively. Finally, by taking the downscaled future climate variables as input, WetSpa future prediction shows that there will be an increase in the Werie catchment mean annual streamflow up to 29.6% for RCP 4.5 and 35.6% for RCP 8.5 compared to the baseline period.</jats:p

QuantiFERON-TB Gold In-tube test for the diagnosis of active and latent tuberculosis in selected health facilities of Addis Ababa, Ethiopia

Abstract Objective To determine the performance of QuantiFERON-TB IN-Gold for the diagnosis active tuberculosis and latent tuberculosis. Results A total of 213 participants (136 tuberculosis suspects, 66 latently infected) were enrolled. Of 213, 21 (15.4%) of the tuberculosis suspects and 3 (4.5%) of the latent tuberculosis groups were human immunodeficiency virus infected. The sensitivity, specificity, positive and negative predictive value of QuantiFERON-TB IN-Gold for the diagnosis of active tuberculosis was 70.3% (26/37), 49.5% (49/99), 34.7% (26/75) and 83.1% (49/59) respectively. A kappa value of 0.316 (p = 0.001, 95% CI 1.605–1.609) between QuantiFERON-TB IN-Gold and tuberculin skin test were found

Assessing the Efficacy of Broad-Spectrum Antibiotics in Controlling Bacterial Contamination in the <i>In Vitro</i> Micropropagation of Ginger (<i>Zingiber officinale</i> Rosc)

Ginger (Zingiber officinale Rosc) (Zingiberaceae) is a livelihood and commercial crop in Ethiopia. But, the availability of clean and healthy planting materials has become a problem due to wilt disease, caused by Ralstonia solanacearum Biovar 3 Race 4. This problem obliged growers to seek for tens of millions of vigorous and disease-free planting materials very quickly via in vitro micropropagation of shoot tip explants. For this purpose, protocols of sterilizing shoot tip explants and controlling bacterial contamination of one Ethiopian ginger cultivar called Deribo were tested. Hence, this article reports the finding of a study that aimed at testing the (a) effectiveness of three sterilization agents, namely, 0.25% w/v RBK (composed of ridomile, bayleton, and kocide at 1 : 1 : 1 ratio), 0.50% v/v NaOCl, and 70% v/v ethanol at three different treatment times in combination with 0.25% HgCl2; (b) efficacy of four broad-spectrum antibiotics and their combinations in controlling bacterial contaminants of ginger shoot tip explants and in vitro micropropagation media; and (c) effects of the antibiotics on the shooting performances of the explants of the cultivar. A 0.50% v/v NaOCl at exposure time of 20 min followed by 0.25% HgCl2 has resulted in 80% contamination-free and 70% live explants after three weeks of incubation. Likewise, cefotaxime at 50, 100, and 200 mg/L and cefotaxime plus streptomycin at 25, 50, and 100 mg/L yielded 87 to 93% contamination-free microshoots after three weeks of culturing. The number of explants killed by the antibiotics increased with increasing the concentration of the antibiotics. Cefotaxime at 50 mg/L and cefotaxime plus streptomycin at 25 mg/L yielded significantly highest mean microshoots per explant (7.10 ± 0.36 and 7.51 ± 0.27, respectively) and mean shoot length (4.2 ± 0.26 and 3.56 ± 0.17 cm, respectively). Some of the microshoots showed some yellowing. But, they turned green and grew normal after subcultured into fresh, antibiotics-free culture media. These findings are important foundations towards developing more optimized protocols of sterilizing explants and controlling bacterial contaminants for large-scale in vitro micropropagation of the Deribo ginger cultivar.</jats:p

Assessing the Efficacy of Broad-Spectrum Antibiotics in Controlling Bacterial Contamination in the In Vitro Micropropagation of Ginger (Zingiber officinale Rosc)

Ginger (Zingiber officinale Rosc) (Zingiberaceae) is a livelihood and commercial crop in Ethiopia. But, the availability of clean and healthy planting materials has become a problem due to wilt disease, caused by Ralstonia solanacearum Biovar 3 Race 4. This problem obliged growers to seek for tens of millions of vigorous and disease-free planting materials very quickly via in vitro micropropagation of shoot tip explants. For this purpose, protocols of sterilizing shoot tip explants and controlling bacterial contamination of one Ethiopian ginger cultivar called Deribo were tested. Hence, this article reports the finding of a study that aimed at testing the (a) effectiveness of three sterilization agents, namely, 0.25% w/v RBK (composed of ridomile, bayleton, and kocide at 1 : 1 : 1 ratio), 0.50% v/v NaOCl, and 70% v/v ethanol at three different treatment times in combination with 0.25% HgCl2; (b) efficacy of four broad-spectrum antibiotics and their combinations in controlling bacterial contaminants of ginger shoot tip explants and in vitro micropropagation media; and (c) effects of the antibiotics on the shooting performances of the explants of the cultivar. A 0.50% v/v NaOCl at exposure time of 20 min followed by 0.25% HgCl2 has resulted in 80% contamination-free and 70% live explants after three weeks of incubation. Likewise, cefotaxime at 50, 100, and 200 mg/L and cefotaxime plus streptomycin at 25, 50, and 100 mg/L yielded 87 to 93% contamination-free microshoots after three weeks of culturing. The number of explants killed by the antibiotics increased with increasing the concentration of the antibiotics. Cefotaxime at 50 mg/L and cefotaxime plus streptomycin at 25 mg/L yielded significantly highest mean microshoots per explant (7.10 ± 0.36 and 7.51 ± 0.27, respectively) and mean shoot length (4.2 ± 0.26 and 3.56 ± 0.17 cm, respectively). Some of the microshoots showed some yellowing. But, they turned green and grew normal after subcultured into fresh, antibiotics-free culture media. These findings are important foundations towards developing more optimized protocols of sterilizing explants and controlling bacterial contaminants for large-scale in vitro micropropagation of the Deribo ginger cultivar

How PPAR-alpha mediated inflammation may affect the pathophysiology of chronic kidney disease

Chronic kidney disease (CKD) is a major risk factor for death in adults. Inflammation plays a role in the pathogenesis of CKD, but the mechanisms are poorly understood. Peroxisome proliferator-activated receptor alpha (PPAR-α) is a nuclear receptor and one of the three members (PPARα, PPARβ/δ, and PPARγ) of the PPARs that plays an important role in ameliorating pathological processes that accelerate acute and chronic kidney disease. Although other PPARs members are well studied, the role of PPAR-α is not well described and its role in inflammation-mediated chronic disease is not clear. Herein, we review the role of PPAR-α in chronic kidney disease with implications for the immune system

The Antioxidative Effects of Flavones in Hypertensive Disease

Hypertension is the leading remediable risk factor for cardiovascular morbidity and mortality in the United States. Excess dietary salt consumption, which is a catalyst of hypertension, initiates an inflammatory cascade via activation of antigen-presenting cells (APCs). This pro-inflammatory response is driven primarily by sodium ions (Na+) transporting into APCs by the epithelial sodium channel (ENaC) and subsequent NADPH oxidase activation, leading to high levels of oxidative stress. Oxidative stress, a well-known catalyst for hypertension-related illness development, disturbs redox homeostasis, which ultimately promotes lipid peroxidation, isolevuglandin production and an inflammatory response. Natural medicinal compounds derived from organic materials that are characterized by their anti-inflammatory, anti-oxidative, and anti-mutagenic properties have recently gained traction amongst the pharmacology community due to their therapeutic effects. Flavonoids, a natural phenolic compound, have these therapeutic benefits and can potentially serve as anti-hypertensives. Flavones are a type of flavonoid that have increased anti-inflammatory effects that may allow them to act as therapeutic agents for hypertension, including diosmetin, which is able to induce significant arterial vasodilation in several different animal models. This review will focus on the activity of flavones to illuminate potential preventative and potential therapeutic mechanisms against hypertension

Protocol for PMA-Ethiopia: A new data source for cross-sectional and longitudinal data of reproductive, maternal, and newborn health

Background: Performance Monitoring for Action Ethiopia (PMA-Ethiopia) is a survey project that builds on the PMA2020 and PMA Maternal and Newborn Health projects to generate timely and actionable data on a range of reproductive, maternal, and newborn health (RMNH) indicators using a combination of cross-sectional and longitudinal data collection.  Objectives: This manuscript 1) describes the protocol for PMA- Ethiopia, and 2) describes the measures included in PMA Ethiopia and research areas that may be of interest to RMNH stakeholders. Methods: Annual data on family planning are gathered from a nationally representative, cross-sectional survey of women age 15-49. Data on maternal and newborn health are gathered from a cohort of women who were pregnant or recently postpartum at the time of enrollment. Women are followed at 6-weeks, 6-months, and 1-year to understand health seeking behavior, utilization, and quality. Data from service delivery points (SDPs) are gathered annually to assess service quality and availability.  Households and SDPs can be linked at the enumeration area level to improve estimates of effective coverage. Discussion: Data from PMA-Ethiopia will be available at www.pmadata.org.  PMA-Ethiopia is a unique data source that includes multiple, simultaneously fielded data collection activities.  Data are available partner dynamics, experience with contraceptive use, unintended pregnancy, empowerment, and detailed information on components of services that are not available from other large-scale surveys. Additionally, we highlight the unique contribution of PMA Ethiopia data in assessing the impact of coronavirus disease 2019 (COVID-19) on RMNH.</ns3:p

Early Newborn Bath and Associated Factors among Parturient Women Who Gave Birth in the Last Month in Harar Region, Eastern Ethiopia, 2017.

Background: Neonatal thermal care is a vital intervention as newborns are susceptible to hypothermia than adults for certain reasons such as having a large body surface area, thin skin, little insulating fat, and overwhelmed thermoregulation mechanisms. Many newborn complications develop because of hypothermia due to thermal care malpractices. The leading thermal practice by women of developing countries is early bathing which predisposes newborns for life-threatening situations, such as low blood sugar levels, respiratory distress, abnormal clotting, jaundice, pulmonary hemorrhage and increased risk of developing infections. Hence, this research is aimed to provide substantial evidence regarding the women’s practices of newborn bath and the factors that determine early (&lt;24hr) bathing. Objective: The study aimed to assess the early newborn bath and its associated factors among parturient women who gave birth in the last month in the Harar region, Eastern Ethiopia, 2017. Methods: The study applied an institutional-based cross-sectional study design by recruiting 433 women. The data collectors interviewed study participants face to face at the baby immunization ward from two hospitals and four health centers. The author calculated the sample size using a double population proportion formula. A systematic sampling technique from the women’s medical registration frame was used to select the final study participants. The data collectors gathered the data using a structured questionnaire adapted from different literature, checking its consistency, reliability and validity by a pretest. Results: The response rate of this study was 99.8%. The early newborn bathing practice was found in 153 (35.4% with 95% (CI): (30.3%, 40.3%) women. Uneducated (AOR=3.12 95% CI: (2.12-5.3), no knowledge of hypothermia (AOR=4.95 95% CI: (3.10-12.2), being Primi para (AOR=3.5 95% CI: (2.5-5.6) and no utilization of newborn bed net (AOR=6.2 95% CI: (3.3-45) were statistically significant factors determining early newborn bathing practice. Conclusion: The study revealed that although the ministry implemented a good deal of awareness promotion activities, women still practice early newborn bathing. Maternal illiteracy, giving birth for the first time, knowledge deficiency related to hypothermia and newborn bed net application were among the factors which demand improvement to solve the problem. </jats:sec