The percentage of the genome recovered and the reads mapped for the each CCHFV sequences retrieved in this study.

The NGS reads were mapped to the L gene (Accession No: KC867272), Asian M gene (Accession No: MN866207), African M gene (Accession No: MH396671), and S gene (Accession No: KX013446).</p

CCHF Viral RNA, anti-CCHFV IgM and anti-CCHFV IgG kinetics of CCHFV cases.

(A) CCHF viral RNA amongst fatal and survivors (B) CCHF viral RNA amongst individual survivors at different time points (C) Anti-CCHFV-IgM antibodies amongst individual survivors at different time points and fatal cases (D) Anti-CCHFV-IgG antibodies amongst individual survivors at different time points and fatal cases.</p

The percentage of the nucleotide and amino acid divergence for each gene with respect to the reference CCHFV L, M, S segments having accession number MH396640.

(DOCX)</p

District-wise CCHF positivity in the humans, tick-pools and livestock from Gujarat State, India during the year 2019.

Image attribution: https://d-maps.com/carte.php?num_car=8623&lang=en. License attribution: https://d-maps.com/conditions.php?lang=en. The map was modified showing the district-wise CCHF human cases, tick-pools and livestock positivity in the year 2019. All the details provided in the figure are created by the author themselves using the licensed version of the online software Biorender.com.</p

Details of CCHF positive cases (n = 34), clusters of cases, clinical outcome, along with livestock and tick pool positivity from different districts of Gujarat state.

Details of CCHF positive cases (n = 34), clusters of cases, clinical outcome, along with livestock and tick pool positivity from different districts of Gujarat state.</p

Details of the time points for the follow-up of the CCHF survivors.

(DOCX)</p

Clinical presentation of fatal and survived CCHFV cases.

Clinical presentation of fatal and survived CCHFV cases.</p

Month-wise distribution of CCHF cases and deaths in Gujarat state during 2019.

Month-wise distribution of CCHF cases and deaths in Gujarat state during 2019.</p

Phylogenetic tree for the L M and S genes for the CCHFV (depicted in A, B and C respectively).

Maximum-Likelihood tree for CCHFV sequences retrieved from the clinical samples of the Gujarat state. The tree was constructed using the representative clades downloaded from Genbank using the General-time reversible model with gamma distribution as the rate parameter. The bootstrap replication of 1000 cycles was used to assess the statistical robustness of the generated tree. The scale depicts the number of base substitutions per site. Clades are marked in different colours. * indicates the fatal CCHF cases.</p

Level of Th1/Th2/Th17 cytokines in the serum samples of acute phase of infection in fatal cases (AFC) (n = 10), acute phase of infection in survived cases (ASC) (n = 13), recovered cases (RC) (n = 11) and healthy controls (HC) (n = 12) were determined using cytometric bead array (CBA) assay.

The results are expressed in scatter dot plot of individual values and data are given as Mean ± SEM (pg/ml) of (A) IL-6, (B) IL-10, (C) IFN-γ, (D) TNF-α and (E) IL-17A. Data were analyzed between groups by the non-parametric Kruskal–Wallis test followed by the post-hoc Dunn’s multiple comparison test. The horizontal lines indicate mean values. * P < 0.05; ** P < 0.01; *** P < 0.001.</p