Additional file 1 of A new concept for the production of 11C-labelled radiotracers

Additional file 1. Additional information for N-methyl-[11C]choline, L-S-methyl-[11C]methionine and [11C]acetate synthesis

Transaxial views of I-124 phantom images with PGC (right) and without PGC (left), top: with background activity, bottom: without background activity.

<p>Transaxial views of I-124 phantom images with PGC (right) and without PGC (left), top: with background activity, bottom: without background activity.</p

Simplified decay scheme of I-124 with a transition probability >3%, *maximal energy (β+: positron emission, γ: gamma emission, X: x-ray emission, ε: electron capture).

<p>Simplified decay scheme of I-124 with a transition probability >3%, *maximal energy (β+: positron emission, γ: gamma emission, X: x-ray emission, ε: electron capture).</p

RC of F-18, Ga-18 and I-124 with and without PGC as a function of sphere diameter.

<p>Reconstruction iterative with scatter correction and a Gauss-filter with a FWHM of 2 mm and a S∶BG≈10∶1.</p

Sinograms of I-124 phantom images with PGC (right) and without PGC (left), top: with background activity, bottom: without background activity.

<p>Sinograms of I-124 phantom images with PGC (right) and without PGC (left), top: with background activity, bottom: without background activity.</p

Specific activities and sphere-to-background-ratios used in PET-measurements.

<p>(SNR: Signal-to-noise ratio, RC: recovery coefficient).</p

Relative contrast of F-18, Ga-68 and I-124 with and without PGC as a function of sphere diameter.

<p>Reconstruction iterative with scatter correction and a Gauss-filter with a FWHM of 2 mm and a S∶BG≈10∶1.</p

RC of I-124 with PGC depending on post-reconstruction filtering as a function of sphere diameter.

<p>Reconstruction iterative with scatter correction and a S∶BG = 9.9∶1.</p

Uptake of ¹¹C-MET and ¹⁸F-FET by MM-cell lines in comparison to ¹⁸F-FDG.

<p>Intracellular radioactivity following incubation with ¹⁸F-FDG (A), ¹⁸F-FET (B) or ¹¹C-MET (C) was quantified using a gamma-counter. Relative uptake of background- and decay-corrected triplicate-samples was expressed as cpm per 1000 cells (mean ± sem; n=5). </p

¹¹C-MET is superior to ¹⁸F-FET and ¹⁸F-FDG in CD138⁺-plasma cells.

<p>CD138⁺-plasma cells were incubated with either ¹⁸F-FDG, ¹⁸F-FET or ¹¹C-MET for 60 min and intracellular radioactivity was quantified using a gamma-counter. Relative uptake of background- and decay-corrected samples was expressed as cpm per 1000 cells. Whenever possible, bone marrow samples were split and one half of the sample was incubated with ¹⁸F-FDG, the other with either ¹⁸F-FET (patients no 7, 10, 11) or ¹¹C-MET (patients no. 13, 16, 17, 18, 19, 21, 22, 26). (A) ¹⁸F-FDG, ¹⁸F-FET and ¹¹C-MET uptake by CD138⁺ PCs. Data from all samples analyzed are shown. (B) Direct comparison of ¹⁸F-FDG and ¹¹C-MET uptake in split samples. Lines indicate corresponding samples from one patient.</p