Quantity of the reference genes in three sets of assays as compared with the Jimai 19 calibrator (Q = E^ΔCt).

<p>Boxplot shows median, interquartile and range.</p

GeNorm analysis of four wheat endogenous reference genes assays among common wheat cultivars.

<p>Solid lines were results of analysis using all 39 cultivars, and dashed lines were results of analysis excluding the four cultivars with SNPs (Zhongguochun, Zhengmai 9023, Zhoumai 16, and Zimai 12).</p

Detected SNPs within the amplified target DNAs of <i>Acc1</i>, <i>ALMT1</i>, and <i>PKABA1</i> gene.

<p>A SNP of C>T was found at the eighth nucleotide position of <i>Acc1</i> gene forward primer in durum wheat cultivar Cannizzo; A SNP of A>C was found at the second nucleotide position of <i>ALMT1</i> gene forward primer in common wheat cultivar Zhongguochun, and one SNP of G>A was at the fourth nucleotide position of <i>ALMT1</i> gene probe in common wheat cultivar Zhengmai 9023; A SNP of G>A was found at the eleventh nucleotide position of <i>PKABA1</i> gene probe in common wheat cultivars Zhoumai 16 and Zimai 12. No SNP was found in <i>Waxy-D1</i> target region.</p