Comparison of the area under ROC curve (AUC) of three diagnostic models based upon 1: BMI; 2: BMI, GGT, TG, waist or 3: metabolic marker set.

<p>ROC statistics were based upon logistic regression analysis of 115 FLD patients and 115 controls. Compared to model 1 (AUC: 0.7609), AUC was significantly higher in both, model 2 (AUC: 0.8060, p=0.021) and model 3 (AUC: 0.8993, p=6.7×10^-6). A significant increase was also noted from model 2 to 3 (p=4.2×10^-4).</p

Metabolites showing significant associations with WG phenotype in women after correction for multiple testing.

<p>EPIC, European Prospective Investigation into Cancer and Nutrition Potsdam Study; KORA, Cooperative Health Research in the Region of Augsburg Study; PC, phosphatidylcholines; a, acyl; e, alkyl; SM, sphingomyelin.</p

Participant characteristics of weight-gaining individuals from DEGS, EPIC and KORA ^a.

<p>Participant characteristics of weight-gaining individuals from DEGS, EPIC and KORA <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157733#t001fn002" target="_blank">^a</a>.</p

Additional file 1: of pulver: an R package for parallel ultra-rapid p-value computation for linear regression interaction terms

Theory underlying pulver. This file describes the derivation of the t-value computed from the beta value divided by the standard error and the correlation value. (PDF 426 kb

Distribution of waist-hip difference in weight-gaining individuals stratified by sex for each study.

<p>DEGS, German Health Interview and Examination Survey for adults; EPIC, European Prospective Investigation into Cancer and Nutrition Potsdam Study; KORA, Cooperative Health Research in the Region of Augsburg Study.</p

Significant (p< 0.05) meta-analytic combined associations with corresponding confidence limits and p-values for both phenotypes in men.

<p>Significant (p< 0.05) meta-analytic combined associations with corresponding confidence limits and p-values for both phenotypes in men.</p

Waist-hip difference by study and their correlation with weight change.

<p>Waist-hip difference by study and their correlation with weight change.</p

Ratio of total lysophosphatidylcholines to total phosphatidylcholines.

<p>The displayed concentration values represent the mean of the samples of all 19 participants at the respective pre-analytical condition. The error bars represent one standard deviation based on the 19 measurements. The rof total lysophosphatidylcholines to total phosphatidylcholines (tLyso/tPC) in samples treated by different pre-storage handling conditions increases with time during RT treatment.</p

Metabolites with significant concentration changes due to the pre-storage handling conditions of the samples.

<p>¹: metabolites displayed significant p-values (in bold) of the linear regression model either for temperature alone (temp), for temperature and interaction (time:temp) or for time (time), temperature and interaction; the column on the right indicates the direction of the observed concentration changes.</p><p>Metabolites with significant concentration changes due to the pre-storage handling conditions of the samples.</p

Metabolite ratio importance plot.

<p>The importance for each metabolite ratio in classifying samples into ‘good’ or ‘bad’ pre-storage handling conditions was assessed by the average decrease in classification accuracy found in 500 Random Forest analysis iterations (the full names of the metabolites can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121495#pone.0121495.s003" target="_blank">S1 Table</a>).</p