46 research outputs found

    Supplementary Online Material

    No full text
    More information on the structure characterization and magnetization data of the Fe5GeTe2 flak

    Additional file 1 of Development of a novel immune-related lncRNA prognostic signature for patients with hepatocellular carcinoma

    No full text
    Additional file 1: Table 1. Clinical characteristics of hepatocellular carcinoma in train and validation cohort

    Additional file 3 of Development of a novel immune-related lncRNA prognostic signature for patients with hepatocellular carcinoma

    No full text
    Additional file 3: Supplementary Figure 1. (A) The best-fit OS-related lncRNAs were chosen by Lasso regression analysis. (B) The Lasso regression was performed with the optimal value of λ. (C-D) Distribution of risk scores, survival status. Supplementary Figure 2. (A-D) PCA among all genes, immune genes, immune LncRNA, and risk immune LncRNA

    Suppression of Graft Regeneration, Not Ischemia/Reperfusion Injury, Is the Primary Cause of Small-for-Size Syndrome after Partial Liver Transplantation in Mice

    No full text
    <div><p>Background</p><p>Ischemia/reperfusion injury (IRI) is commonly considered to play a crucial role in the pathogenesis of small-for-size syndrome (SFSS) after liver transplantation. Rapid regeneration is also considered essential for the survival of SFS grafts.</p><p>Methods</p><p>Mouse models of full-size orthotopic liver transplantation, 50% partial liver transplantation and 30% partial liver transplantation were established. Survival rate and serum alanine aminotransferase were observed. IRI was assessed by hepatic pathologic alterations, apoptosis and necrosis. Regeneration response was detected by mitotic index, BrdU incorporation and PCNA, Cyclin D1 and Cyclin E expression. The expression of mTOR, AKT, ERK, JNK2 and p70S6K, also involved in regeneration signaling pathways, were analyzed as well.</p><p>Results</p><p>30% partial liver graft resulted in a significantly low 7-day survival rate (P = 0.002) with no marked difference in tissue injury compared with the 50% partial graft group. Serum alanine aminotransferase levels were not significantly different between partial transplantation and full-size transplantation. Western blot analysis of caspase-3 and TUNEL staining also indicated no significant difference in apoptosis response between 30% partial transplantation and half-size or full-size transplantation (P = 0.436, P = 0.113, respectively). However, liver regeneration response indicators, mitotic index (P<0.0001) and BrdU (P = 0.0022), were markedly lower in 30% LTx compared with 50% LTx. Suppressed expression of PCNA, cyclin D1, cyclin E, mTOR, JNK2, AKT, ERK and p70S6K was also detected by western blot.</p><p>Conclusions</p><p>Liver regeneration is markedly suppressed in SFSS, and is more likely the primary cause of SFSS, rather than ischemia/reperfusion injury. Therapy for recovering graft regeneration could be a potentially important strategy to reduce the incidence of SFSS.</p></div

    Decreased survival rate after transplantation of small-for-size liver grafts.

    No full text
    <p>Mice were observed 7 days postoperatively for survival. Group sizeswere 8–10 each in the FSG, HSG and TSG groups, respectively. P<0.05 by the Kaplan-Meier test. At 48h post-transplantation, when the samples were taken, the 1-day survival rate of TSG was 100%.</p

    Data_Sheet_1_Computational screening for new neuroprotective ingredients against Alzheimer's disease from bilberry by cheminformatics approaches.docx

    No full text
    Bioactive ingredients from natural products have always been an important resource for the discovery of drugs for Alzheimer's disease (AD). Senile plaques, which are formed with amyloid-beta (Aβ) peptides and excess metal ions, are found in AD brains and have been suggested to play an important role in AD pathogenesis. Here, we attempted to design an effective and smart screening method based on cheminformatics approaches to find new ingredients against AD from Vaccinium myrtillus (bilberry) and verified the bioactivity of expected ingredients through experiments. This method integrated advanced artificial intelligence models and target prediction methods to realize the stepwise analysis and filtering of all ingredients. Finally, we obtained the expected new compound malvidin-3-O-galactoside (Ma-3-gal-Cl). The in vitro experiments showed that Ma-3-gal-Cl could reduce the OH· generation and intracellular ROS from the Aβ/Cu2+/AA mixture and maintain the mitochondrial membrane potential of SH-SY5Y cells. Molecular docking and Western blot results indicated that Ma-3-gal-Cl could reduce the amount of activated caspase-3 via binding with unactivated caspase-3 and reduce the expression of phosphorylated p38 via binding with mitogen-activated protein kinase kinases-6 (MKK6). Moreover, Ma-3-gal-Cl could inhibit the Aβ aggregation via binding with Aβ monomer and fibers. Thus, Ma-3-gal-Cl showed significant effects on protecting SH-SY5Y cells from Aβ/Cu2+/AA induced damage via antioxidation effect and inhibition effect to the Aβ aggregation.</p

    Western Blot for expression of caspase-3.

    No full text
    <p>Alterations of caspase-3 expression after liver transplantation. Livers were collected 48 h post-transplantation. Caspase-3 was detected by immunoblotting. Representative gels are shown.</p

    Western Blot results of the four groups.

    No full text
    <p><b>A:</b> Livers were recovered at 48-transplantation. PCNA, cyclin D1 (CyD1) and cyclin E (Cy E) were detected by immunoblotting. Representative gels of 4 livers/group are shown. <b>B:</b>Alterations of EGFR downstream signaling molecules after liver transplantation. Livers were collected 48 h post-transplantation. Phospho-Akt, phospho-mTOR, phospho-p70S6 kinase (p-p70S6K), phospho-ERK, phospho-JNK and actin were detected by immunoblotting. Representative gels are shown.</p
    corecore