A Coumarin-Based Array for the Discrimination of Amyloids

Self-assembly of misfolded proteins can lead to the formation of amyloids, which are implicated in the onset of many pathologies including Alzheimer’s disease and Parkinson’s disease. The facile detection and discrimination of different amyloids are crucial for early diagnosis of amyloid-related pathologies. Here, we report the development of a fluorescent coumarin-based two-sensor array that is able to correctly discriminate between four different amyloids implicated in amyloid-related pathologies with 100% classification. The array was also applied to mouse models of Alzheimer’s disease and was able to discriminate between samples from mice corresponding to early (6 months) and advanced (12 months) stages of Alzheimer’s disease. Finally, the flexibility of the array was assessed by expanding the analytes to include functional amyloids. The same two-sensor array was able to correctly discriminate between eight different disease-associated and functional amyloids with 100% classification

Additional file 1 of Tranexamic acid alters the immunophenotype of phagocytes after lower limb surgery

Additional file 1: Supplementary Figure 1. Gating strategy used to identify myeloid cells. Supplementary Figure 2. Gating strategy used to identify B cells. Supplementary Figure 3. Gating strategy used to identify NK cells. Supplementary Figure 4. Comparison of results for TXA-treated patients in the randomised and after the randomised recruitment. Supplementary Figure 5. Plasmin-antiplasmin (PAP) complex as a readout for plasmin generation