Debecker & Stoks_POLS data_Ecological Monographs_2018

Every line is one subject (Ischnura elegans larva). Every column is a variable, either measured or manipulated. The columns "Latitude", "Temperature" and "Zinc" are the treatments used in the experiment. The endpoints used in the paper are indicated in yellow. Other columns are added for completeness. Please consult the paper for more information

Carry-Over Effects Across Metamorphosis of a Pesticide on Female Lifetime Fitness Strongly Depend on Egg Hatching Phenology: A Longitudinal Study under Seminatural Conditions

Current ecological risk assessment of pesticides fails to protect aquatic biodiversity. For the first time, we tested two potential reasons for this failure with regard to carry-over effects across metamorphosis: their dependence on hatching period, and the lack of studies quantifying adult fitness under seminatural conditions. Using the damselfly <i>Coenagrion puella</i> sampled from six populations, we designed an outdoor longitudinal one-year study starting from the egg stage. We exposed the aquatic larvae to the pesticide esfenvalerate (0.11 μg/L) during the initial microcosm part. Next, we monitored the lifetime fitness of the terrestrial adults in an insectary. Exposure to the pesticide negatively impacted not only larval traits, but also drastically reduced lifetime mating success of adult females. The impact of this postmetamorphic effect of the pesticide on the population level was three times more important than the effects in the larval stage. Importantly, this carry-over effect was only present in females that hatched early in the season, and was not mediated by metamorphic traits (age and mass at emergence). We provide proof-of-principle under seminatural conditions for two potential pitfalls that need to be considered when improving risk assessment: carry-over effects on adult fitness can (i) be much more important than effects during the larval stage and may not be captured by metamorphic traits, and (ii) be strongly modulated by egg hatching dates

Effects of chlorpyrifos, food level and temperature on development time and mass at emergence.

<p>Mean larval development time (A, C) and mass at emergence (B, D) of <i>E. cyathigerum</i> damselflies as a function of exposure to the pesticide chlorpyrifos and food level (A, B) and temperature (C, D). Given are least-squares means ± 1 SE. Open symbols represent the pesticide-free control animals, closed symbols represent the pesticide-exposed larvae.</p

data Janssens & Stoks F.E.

original dataset with different response variables (life history and physiology) covered in the article published in Functional Ecology

Effects of chlorpyrifos, food level and temperature on chill cold resistance.

<p>Mean chill coma recovery times of <i>E. cyathigerum</i> damselflyes as a function of exposure to the pesticide chlorpyrifos and (A) food level and (B) temperature. Given are least-squares means ± 1 SE. Open symbols represent the pesticide-free control animals, closed symbols represent the pesticide-exposed larvae.</p

Effect of predation risk on swimming speed, abdominal muscle mass and oxidative damage.

<p>Mean (+1 SE) swimming speed (A), abdominal muscle mass (B), oxidative damage to proteins (carbonyl levels) (C) and oxidative damage to lipids (MDA levels) (D) of <i>C. puella</i> damselfly larvae as a function of experimental run and exposure to predation risk.</p

Covariation patterns between swimming speed and abdominal muscle mass and oxidative damage.

<p>Covariation patterns between swimming speed and (A) abdominal muscle mass, (B) oxidative damage to proteins and (C) oxidative damage to lipids in <i>C. puella</i> damselfly larvae.</p

Effects of chlorpyrifos, food level and temperature on survival.

<p>Percentage of surviving <i>E. cyathigerum</i> damselflies as a function of exposure to the pesticide chlorpyrifos and food level (A) and temperature (B).</p

Appendix B. A figure showing selected behavioral variables of the seven Lestes species in the behavior experiment under differential predation risk.

A figure showing selected behavioral variables of the seven Lestes species in the behavior experiment under differential predation risk

Appendix C. A figure showing digestive variables of the four Lestes studied in the growth in isolation experiment.

A figure showing digestive variables of the four Lestes studied in the growth in isolation experiment