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    Evaluation of antigen based rapid diagnostic test in comparison to RT PCR in diagnosis of Sars CoV2 with respect to duration of illness and Ct value of corresponding RT PCR

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    Background: SARS-CoV-2 is the third highly pathogenic corona virus introduced into mankind after Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) and Middle East Respiratory Syndrome coronavirus (MERS-CoV) in twenty-first century for which the development and validation of rapid and easy-to-perform diagnostic methods are of high priority. Objective: In this study we evaluated performance characteristics of RAT, the STANDARD Q COVID19 Ag by SD-Biosensor for rapid detection of SARS CoV 2. Material and methods: Samples were collected from 1168 patients and we performed both RAT and RT PCR and the results of RAT were compared with that of RT PCR as gold standard. Result: Detection rates of SARS CoV-2 by RAT and RT-PCR were 19.17% and 29.53%, respectively; false positivity rate was 2.67%.False positive and false negative rate was 2.6% and 13.45% respectively.RAT sensitivity, specificity, negative and positive predictive values were 63.18%, 99.27%, 97.32% and 86.54% respectively. Statistical analysis considered the calculation of sensitivity, specificity, positive predictive value, negative predictive value using standard formulae. Conclusion: A high sensitivity, specificity, positive predictive value and fairly high negative predictive value of RAT might prove to be promising in situations where pre-test probability of having infection is high