Trehalose activated the p38 MAPK signaling pathway in cryopreserved valve cells.

(A, B and C) Western blotting using p-mTOR (Ser2448), mTOR, p38 MAPK and p-p38 MAPK antibodies on cryopreserved valve cells treated with trehalose (0.1 mol/L) or DMSO for 16 weeks. Data are expressed as the mean ± SD (n = 20 per group). ***P < 0.001 versus the DMSO group.</p

Trehalose improved cell glucose consumption and decreased MDA content of cryopreserved valve cells.

<p>Valve cells were treated with DMSO or trehalose (0–0.20 mol/L). (A) Glucose consumption, valves were cryopreserved for 4 weeks. (B) MDA, valves were cryopreserved for 4 weeks. (C) Glucose consumption, valves were cryopreserved for 16 weeks. (D) MDA, valves were cryopreserved for 16 weeks. Data are expressed as the mean ± SD (<i>n</i> = 4 per group). *<i>P</i> < 0.05, **<i>P</i> < 0.01 versus the DMSO group.</p

Expression levels of miR-32 and miR-221 in cryopreserved valve cells.

<p>Twenty pairs of DMSO samples and trehalose samples were analyzed by qRT-PCR and normalized to U6 expression. (A and C) The bars represented relative miR-32 and miR-221 expression with the ratio of levels in DMSO samples to those in trehalose samples on a logarithmic scale. (B and D): miR-32 and miR-221 expression levels in DMSO samples and trehalose samples were compared with a paired Student’s t-test. *<i>P</i> < 0.05 versus DMSO group. (E, F and G): correlation analysis between miR-32 expression and cleaved caspase-3 level, LC3A/B II/I ratio and p38 MAPK phosphorylation. (H and J): correlation analysis between miR-221 expression and cleaved caspase-3 level, LC3A/B-II/I ratio and p38 MAPK phosphorylation. Spearman's analysis was used in the correlation analysis.</p

Effect of trehalose on autophagy in cryopreserved valve cells.

<p>(B, C, E and F) Western blotting using LC3A/B-II/I, p62, ATG-5 and ATG-7 antibodies on cryopreserved valve cells treated with trehalose (0.1 mol/L) or DMSO for 16 weeks. Data are expressed as the mean ± SD (<i>n</i> = 20 per group). **<i>P</i> < 0.01, ***<i>P</i> < 0.001 versus the DMSO group.</p

Effect of trehalose on apoptosis and ERS in cryopreserved valve cells.

<p>(A and B) Western blotting using a cleaved caspase-3 antibody on cryopreserved valve cells treated with trehalose (0.1 mol/L) or DMSO for 16 weeks. (C, D and E) Western blotting using GRP78 and CHOP antibodies on cryopreserved valves treated with trehalose (0.1 mol/L) for 16 weeks. Data are expressed as the mean ± SD (<i>n</i> = 20 per group). **<i>P</i> < 0.01, ***<i>P</i> < 0.001 versus the DMSO group.</p

Additional file 1: Figure S1. of Raspberry-like PS/CdTe/Silica Microspheres for Fluorescent Superhydrophobic Materials

TEM images of PS/CdTe/silica microspheres with PS cores (651 nm)

How do visitors’ perceptions differ from the supply of cultural ecosystem services in urban parks? A case study from Beijing

As urban green spaces, parks can provide rich cultural ecosystem services (CES), enhancing the well-being of those living in urban areas. Understanding how people perceive the CES supplied by parks and identifying differences with their supply is crucial for decision-makers and urban planners. In this study, we conducted a quantitative assessment of CES by combining an expert field investigation of parks with visitor questionnaires in the Three Hills and Five Gardens area of Beijing, China. Our assessment system comprised five categories of CES (landscape aesthetics, historical heritages, education, recreation, health and fitness) and eleven indicators. We identified differences between CES supply and perceptions and noted that such discrepancies additionally vary by CES type. We found that multiple CES are interdependent and interwoven. In addition, we discovered that perceptions of historical heritages service are particularly dependent on supply, while perceptions of health and fitness service are relatively independent of the supply. We explored the reasons behind these differences, finding that the visibility and prestige of historical heritages as well as the positioning and the overall condition of parks can affect visitors’ perceptions. Our assessment can be used to guide the optimization of parks so that they may provide higher-quality CES for the public.</p

Data_Sheet_1_Longitudinal Associations Between Household Solid Fuel Use and Handgrip Strength in Middle-Aged and Older Chinese Individuals: The China Health and Retirement Longitudinal Study.pdf

BackgroundHousehold solid fuel have been associated with changes of handgrip strength (HGS). However, no study has explored the longitudinal associations between household solid fuel use and HGS. Thus, the aim of our cohort study was to investigate the longitudinal associations between household fuel use and HGS.MethodsThe study was based on the China Health and Retirement Longitudinal Study. A handheld dynamometer was used to measure HGS. Household fuel use statuses were collected using questionnaires. Analyses of covariance were performed to examine the associations between household fuel use and HGS.ResultsThe study included 9,382 participants during a 4-year follow-up. The participants who used solid fuel for cooking had more decreases of HGS than those who used clean fuel (P ConclusionUsing household solid fuel for cooking but not heating was associated with more decreases in HGS. Proper ventilation and clean fuel should be promoted for public health.</p

Western blots for neural stem cell and neuroprogenitor cell proteins in piglet forebrain.

<p><b>A</b>. Nestin immunoreactivity in forebrain subventricular zone (SVZ) and olfactory bulb (OB) SVZ. Lower four blots show immunoreactivities for Dlx2 (<b>B</b>), musashi (<b>C</b>), doublecortin (<b>D</b>), and PSA-NCAM (<b>E</b>) in OB SVZ subcellular extracts (membrane, soluble and crude nuclear) at postnatal day (PND) 1 and PND 30. Molecular weight standards (in kDa) are shown at right side of the blots. <b>F</b>. Representative Ponceau S-stained nitrocellulose membrane is shown for protein loading. <b>G</b>. Graph showing the densitometry quantification of the levels of immunoreactivity for Dlx-2, musashi, doublecortin, and PSa-NCAM in membrane (Dlx-2 and PSA-NCAM) and soluble (musashi and doublecortin) fractions in the OB periventricular region at PND1 and PND30. Values are mean ± SD. Single asterisk denotes musashi PND30 vs PND1 p < 0.05. Double asterisk denotes PSA-NCAM PND1 vs PND30 p < 0.001.</p

sj-pdf-1-het-10.1177_09603271211065972 – Supplemental Material for Propofol suppresses proliferation, migration, invasion, and tumor growth of liver cancer cells via suppressing cancer susceptibility candidate 9/phosphatase and tensin homolog/AKT serine/threonine kinase/mechanistic target of rapamycin kinase axis

Supplemental Material, sj-pdf-1-het-10.1177_09603271211065972 for Propofol suppresses proliferation, migration, invasion, and tumor growth of liver cancer cells via suppressing cancer susceptibility candidate 9/phosphatase and tensin homolog/AKT serine/threonine kinase/mechanistic target of rapamycin kinase axis by Qing Chang, Jun Wu, Yang An, Haiyan Liu and Yang Sun in Human & Experimental Toxicology</p