48 research outputs found

    Effects of <i>csnk-1</i> transgenes on the Sisi phenotype of <i>csnk-1(lf)</i> mutants in 5 mM NaI.

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    Effects of csnk-1 transgenes on the Sisi phenotype of csnk-1(lf) mutants in 5 mM NaI.</p

    Deleterious genetic variations detected by whole-genome sequencing in the mapped region of the <i>mac397</i> isolate.

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    Ratios of mutated sequences are shown in parentheses. A ratio of 1.00 suggests homozygous, and a ratio less than 1.00 suggests heterozygous. A: adults. ND: not determined. (TIFF)</p

    Phenotypic analyses of <i>csnk-1(lf)</i> mutants.

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    (A) Number of all eggs laid per adult. Results were based three biological replicates, with three animals per replicate. Colors represent different replicates. Statistics: two-tailed unpaired Student’s t-test. *: p csnk-1(lf) homozygous mutants. All eggs laid by a single adult on regular NGM agar plates were examined. Results were based on three biological replicates, with three animals per replicate. Statistics: two-tailed unpaired Student’s t-test. ****: p csnk-1(lf) mutants. Arrows indicate attached cuticles. (E) Quantification of young adults (24 hrs after mid-L4 larval stage) with molting defects. Results were based on three biological replicates, with 100 animals analyzed in each replicate. Statistics: two-tailed unpaired Student’s t-test. **: p p csnk-1(lf)/+ and csnk-1(lf) mutants labeled by a DPY-7::sfGFP reporter. (H) Percentage of young adults positively stained by the nuclear dye Hoechst 33258. Results were based on three biological replicates, with 59–141 animals in each replicate. Statistics: two-tailed unpaired Student’s t-test. ****: p csnk-1(lf) L4 animals stained with DCFDA. Pictures were taken with the same exposure time of 600 ms and fluorescent intensity of each animal was measured using ImageJ. (K) Quantification of DCFDA fluorescent signals of individual L4 animals. Results were based on three biological replicates, with 21–100 animals in each replicate. Statistics: two-tailed unpaired Student’s t-test. ****: p (TIFF)</p

    Nonallelic noncomplementation interaction between <i>csnk-1</i> and <i>tsp-15</i>, <i>bli-3</i> or <i>doxa-1</i>.

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    Nonallelic noncomplementation interaction between csnk-1 and tsp-15, bli-3 or doxa-1.</p

    CSNK-1::mCherry does not colocalize with GFP and DOXA-1::GFP does not colocalize with mCherry.

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    (A, B, C) A transgenic L3 larvae co-expressing GFP and CSNK-1::mCherry in epithelial cells. (D, E, F) A transgenic 3-fold embryo co-expressing DOXA-1::GFP and mCherry in epithelial cells. For unclear reason, DOXA-1::GFP was strongly expressed in embryos but was not visible at larval stages in these transgenic lines. We therefore observed whether DOXA-1::GFP colocalizes with mCherry in embryos. (TIFF)</p

    PCR primers for generating the listed DNA fragments.

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    PCR primers for generating the listed DNA fragments.</p

    Characterization of <i>csnk-1</i>.

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    (A) csnk-1 gene structure (based on wormbase.org). The positions of mac397, mac494 and mac495 mutations are indicated. The position of the sgRNA used for CRISPR/Cas9-based mutagenesis is shown as a red bar. (B) Percentage of L1 larva that grew into adults on plates with 5 mM NaI. Results were based on two biological replicates. 100 L1 larva were analyzed in each replicate. Statistics: two-tailed unpaired Student’s t-test. *: p csnk-1p::GFP transgene in adults. (C) Fluorescent picture of a transgenic adult. The head, tail and vulva are indicated. (D-H) Higher-resolution pictures of transgenic adults. Cells with obvious GFP expression are indicated.</p
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