MOESM6 of A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans

Additional file 6: Fig. S6. Screen of FjoAcXEA activity towards selected pNP alkyl esters showing activity on short chain (< C4) substrates consistent with esterase rather than lipase activity. Reactions (200 µL) contained 0.5 µg of FjoAcXE, 50 mM HEPES (pH 8.0), and 2 mM of each substrate. Absorbance at 410 nm was measured after 2 h at 30 °C. pNP acetate (C2), pNP butyrate (C4), pNP hexanoate (C6), pNP octanoate (C8), pNP decanoate (C10), pNP dodecanoate (C12), pNP myristate (C14), and pNP palmitate (C16). n = 3; error bars correspond to standard deviation

MOESM3 of A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans

Additional file 3: Fig. S3. Purified FjoAcXE is approximately 45.2 kDA

MOESM4 of A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans

Additional file 4: Fig. S4. FjoAcXE activity screen against 0.5% (w/v) of selected polysaccharides. Reactions (50 µL) contained 5 µg of FjoAcXE, 50 mM HEPES (pH 8.0), and 0.5% w/v of each substrate, and were incubated for 16 h at 30 °C. Reducing sugars were measured using 1% final PAHBAH reagent [58]. BEX = beechwood xylan (Sigma, X4252); OSX = oat spelt xylan (Sigma, X0627); CMC = carboxymethylcellulose (Megazyme, P-CMC4 M); β-glucan (low viscosity; from barley; Megazyme, P-BGBL); starch (from corn; Sigma-Aldrich, S4126); pectin (from apple; Sigma, 76282); WAX = wheat arabinoxylan (high viscosity; Megazyme, P-WAXYH); arabinan (from sugarbeet; Megazyme, P-ARAB); glucomannan (low viscosity; from konjac; Megazyme, P-GLCML); galactomannan (from guar, GD28; Megazyme, enzyme modified); xyloglucan (amyloid, from tamarind seed; Megazyme, P-XYGLN); arabinogalactan (acacia gum, Sigma, G9752)

MOESM5 of A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans

Additional file 5: Fig. S5. FjoAcXE activity screen against selected pNP substrates. Reactions (200 µL) contained 5 µg of FjoAcXE, 50 mM HEPES (pH 8.0), and 2 mM of each substrate. Absorbance was measured after 2 h at 30 °C