MOESM2 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 2. Logistic fitting results

MOESM1 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 1. Additional methods and results

MOESM4 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 4. Differentially expressed genes for M2 vs SPT15

MOESM3 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 3. RPKM values and fold changes of genes in the central carbon metabolic network

MOESM5 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 5. GO enrichment of differentially expressed genes for M2 vs SPT15

MOESM6 of Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus

Additional file 6. KEGG enrichment of differentially expressed genes for M2 vs SPT15

L'Auto-vélo : automobilisme, cyclisme, athlétisme, yachting, aérostation, escrime, hippisme / dir. Henri Desgranges

13 janvier 19371937/01/13 (A38,N13176)

Universal Parameter Optimization of Density Gradient Ultracentrifugation Using CdSe Nanoparticles as Tracing Agents

Density gradient ultracentrifugation (DGUC) has recently emerged as an effective nanoseparation method to sort polydispersed colloidal NPs mainly according to their size differences to reach monodispersed fractions (NPs), but its separation modeling is still lack and the separation parameters’ optimization mainly based on experience of operators. In this paper, we gave mathematical descriptions on the DGUC separation, which suggested the best separation parameters for a given system. The separation parameters, including media density, centrifuge speed and time, which affected the separation efficiency, were discussed in details. Further mathematical optimization model was established to calculate and yield the “best” (optimized) linear gradient for a colloidal system with given size and density. The practical experiment results matched well with theoretical prediction, demonstrating the DGUC method, an efficient, practical, and predictable separation technique with universal utilization for colloid sorting

Layered double hydroxide-based electrocatalysts for the oxygen evolution reaction: identification and tailoring of active sites, and superaerophobic nanoarray electrode assembly

The electrocatalytic oxygen evolution reaction (OER) is a critical half-cell reaction for hydrogen production via water electrolysis. However, the practical OER suffers from sluggish kinetics and thus requires efficient electrocatalysts. Transition metal-based layered double hydroxides (LDHs) represent one of the most active classes of OER catalysts. An in-depth understanding of the activity of LDH based electrocatalysts can promote further rational design and active site regulation of high-performance electrocatalysts. In this review, the fundamental understanding of the structural characteristics of LDHs is demonstrated first, then comparisons and in-depth discussions of recent advances in LDHs as highly active OER catalysts in alkaline media are offered, which include both experimental and computational methods. On top of the active site identification and structural characterization of LDHs on an atomic scale, strategies to promote the OER activity are summarised, including doping, intercalation and defect-making. Furthermore, the concept of superaerophobicity, which has a profound impact on the performance of gas evolution electrodes, is explored to enhance LDHs and their derivatives for a large scale OER. In addition, certain operating standards for OER measurements are proposed to avoid inconsistency in evaluating the OER activity of LDHs. Finally, several key challenges in using LDHs as anode materials for large scale water splitting, such as the issue of stability and the adoption of membrane–electrode-assembly based electrolysers, are emphasized to shed light on future research directions.</p

Electronic coupling strategy to boost water oxidation efficiency based on the modelling of Trimetallic Hydroxides Ni1-x-yFexCry(OH)2: from theory to experiment

Developing low-cost yet highly efficient earth-abundant electrocatalysts for oxygen evolution reaction (OER) is of great significance for industrial scale water splitting for clean hydrogen production, as well as for rechargeable metal-air batteries. In searching for advanced catalysts, it is equally important to fundamentally understand working mechanism and be able to rationally design and manipulate catalytic sites. Starting from density functional theory (DFT) calculations as a guidance, our theoretical model revealed that chromium substitution in nickel-iron hydroxides (Ni1-xFex(OH)2) not only accelerated the charge transfer but also regulated the adsorption energy of OER intermediates such to achieve optimal binding strength. Experimentally, chromium was doped into the laminate of Ni1- xFex(OH)2, resulting in enhanced catalytic performance for oxygen evolution reaction, which confirmed the predictions from the theoretical data. The porous and ultra-thin ternary Ni1-xyFexCry(OH)2 electrocatalysts were grown directly on a nickel foam (NF) substrate, with an optimum composition Ni0.66Fe0.27Cr0.07(OH)2/NF identified, which exhibited a superior OER performance, i.e., achieving a significant current density of 10 mA cm-2 at a low overpotential of 231 mV, a small Tafel slope (31 mV dec-1) and an excellent stability at a highly oxidative potential of 1.68 V vs RHE in alkaline electrolyte. The comprehensive study involving both theoretical and experimental results in this work provides an insightful guidance in designing efficient OER catalysts for chemical and electrical energy conversion and storage