Phylogenetic tree of candidate <i>BminCSP</i> with other Dipteran CSP sequences.

Dmel: Drosophila melanogaster; Bdor: Bactrocera dorsalis; Cqui: Culex quinquefasciatus; Csty: Calliphora stygia; Gmor: Glossina morsitans morsitans; Dant: Delia antiqua; Scal: Stomoxys calcitrans Asin: Anopheles sinensis; Agam: Anopheles gambiae; Lsat: Liriomyza sativae.</p

Phylogenetic tree of candidate <i>BminORs</i> with known Dipteran OR sequences and the candidate <i>BminOR</i> genes expression pattern in EF, MF and MM.

Dmel: D melanogaster; Bdor: B dorsalis; Agam: A gambiae.</p

Phylogenetic tree of candidate <i>BminIRs</i> with known Dipteran IR sequences and the candidate <i>BminIR</i> genes expression pattern in EF, MF and MM.

Dmel: D melanogaster; Bdor: B dorsalis.</p

Presentation_1_The Application of a Meiocyte-Specific CRISPR/Cas9 (MSC) System and a Suicide-MSC System in Generating Inheritable and Stable Mutations in Arabidopsis.pdf

<p>The CRISPR/Cas9 system has been widely used for generating targeted mutations in various species. In Arabidopsis, it largely relies on the edited cells where the Cas9 protein performs its activity to obtain heritable and stable mutated lines. Here, we designed an improved CRISPR/Cas9 system, named as the MSC (meiocyte-specific CRISPR/Cas9) system, in which the Cas9 expression is driven by an experimentally approved meiocyte-specific promoter (AtDMC1 promoter). Two endogenous genes, including vegetative gene AtDET2 and reproductive gene AtDMC1, were targeted. We obtained heterozygous T1 plants for targeted genes with high efficiency (64%). In the T2 generation, the homozygous plants were abundant with high efficiency (37%). Analysis of Sanger sequencing results of T2 generation revealed that heritable gene mutations were high (52%). Moreover, we showed that the MSC system could sufficiently delete a middle size DNA fragment (∼500 bp) between two cleavage sites with a high rate (64.15%) in the T1 plants, providing direct evidence for making complete knock-out or certain domain-depletion mutations. In addition, we further made a suicide-MSC system, which can edit the targeted endogenous gene and the exogenous Cas9 gene simultaneously, not only successfully avoiding the further destroy of alleles brought in by molecular complementary or genic allelic test, but also maintaining the stable mutated alleles for functional studies. In short, the two systems provide new approaches to generate mutations for gene functional studies.</p

Time expression patterns of candidate <i>B</i>. <i>minax</i> olfaction genes.

Error bars represent 1 SE.</p

Tissue- and sex-specific expression patterns of candidate <i>B</i>. <i>minax</i> olfaction genes.

The X-axis represents the different tissues of B. minax. FA: female antennae; MA: male antennae; H: head (without antennae); T: thorax; A: abdomen; L: legs; W: wings. Error bars represent the standard error of the measurement.</p

Phylogenetic tree of candidate <i>BminOBPs</i> with known Dipteran OBP sequences and the candidate <i>BminOBP</i> genes expression pattern in newly emerged females (EF), sexually mature females (MF), and sexually mature males (MM).

Dmel: D melanogaster; Bdor: B dorsalis; Agam: A gambiae.</p

EAG response of <i>B</i>. <i>minax</i> antennae to D-limonene for the sexually mature females.

A: qRT-PCR result of BminCSP gene silencing; B: qRT-PCR result of BminOBP21 gene silencing; C, D: Relative EAG response of GFP- and RNAi-treated female to D-limonene.</p

Synthesis of CF₃CH₂‑Containing Indolines by Transition-Metal-Free Aryltrifluoro­methylation of Unactivated Alkenes

With an unactivated double bond as the radical acceptor, allyl amines underwent a metal-free trifluoromethylation/cyclization cascade with CF3SO2Na (Langlois’ reagent), affording CF3CH2-containing indolines and tetrahydro­isoquinolines, whose practical syntheses are significant challenges. This protocol features mild conditions, low cost, and a broad substrate scope

Synthesis of CF₃CH₂‑Containing Indolines by Transition-Metal-Free Aryltrifluoro­methylation of Unactivated Alkenes

With an unactivated double bond as the radical acceptor, allyl amines underwent a metal-free trifluoromethylation/cyclization cascade with CF3SO2Na (Langlois’ reagent), affording CF3CH2-containing indolines and tetrahydro­isoquinolines, whose practical syntheses are significant challenges. This protocol features mild conditions, low cost, and a broad substrate scope