Image_1_Combined effects of toxic Karenia mikimotoi and hypoxia on the juvenile abalone Haliotis discus hannai.tif

Eutrophication in aquaculture areas concurrently leads to a high incidence of dissolved oxygen deficiency and toxic algal blooms. The combined effects of hypoxia and typical toxic algae on cultured organisms should be given sufficient consideration. Abalone breeding in China has greatly suffered from hypoxia and toxic Karenia mikimotoi blooms for many years. In this study, the individual and combined effects of the toxic dinoflagellate, K. mikimotoi, and hypoxia on juvenile abalone were determined based on abalone survival and oxidative stress indicators in their gills, hepatopancreas and hemolymph. The results showed that at a density of 106 to 3×107 cells/L, K. mikimotoi alone had a negligible influence on the survival of juvenile abalone under sufficient dissolved oxygen (DO) conditions. The 24 h-half lethal concentration (LC50) of DO alone for juvenile abalone was 0.75 mg/L in seawater. When K. mikimotoi was added at a density of 3×106 cells/L, the 24 h-LC50 of DO for juvenile abalone significantly increased to 2.59 mg/L, indicating obvious synergistic effects. The individual effects of hypoxia or K. mikimotoi on the oxidative stress indicators were limited, and only the superoxide dismutase (SOD) activity in the abalone gills significantly decreased under K. mikimotoi stress. However, the combined stress of hypoxia and K. mikimotoi led to significant changes in the antioxidant indicators in all tested tissues. The SOD activity in gills and hepatopancreas decreased, while the SOD and catalase (CAT) activity and malondialdehyde (MDA) content in the hemolymph increased due to the combined stress of hypoxia and K. mikimotoi. These results illustrated that the synergistic effects of hypoxia and K. mikimotoi caused serious oxidative damage in abalone and that the hemolymph exhibited greater sensitivity than did the gills and hepatopancreas. Further investigation found that K. mikimotoi increased the oxygen consumption rate in abalone and that hypoxia enhanced the hemolytic toxicity of K. mikimotoi. These results revealed that hypoxia and typical toxic algae cause synergistic harm to cultured organisms, which is expected to provide a new understanding of the destructive mechanisms of typical toxic algal blooms in aquacultural areas.</p

Additional file 1 of Genes expressed at low levels raise false discovery rates in RNA samples contaminated with genomic DNA

Additional file 1

Additional file 2 of Genes expressed at low levels raise false discovery rates in RNA samples contaminated with genomic DNA

Additional file 2

Additional file 3 of Genes expressed at low levels raise false discovery rates in RNA samples contaminated with genomic DNA

Additional file 3

Table_2_Knowledge, attitude, and practice toward tuberculosis prevention and management among household contacts in Suzhou Hospital, Jiangsu province, China.docx

BackgroundChina is among the 10 high-burden tuberculosis (TB) countries in the world; thus, investigation and management of household contacts is an essential part of TB prevention strategy.ObjectiveTo explore the knowledge, attitude, and practice (KAP) toward TB prevention and management among household contacts of TB patients.MethodsThis cross-sectional study enrolled household contacts in Suzhou Hospital of Integrated Traditional Chinese and Western Medicine between September 2022 and January 2023; KAP and demographic characteristics were assessed with the self-designed questionnaire and analyzed by multivariate logistic regression.ResultsA total of 503 participants were included; of them, 280 (55.78%) were female, and 303, (60.36%) aged ≥45 years. The KAP scores were 6.24 ± 2.20 (possible range: 0–12), 18.69 ± 2.80 (possible range: 0–36), and 20.37 ± 5.15 (possible range: 0–36), respectively. Suburban (OR = 0.18, 95% CI: 0.04–0.79, p = 0.023) and rural (OR = 0.12, 95% CI: 0.03–0.57, p = 0.008) were independently associated with knowledge. Positive attitude (OR = 7.03, 95% CI: 2.92–16.96, p ConclusionHousehold contacts of TB patients demonstrated insufficient knowledge, unfavorable attitude, and suboptimal practice toward TB prevention and management. Tailored interventions are needed to ensure information accessibility, especially for individuals living in suburban and rural areas.</p

Table_1_Knowledge, attitude, and practice toward tuberculosis prevention and management among household contacts in Suzhou Hospital, Jiangsu province, China.DOCX

BackgroundChina is among the 10 high-burden tuberculosis (TB) countries in the world; thus, investigation and management of household contacts is an essential part of TB prevention strategy.ObjectiveTo explore the knowledge, attitude, and practice (KAP) toward TB prevention and management among household contacts of TB patients.MethodsThis cross-sectional study enrolled household contacts in Suzhou Hospital of Integrated Traditional Chinese and Western Medicine between September 2022 and January 2023; KAP and demographic characteristics were assessed with the self-designed questionnaire and analyzed by multivariate logistic regression.ResultsA total of 503 participants were included; of them, 280 (55.78%) were female, and 303, (60.36%) aged ≥45 years. The KAP scores were 6.24 ± 2.20 (possible range: 0–12), 18.69 ± 2.80 (possible range: 0–36), and 20.37 ± 5.15 (possible range: 0–36), respectively. Suburban (OR = 0.18, 95% CI: 0.04–0.79, p = 0.023) and rural (OR = 0.12, 95% CI: 0.03–0.57, p = 0.008) were independently associated with knowledge. Positive attitude (OR = 7.03, 95% CI: 2.92–16.96, p ConclusionHousehold contacts of TB patients demonstrated insufficient knowledge, unfavorable attitude, and suboptimal practice toward TB prevention and management. Tailored interventions are needed to ensure information accessibility, especially for individuals living in suburban and rural areas.</p

Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation

Cell lines are applied on a large scale in the field of biomedicine, but they are susceptible to issues such as misidentification and cross-contamination. This situation is becoming worse over time due to the rapid growth of the biomedical field, and thus there is an urgent need for a more effective strategy to address the problem. As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules. The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities. Through flow cytometry measurements, the specific signals of each cell sample can be recorded and turned into a map divided into different regions by statistical processing. Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types. Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved. Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable

Additional file 1 of LncRNA SNHG1: role in tumorigenesis of multiple human cancers

Supplementary Material

Polyelectrolyte Multilayers Assembled Entirely from Immune Signals on Gold Nanoparticle Templates Promote Antigen-Specific T Cell Response

Materials that allow modular, defined assembly of immune signals could support a new generation of rationally designed vaccines that promote tunable immune responses. Toward this goal, we have developed the first polyelectrolyte multilayer (PEM) coatings built entirely from immune signals. These immune-PEMs (iPEMs) are self-assembled on gold nanoparticle templates through stepwise electrostatic interactions between peptide antigen and polyanionic toll-like receptor (TLR) agonists that serve as molecular adjuvants. iPEMs do not require solvents or mixing, offer direct control over the composition and loading of vaccine components, and can be coated on substrates at any scale. These films also do not require other structural components, eliminating the potentially confounding effects caused by the inherent immune-stimulatory characteristics of many synthetic polymers. iPEM loading on gold nanoparticle substrates is tunable, and cryoTEM reveals iPEM shells coated on gold cores. These nanoparticles are efficiently internalized by primary dendritic cells (DCs), resulting in activation, selective triggering of TLR signaling, and presentation of the antigens used to assemble iPEMs. In coculture, iPEMs drive antigen-specific T cell proliferation and effector cytokines but not cytokines associated with more generalized inflammation. Compared to mice treated with soluble antigen and adjuvant, iPEM immunization promotes high levels of antigen-specific CD8⁺ T cells in peripheral blood after 1 week. These enhancements result from increased DC activation and antigen presentation in draining lymph nodes. iPEM-immunized mice also exhibit a potent recall response after boosting, supporting the potential of iPEMs for designing well-defined vaccine coatings that provide high cargo density and eliminate synthetic film components

Hyperspectral imaging-based early damage degree representation of apple: a method of correlation coefficient

Hyperspectral imaging-based early damage degree representation of apple: a method of correlation coefficien