\u27What They Seek for is in Themselves\u27: Quaker Language and Thought in Eighteenth- and Nineteenth-Century American Literature

This paper argues that Quakerism was an important influence on a number of eighteenth- and nineteenth-century American writers. Looking at the work of, amongst others, Charles Brockden Brown, Robert Montgomery Bird, Ralph Waldo Emerson and John Greenleaf Whittier, it demonstrates that both the stereotyped depiction of Quakers and the use of Quaker ideas, such as the inward light in literature of the period, helped writers tackle some of the paradoxes of democracy in a young nation. The perceived mystery of Quaker individualism is used in these texts first to dramatize anxiety over the formation of American \u27character\u27 as either fundamentally unique and unknowable or representative of the whole nation, and secondly for more constructive ends in order to create a language able to express unity in diversity

Using competition assays to quantitatively model cooperative binding by transcription factors and other ligands.

BACKGROUND: The affinities of DNA binding proteins for target sites can be used to model the regulation of gene expression. These proteins can bind to DNA cooperatively, strongly impacting their affinity and specificity. However, current methods for measuring cooperativity do not provide the means to accurately predict binding behavior over a wide range of concentrations. METHODS: We use standard computational and mathematical methods, and develop novel methods as described in Results. RESULTS: We explore some complexities of cooperative binding, and develop an improved method for relating in vitro measurements to in vivo function, based on ternary complex formation. We derive expressions for the equilibria among the various complexes, and explore the limitations of binding experiments that model the system using a single parameter. We describe how to use single-ligand binding and ternary complex formation in tandem to determine parameters that have thermodynamic relevance. We develop an improved method for finding both single-ligand dissociation constants and concentrations simultaneously. We show how the cooperativity factor can be found when only one of the single-ligand dissociation constants can be measured. CONCLUSIONS: The methods that we develop constitute an optimized approach to accurately model cooperative binding. GENERAL SIGNIFICANCE: The expressions and methods we develop for modeling and analyzing DNA binding and cooperativity are applicable to most cases where multiple ligands bind to distinct sites on a common substrate. The parameters determined using these methods can be fed into models of higher-order cooperativity to increase their predictive power

Synthetic stellar populations: single stellar populations, stellar interior models and primordial proto-galaxies

We present a new set of stellar interior and synthesis models for predicting the integrated emission from stellar populations in star clusters and galaxies of arbitrary age and metallicity. This work differs from existing spectral synthesis codes in a number of important ways, namely (1) the incorporation of new stellar evolutionary tracks, with sufficient resolution in mass to sample rapid stages of stellar evolution; (2) a physically consistent treatment of evolution in the HR diagram, including the approach to the main sequence and the effects of mass loss on the giant and horizontal-branch phases. Unlike several existing models, ours yield consistent ages when used to date a coeval stellar population from a wide range of spectral features and colour indexes. We rigorously discuss degeneracies in the age-metallicity plane and show that inclusion of spectral features blueward of 4500 AA, suffices to break any remaining degeneracy and that with moderate S/N spectra (10 per 20AA, resolution element) age and metallicity are not degenerate. We also study sources of systematic errors in deriving the age of a single stellar population and conclude that they are not larger than 10-15%. We illustrate the use of single stellar populations by predicting the colors of primordial proto-galaxies and show that one can first find them and then deduce the form of the IMF for the early generation of stars in the universe. Finally, we provide accurate analytic fitting formulas for ultra fast computation of colors of single stellar populations. The models can be found at http://www.physics.upenn.edu/~rauljComment: MNRAS in pres

FINANCIAL PROSPECTS, BUSINESS ORGANIZATION, AND MANAGEMENT: FARM BUSINESS CHALLENGES

Farm Management,

Premature dismissal of high-redshift elliptical galaxies

It has recently been argued that single-collapse high-redshift models for elliptical galaxy formation can be rejected because they predict large numbers of very red galaxies at intermediate redshifts which are not seen in deep optical-infrared surveys. We argue, however, that this conclusion is premature since, while much effort has been invested in refining the predictions of hierarchical CDM models, only very simplistic models have been used to study the evolution of galaxies in other cosmogonies (e.g. isocurvature models). We demonstrate that the use of a more realistic multi-zone chemo-dynamical single-collapse model, yields colours at intermediate redshifts which are much bluer than inferred from the one-zone model, and indeed are comparable to those predicted by hierarchical merging despite still allowing $> 90$ of the final stellar mass of elliptical galaxies to be formed in the first Gyr of their evolution. We, therefore, conclude that the one-zone model should be avoided to predict the colours of high-redshift galaxies and that the use of realistic multi-zone models allows the existence of ellipticals at high redshift, being their dismissal premature.Comment: Submitted to MNRA

EVA Performance Prediction

Astronaut physical performance capabilities in micro gravity EV A or on planetary surfaces when encumbered by a life support suit and debilitated by a long exposure to micro gravity will be less than unencumbered pre flight capabilities. The big question addressed by human factors engineers is: what can the astronaut be expected to do on EVA or when we arrive at a planetary surface? A second question is: what aids to performance will be needed to enhance the human physical capability? These questions are important for a number of reasons. First it is necessary to carry out accurate planning of human physical demands to ensure that time and energy critical tasks can be carried out with confidence. Second it is important that the crew members (and their ground or planetary base monitors) have a realistic picture of their own capabilities, as excessive fatigue can lead to catastrophic failure. Third it is important to design appropriate equipment to enhance human sensory capabilities, locomotion, materials handling and manipulation. The evidence from physiological research points to musculoskeletal, cardiovascular and neurovestibular degradation during long duration exposure to micro gravity . The evidence from the biomechanics laboratory (and the Neutral Buoyancy Laboratory) points to a reduction in range of motion, strength and stamina when encumbered by a pressurized suit. The evidence from a long history of EVAs is that crewmembers are indeed restricted in their physical capabilities. There is a wealth of evidence in the literature on the causes and effects of degraded human performance in the laboratory, in sports and athletics, in industry and in other physically demanding jobs. One approach to this challenge is through biomechanical and performance modeling. Such models must be based on thorough task analysis, reliable human performance data from controlled studies, and functional extrapolations validated in analog contexts. The task analyses currently carried out for EVA activities are based more on extensive domain experience than any formal analytic structure. Conversely, physical task analysis for industrial and structured evidence from training and EV A contexts. Again on earth there is considerable evidence of human performance degradation due to encumbrance and fatigue. These industrial models generally take the form of a discounting equation. The development of performance estimates for space operations, such as timeline predictions for EVA is generally based on specific input from training activity, for example in the NBL or KC135. uniformed services tasks on earth are much more formalized. Human performance data in the space context has two sources: first there is the micro analysis of performance in structured tasks by the space physiology community and second there is the less structured evidence from training and EV A contexts

Flat-top temperature tuning response in periodically-poled nonlinear crystals

Second harmonic generation via periodically-poled nonlinear materials offers an efficient means of generating high-quality visible light that would be otherwise unattainable with traditional laser sources. While this technology has the potential for implementation in many mass-industrial applications, temperature stability requirements of 0.1 deg.C can make packaging with a pump source problematic. Using our high fidelity poling technique we have achieved precise placement of poled domains in Lithium Niobate based on the resulting mathematical models. These initial devices provide more than 4 deg.C flat-top temperature stability, albeit with a corresponding loss in operational efficiency. Our aim is to implement improved designs in magnesium-doped Lithium Niobate for packaging with near-room temperature diode-based pump sources, as could be applied towards RGB TV and projector applications

Jonathan Lethem's Genre Evolutions

The ideas I expound here proceed from an initial, rather broad observation that all of Jonathan Lethem's novels subvert established fictional genres in some way. For example, The Fortress of Solitudedisrupts a semi-autobiographical coming-of-age narrative with elements of fantasy and comic book super-heroics. As She Climbed Across the Table is billed as a "campus comedy," yet allows science fiction to infiltrate its witty satire on academic life. Girl in Landscape is a western set in space. Now, it can of course be argued that any genre is necessarily an unstable category, a somewhat volatile mixture of repeated, conventional elements and the variations that provide an individual text with a sense of identity. Scholars such as Margaret Cohen, in "Traveling Genre," have argued just this, and I take it as axiomatic throughout