Meningococcal purine and pyrimidine biosynthesis pathway deduced from KEGG pathway database [<b>49</b>].

<p>Genes found to be up-regulated are indicated (up). * indicates that PurB catalyzes conversion of SAICAR to IMP and IMP to AMP.</p

Principal component analysis (PCA) of meningococcal transcriptomes obtained at different blood co-cultivation time points.

<p>The numbers in the plot represent the relative positions of samples and co-cultivation time points. Dashed arrows represent the passage of time.</p

Differentially expressed meningococcal genes encoding stress response proteins during blood co-cultivation compared with T = 0min samples (log₂ ratio, p<0.01 with FDR).

<p>Differentially expressed meningococcal genes encoding stress response proteins during blood co-cultivation compared with T = 0min samples (log₂ ratio, p<0.01 with FDR).</p

Expression levels of ribosomal protein genes at different co-cultivation time points (relative to T = 0min).

<p>Each differently coloured line represents one gene (identified in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039718#pone.0039718.s004" target="_blank">Table S3</a>).</p

Measurements of blood components sampled at time points during blood and <i>N. meningitidis</i> co-cultivation.

1<p>Bactericidal activities are defined by reduction of CFU, shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039718#pone-0039718-g001" target="_blank">Figure 1A and C</a>.</p>2<p>A+C: Blood from donor A with addition of plasma from donor C to 10%.</p><p>n/a: not applicable; nd: not determined.</p

Detection of NMB0390 on the surface of meningococci by FACS.

<p>Meningococcal strain MC58 were recovered from human blood (donor A) after 0, 90 and 240 min of cultivation.</p

Schematic representation of the number of differentially regulated meningococcal genes.

<p>Genes were identified in blood co-cultivation experiments at different time points, which are indicated by colour keys. Genes were classified into TIGR functional categories. Minus signs indicate down-regulated gene number.</p

Comparison of gene expression detected by microarray analysis and using real-time RT-PCR (TaqMan).

<p>RT-PCR results were obtained using independent blood samples from donor A (TaqMan A) and donor B (TaqMan B) for co-cultivation experiments. Error bars were calculated from three independent biological replicates.</p

Comparison of amino acid sequence between NMB0390 (752 aa) and its homologues from meningococcal isolates.

1<p>Meningococcal strain information is available at <a href="http://pubmlst.org/neisseria" target="_blank">http://pubmlst.org/neisseria</a>.</p>2<p>From these meningococcal isolates DNA sequences were determined by sequencing PCR-generated NMB0390 homologues in present study.</p>3<p>Amino acid sequence were obtained either from deducing DNA sequences (noted above) or from NCBI and references <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039718#pone.0039718-Budroni1" target="_blank">[50]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039718#pone.0039718-Joseph1" target="_blank">[51]</a>.</p

Differentially expressed meningococcal genes encoding virulence factors^* during blood co-cultivation compared with T = 0min samples (log₂ ratio, p<0.01 with FDR).

*<p>as listed by Tettelin <i>et al</i>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039718#pone.0039718-Tettelin1" target="_blank">[6]</a>.</p