ATP production and mitochondrial potential analyses of lal−/− bone marrow MDSCs.

<p><b>A</b>). ATP concentrations in Ly6G⁺ MDSCs from the bone marrow of <i>lal+/+</i> mice and <i>lal−/−</i> mice. Results are means of 4 independent experiments. n = 3, **, p<0.01, *, p<0.5; <b>B</b>). Mitochondrial membrane potential of CD11b⁺Ly6G⁺ MDSCs from the bone marrow of <i>lal+/+</i> mice and <i>lal−/−</i> mice was analyzed by JC-1 staining. Positive staining of JC-1 red represents healthy mitochondria. Transition from negative staining of JC-1 red to positive staining of JC-1 green represents impaired mitochondrial potential.</p

Decreases of monomeric low-molecular-weight Ras GTPase superfamily in <i>lal−/−</i> bone marrow MDSCs.

<p>Decreases of monomeric low-molecular-weight Ras GTPase superfamily in <i>lal−/−</i> bone marrow MDSCs.</p

Increases of additional monomeric low-molecular-weight Ras GTPase superfamily in <i>lal−/−</i> bone marrow MDSCs.

<p>Increases of additional monomeric low-molecular-weight Ras GTPase superfamily in <i>lal−/−</i> bone marrow MDSCs.</p

Up-regulation of cyclin proteins in <i>lal−/−</i> bone marrow MDSCs.

<p>Up-regulation of cyclin proteins in <i>lal−/−</i> bone marrow MDSCs.</p

Decreases of large G-protein superfamily in <i>lal−/−</i> bone marrow MDSCs.

<p>Decreases of large G-protein superfamily in <i>lal−/−</i> bone marrow MDSCs.</p

Up-regulation of cytochrome and ATP synthesis subunits in <i>lal−/−</i> bone marrow MDSCs.

<p>Up-regulation of cytochrome and ATP synthesis subunits in <i>lal−/−</i> bone marrow MDSCs.</p

Affymetrix GeneChip microarray and Ingenuity Pathway analyses of lal−/− MDSCs.

<p><b>A</b>). Affymetrix GeneChip microarray analysis of Ly6G⁺ MDSCs from the bone marrow of <i>lal+/+</i> mice and lal−/− mice. Numbers represent percentages of changed genes in each category vs total changed genes between <i>lal−/−</i> mice and <i>lal+/+</i> mice; <b>B</b>). Differential gene expression of Ly6G⁺ MDSCs from the bone marrow of <i>lal+/+</i> mice and <i>lal−/−</i> mice was analyzed by Ingenuity Pathway Analysis. Changed genes in the PPARγ pathway, cholesterol biosynthesis and mTOR signaling pathway are presented; <b>C</b>) Flow cytometry of mTOR downstream effectors S6 and 4E-BP1 in Ly6G⁺ MDSCs from the bone marrow of <i>lal+/+</i> mice and <i>lal−/−</i> mice. n = 4.</p

Gene expression changes of centromere protein and chromosome structural genes in <i>lal−/−</i> bone marrow MDSCs.

<p>Gene expression changes of centromere protein and chromosome structural genes in <i>lal−/−</i> bone marrow MDSCs.</p

Up-regulation of histone cluster genes in <i>lal−/−</i> bone marrow MDSCs.

<p>Up-regulation of histone cluster genes in <i>lal−/−</i> bone marrow MDSCs.</p

ROS Pathway Analysis of lal−/− bone marrow MDSCs.

<p><b>A</b>). Differential gene expression of CD11b⁺Ly6G⁺ MDSCs from the bone marrow of <i>lal+/+</i> mice and <i>lal−/−</i> mice was analyzed by Ingenuity Pathway Analysis. Changed genes in the nitric oxide and reactive oxygen species production genes are presented; <b>B</b>). ROS production in CD11b⁺Ly6G⁺ MDSCs from the bone marrow of <i>lal+/+</i> mice and <i>lal−/−</i> mice. The ROS signal was statistically analyzed by mean fluorescent intensity (MFI). Results are means of 4 independent FACS experiments. n = 4, **, p<0.01.</p