Additional file 1: of Rapid phenotyping of crop root systems in undisturbed field soils using X-ray computed tomography

MatLab 8.0 script for noise elimination, provided as an .exe-file working on 16-bit unsigned integer

Plant Image Segmentation: Reference Images Wheat

This is a collection of segmentation reference images for the validation of image segmentation accuracy in plant phenotyping research. This collection contains 40 original wheat plant images taken in the field, and their hand-segmented resulting images using Photoshop, i.e. 80 image files in this share. The original images are identified with unique filenames, and the reference images are identified accordingly with the suffix "_Hand" at the end of filenames. <br><br>This collection will be further developed in order to include more phenotyping images of diverse plant species. Therefore, contribution or suggestion to this collection is highly appreciated.<br

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Additional file 8. Increase in crown root length of 24 genotypes under low nitrogen after the start of the split root treatment

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Additional file 6. Correlation coefficients (Pearson’s r) of traits. Correlation was done based on best linear unbiased estimates (BLUPS). Abbreviations: Number of lateral roots in the first segment (NoLat 1st) or second segment (NoLat 2nd), length of representative lateral root in the first segment (MedLat 1st) or second segment (MedLat 2nd), maximal lateral root length in the first segment (MaxLat 1st) or in the second segment (MaxLat 2nd), branching density in the first segment (BrLat1st) or in the second segment (BrLat2nd), branching density across both segments (BrLatTot), length of the branching zone (LBrZone), total number of lateral roots (NoLatTot), elongation rate crown roots (ERCr), length of crown roots at solution change (intercept; ICCr), embryonic root dry weight (DWER), crown root dry weight (DWCR), leaf greenness (SPAD), leaf area measured (LAm), dry weight shoot (DWS), leaf area pixel based (LAPix), shoot pixel count at solution change intercept (ICS), shoot pixel count development (ERS), N content in the leaf in % of total dry weight (N). Significance level: ≤ 0.001 ***, ≤ 0.01**, ≤ 0.05*

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Additional file 7. Increase in crown root length of 24 genotypes under high nitrogen after the start of the split root treatment

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Additional file 5. A) Best linear unbiased prediction (BLUPS) of parameters with significant treatment effect, but no genotype effect or genotype: treatment interaction. B) Prediction of mean values of parameters with significant genotype effect, but no genotype: treatment interaction. C) Prediction of mean values of parameters with significant genotype: treatment interaction. Abbreviations: Number of lateral roots in the first segment (NoLat 1st) or second segment (NoLat 2nd), length of representative lateral root in the first segment (MedLat 1st) or second segment (MedLat 2nd), maximal lateral root length in the first segment (MaxLat 1st) or in the second segment (MaxLat 2nd), elongation rate crown roots (ERCr), length of crown roots at solution change (intercept; ICCr), embryonic root dry weight (DWER), crown root dry weight (DWCR), chlorophyll measurements (SPAD, measured leaf area (LAm), shoot dry weight (DWS), number of pixels specifying the leaf area (LAPix), shoot pixel count at solution change (intercept; ICS), shoot pixel count development (ERS), N content in the leaf in % of total dry weight (N in %)

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Additional file 10. Increase of lateral roots after solution change above the last formed lateral

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Additional file 4. Shoot growth of 24 different genotypes quantified by the increase in number of pixels after the start of the split root treatment

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Additional file 9. Residual vs. fitted crown root length of multiple linear models to determine intercept and slope of crown root development after solution change

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