Phylogenetic analysis of <i>B oleae</i> putative CCEs.

<p><i>B. oleae</i> CCEs clustered within clades <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Claudianos1" target="_blank">[36]</a>. A: hymenopteran radiation with related group containing odorant degrading esterases, B: dipteran mitochondrial, cytosolic and secreted esterases, C: dipteran microsomal, α-esterases, D: integument esterases, E: β-esterases and pheromone esterases, F: dipteran juvenile hormone esterases, G: lepidopteran juvenile hormone esterases, H: glutactin and glutactin-like enzymes, I: uncharacterized group, J: acetylcholinesterases, K: gliotactins, L: neuroligins, M: neurotactins. Aaeg: <i>Aedes aegypti</i>, Acal: <i>Anisopteromalus calandrae</i>, Agam: <i>Anopheles gambiae</i>, Agos: <i>Aphis gossypii</i>, Amel: <i>Apis mellifera</i>, Apol: <i>Antheraea polyphemus</i>, Bdor: <i>Bactrocera dorsalis</i>, Bmor: <i>Bombyx mori</i>, Bole: <i>Bactrocera oleae</i>, Cfum: <i>Choristoneura fumiferana</i>, Cpip: <i>Culex pipiens</i>, Ctar: <i>Culex tarsalis</i>, Ctri: <i>Culex tritaeniorhynchus</i>, Dmel: <i>Drosophila melanogaster</i>, Hirr: <i>Haematobia irritans</i>, Hvir: <i>Heliothis virescens</i>, Lcup: <i>Lucilia cuprina</i>, Ldec: <i>Leptinotarsa decemlineata</i>, Mdom: <i>Musca domestica</i>, Mper: <i>Myzus persicae,</i> Nlug: <i>Nilaparvata lugens</i>, Sgra: <i>Schizaphis graminum</i>, Tcas: <i>Tribolium castaneum,</i> Tmol: <i>Tenebrio molitor</i>.</p

Enzyme Classification (EC) analysis of the transcriptome of <b><i>B. oleae.</i></b>

<p>(A) Distribution of EC number in general EC terms, (B) percentage of EC number distribution of <i>B. oleae</i> compared to that from transcriptome sequencing of <i>B. dorsali</i>s <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Hsu2" target="_blank">[14]</a>, <i>M. domestica </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Liu1" target="_blank">[16]</a> and <i>T. vaporariorum </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Karatolos1" target="_blank">[15]</a>.</p

Summary of run statistics and assembly.

*<p>size above which 50% of the assembled sequences can be found.</p

Comparison of ABC genes in different insect species.^*

*<p>numbers were derived from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Sturm1" target="_blank">[42]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Liu2" target="_blank">[43]</a> and this study.</p

Comparison of CCEs in different insect species.^*

*<p>numbers were derived from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Hsu2" target="_blank">[14]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Karatolos1" target="_blank">[15]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Hayes1" target="_blank">[40]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Oakeshott2" target="_blank">[41]</a> and this study.</p>**<p>for full CCE clade names, see legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone-0066533-g006" target="_blank">Figure 6</a>.</p

Phylogenetic analysis of <b><i>B. oleae</i></b>putative P450s.

<p><i>B. oleae</i> P450s clustered within the 4 major insect CYP clades. Aech: <i>Acromyrmex echinatior,</i> Agam: <i>Anopheles gambiae</i>, Amel: <i>Apis mellifera,</i> Bdor: <i>Bactrocera dorsalis</i> (“JF” sequences were obtained from Shen <i>et al</i>., 2011), Bger: <i>Blatella germanica,</i> Bmor: <i>Bombyx mor</i>i, Btab: <i>Bemisia tabaci</i>, Dmel: <i>Drosophila melanogaster,</i> Dpas: <i>Depressaria pastinacella</i>, Dpun<i>: Diploptera punctata</i>, Harm: <i>Helicoverpa armigera</i>, Hsap<i>: Homo sapiens</i>, Hzea: <i>Helicoverpa zea</i>, Mdom: <i>Musca domestica</i>, Ppol: <i>Papilio polyxenes</i>.</p

Comparison of P450s in different insect species.^*

*<p>numbers were derived from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Hsu2" target="_blank">[14]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Karatolos1" target="_blank">[15]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066533#pone.0066533-Feyereisen1" target="_blank">[19]</a> and this study.</p

Functional annotation clusters in the up-regulated genes (analyzed with the DAVID 6.7 BETA bioinformatics resource).

<p>Functional annotation clusters in the up-regulated genes (analyzed with the DAVID 6.7 BETA bioinformatics resource).</p

Crossing scheme of the genome-wide insertional mutagenesis system.

<p>TREP 2.30 – promoter delivery, minimal promoter under tTA control, <i>w⁺</i> marker <i>CyO</i> [MiT 2.4] – <i>Minos</i> transposase source, CyO marker <i>Sco</i> – Sco marker BOEtTA – tTA source, <i>egfp</i> marker.</p

Gene functional groups in the up-regulated genes (analyzed with the DAVID 6.7 BETA bioinformatics resource).

*<p>Kappa score – The Kappa value quantitatively measures the degree to which genes share similar annotation terms (the higher the Kappa, the stronger the functional similarity).</p