Additional file 5: of Quorum sensing in thermophiles: prevalence of autoinducer-2 system

Multiple sequence alignment of ComA protein from Bacillus subtilis and thermophilic eubacteria by MultAlin. (PDF 120 kb

Additional file 3: of Quorum sensing in thermophiles: prevalence of autoinducer-2 system

Multiple sequence alignment of AgrA protein from Staphylococcus aureus and thermophilic eubacteria by MultAlin. (PDF 40 kb

Additional file 15 of Quorum sensing in thermophiles: prevalence of autoinducer-2 system

STRING analysis of LuxS protein of Caminibacter mediatlanticus. (PDF 90 kb

N-acyl homoserine lactone mediated interspecies interactions between <i>A. baumannii</i> and <i>P. aeruginosa</i>

<div><p> <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> are pathogens capable of colonizing the same infection sites and employing N-acyl homoserine lactone (AHL) based quorum-sensing systems to co-ordinate biofilm formation. Hence, the effect of <i>P. aeruginosa</i> AHLs on biofilm formation by <i>A. baumannii</i> and <i>vice versa</i> were investigated using the biofilm impaired quorum sensing mutants, <i>A. baumannii</i> M2 (<i>aba</i>I::Km) and <i>P. aeruginosa</i> PAO-JP2. Complementing the mutants with heterologous, extracted and pure AHLs increased biofilm mass significantly. The surface area coverage and biovolume also increased significantly as observed by confocal scanning laser microscopy which corroborated scanning electron microscope analysis. Autoinducer synthase gene promoters of <i>A. baumannii</i>, P_<i>aba</i>I-<i>lac</i>Z, and <i>P. aeruginosa</i>, P_<i>las</i>I-<i>lacZ</i>, were induced (<i>p</i> < 0.05) by heterologous AHLs. Growth of <i>A. baumannii</i> was not inhibited by pyocyanin of <i>P. aeruginosa</i> which may allow their co-existence and interaction in the clinical setting, thereby affecting the severity of combined infections and therapeutic measures to control them.</p> </div

Additional file 14: of Quorum sensing in thermophiles: prevalence of autoinducer-2 system

STRING analysis of LuxS protein of Geobacillus thermoglucosidasius. (PDF 225 kb

Additional file 9: of Quorum sensing in thermophiles: prevalence of autoinducer-2 system

Multiple sequence alignment of SAH hydrolase from thermophilic and mesophilic eubacteria by MultAlin. High conservation among SAH hydrolases has been observed. (PDF 274 kb

Additional file 12: of Quorum sensing in thermophiles: prevalence of autoinducer-2 system

Multiple sequence alignment of RbsB protein from mesophilic and thermophilic eubacteria by MultAlin. Conserved residues are highlighted in red within boxes. (PDF 142 kb

Additional file 6: of Quorum sensing in thermophiles: prevalence of autoinducer-2 system

Multiple sequence alignment of LuxS protein from mesophilic and thermophilic eubacteria by MultAlin. Invariant residues are highlighted in red colour. The conserved HTLEH motif and other conserved residues are highlighted within boxes. (PDF 111 kb

Relative expression of <i>lasIR</i> and <i>rhlIR</i> genes of <i>P. aeruginosa</i> PAO1 in the presence of 0.5 mg/ml F7 as determined by qRT PCR.

<p>Relative expression of <i>lasIR</i> and <i>rhlIR</i> genes of <i>P. aeruginosa</i> PAO1 in the presence of 0.5 mg/ml F7 as determined by qRT PCR.</p

Additional file 1: of Quorum sensing in thermophiles: prevalence of autoinducer-2 system

Peptide based quorum sensing systems. (PDF 10 kb