sj-pdf-1-pus-10.1177_09636625231225073 – Supplemental material for The effects of self-disclosure and gender on a climate scientist’s credibility and likability on social media

Supplemental material, sj-pdf-1-pus-10.1177_09636625231225073 for The effects of self-disclosure and gender on a climate scientist’s credibility and likability on social media by Nahyun Kim, Chris Skurka and Stephanie Madden in Public Understanding of Science</p

Supplemental Material - The Role of Late-Life Work Among Widowed Adults in Korea: A Buffer or Burden for Widow(er)s’ Health?

Supplemental Material for The Role of Late-Life Work Among Widowed Adults in Korea: A Buffer or Burden for Widow(er)s’ Health? by Hyeonji Cho, Nahyun Kim, and Kyungmin Kim in Original Manuscript</p

Effect of oleuropein on hair follicle growth in C57BL/6N mice.

<p>The CON, 0.4 mg/mL OP, or 3 mg/mL MXD was topically applied to the shaved back skin of C57BL/6N mice for 28 days. (A) The effect of oleuropein on the hair follicles of the mice was analyzed by using hematoxylin and eosin staining. Longitudinal sections of the back skin were stained, and representative photomicrographs of skin sections are shown. Bars, 200 μm. Number (B) and diameter (C) of hair follicles in deep subcutis. Values are the mean ± SD of eight mice. * = <i>p</i> < 0.05, ** = <i>p</i> < 0.01, *** = <i>p</i> < 0.001 (one-way ANOVA, Tukey's test).</p

Effect of oleuropein on mRNA and protein expression of growth factors in C57BL/6N mouse skin tissue.

<p>(A) The IGF-1, HGF, VEGF, and KGF mRNA expression levels were measured by RT-PCR. A representative image of triplicate experiments is shown in the left panel. The right panel shows the intensity of the bands that were densitometrically measured and normalized against the mRNA expression level of GAPDH. (B) Dermal levels of IGF-1 were assessed by ELISA. (C) Immunohistochemical analysis of IGF-1 expression in hair follicle. Original magnification: 40×. The RT-PCR results are the mean (<i>n</i> = 8) ± SD of three independent experiments (<i>n</i> = 2 or 3 per experiment) for each group. ns = <i>p</i> > 0.05, * = <i>p</i> < 0.05, ** = <i>p</i> < 0.01, *** = <i>p</i> < 0.001 (one-way ANOVA, Tukey's test).</p

Effect of oleuropein on the activation status of the Wnt/β-catenin pathway in DPCs.

<p>DPCs were treated with 20 μM OP or 100 μM MXD for 24 h. (A) The protein level of β-catenin in the DPCs was determined by western blotting. A representative image of triplicate experiments is shown in the upper panel. The lower panel shows the intensity of the bands that were densitometrically measured and normalized against the protein level of β-actin. (B) DPCs were immunocytochemically stained with β-catenin antibody (left column panel), and the corresponding DAPI nuclear staining is also shown (middle column panel); merged images are shown in the right column panel. Original magnification: 20×. (C) LEF1 and Cyc-D1 mRNA levels were measured by RT-PCR. A representative image of triplicate experiments is shown in the left panel. The right panel shows the intensity of the bands that were densitometrically measured and normalized against the mRNA expression level of GAPDH. The western blotting and RT-PCR results are the mean ± SD from duplicates of three independent experiments. * = <i>p</i> < 0.05, ** = <i>p</i> < 0.01, *** = <i>p</i> < 0.001 (one-way ANOVA, Tukey's test).</p

Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.

<p>DPCs were allowed to attach overnight and then treated (60 min) with cycloheximide (10 μg/ml) and thereafter switched to medium with or without oleuroepin (20 μM) in the presence of cycloheximide (10 μg/ml) for 24 h. mRNA expression levels of LEF1, Cyc-D1, IGF-1, HGF, VEGF, and KGF were measured by RT-PCR. A representative image of triplicate experiments is shown in the left panel. The right panel shows the intensity of the bands that were densitometrically measured and normalized against the mRNA expression level of GAPDH. The RT-PCR results are the mean ± SD from duplicates of three independent experiments. ns = <i>P</i> > 0.05, * = <i>p</i> < 0.05, ** = <i>p</i> < 0.01 (student’s t-test).</p

Effect of oleuropein on expression of genes related to hair growth in C57BL/6N mice skin tissue.

<p>(A) The Wnt10b, DKK1, LRP5, FZDR1, LEF1, and Cyc-D1 mRNA expression levels were measured by RT-PCR. A representative image of triplicate experiments is shown in the left panel. The right panel shows the intensity of the bands that were densitometrically measured and normalized against the mRNA expression level of GAPDH. (B) The protein level of β-catenin in C57BL/6N mouse skin tissue was determined by western blotting. The lower panel shows the intensity of the bands that were densitometrically measured and normalized against the protein level of β-actin. (C) Immunohistochemical analysis of β-catenin in hair follicles. Original magnification: 40×. Western blotting and RT-PCR results are the mean (<i>n</i> = 8) ± SD of three independent experiments (<i>n</i> = 2 or 3 per experiment) for each group. ns = <i>p</i> > 0.05, * = <i>p</i> < 0.05, ** = <i>p</i> < 0.01, *** = <i>p</i> < 0.001 (one-way ANOVA, Tukey's test). </p

Effects of cycloheximide on β-catenin accumulation in the nucleus of DPCs induced by oleuropein treatment.

<p>DPCs were allowed to attach overnight and then treated (60 min) with cycloheximide (10 μg/ml) and thereafter switched to medium with or without oleuroepin (20 μM) in the presence of cycloheximide (10 μg/ml) for 24 h. Then cells were stained for β-catenin immunofluorescence (red) and counterstained with DAPI (blue). Merged image of β-catenin-rhodamine and DAPI staining is also shown. Original magnification: 20×.</p

Effect of oleuropein on DPC proliferation.

<p>DPCs (3 × 10⁴ cells/well) were treated with various concentrations of oleuropein (OP) or minoxidil (MXD), as indicated. (A) Viable cells were determined by MTT assay. (B) Cells were counted by trypan blue exclusion. The values are the mean ± SD of triplicate determinations from three independent wells. * = <i>P</i> < 0.05 and ** = <i>P</i> < 0.01 <i>vs</i>. control.</p

Awesome, Awful: Emotional Flow in Environmental Messaging

Pro-environmental media content, such as nature documentary programming, often features awe-inducing scenes of Earth’s natural beauty, and exposure to this kind of content has been shown to increase persuasive outcomes. Yet environmental messaging is increasingly likely to portray the tragic impacts of human activities on these natural wonders alongside content about Earth’s beauty. How might emotional responses evolve during exposure to sequenced messages that juxtapose positively-valenced (beauty) and negatively-valenced (negative impacts) pro-environmental content? Embracing an emotional flow perspective as our overarching lens, we put forth two accounts for how emotions might shift during sequenced messaging: contrast effects and the elicitation of poignancy. We tested these accounts in a between-subjects experiment with U.S. adults (N = 979), following a 5 (focus: beauty-only, impacts-only, beauty→impacts, impacts→beauty, or control) × 2 (topic: coral reefs or forests) design. We found no evidence for contrast effects in discrete emotion intensity (awe, hope, sadness, or fear). The sequenced messages evoked greater poignancy than the static messages, which in turn predicted greater intentions to share the message. Although the sequenced messages indirectly predicted sharing intentions and policy support via several other emotion-based mechanisms, these outcomes did not differ between the static and sequenced conditions.</p