Pengujian Ga3 Dan Rasio Tanam Tetua Terhadap Produksi Benih Hibrida Hipa 14 Melalui Rancangan Petak Terbagi

The three lines methods in hybrid rice have disadvantages in seed production procedures that result in low seed yield. Low levels of natural crosses (outcrossing) is one of the causes of low seed yield in hybrid seed production. GA3 application and proper spacing , expected to improve of F1 hybrid seed production. The experimental design used was Split Plot design with three replications. The main plot was doses of GA3 application consist of 0, 200 ppm and 300 ppm. The subplot was row ratios of restores:CMS i,e 2R:8A, 2R:12A; 2R:16A. The material used is parental lines of HIPA 14. The results showed that GA3 application was able to increased plant height of parental lines (CMS and restorer), number of productive tillers, exterted panicle and stigma also outcrossing rate at seed production of HIPA 14. The row ratio was influenced for plant heigh of CMS lines. Interaction between GA3 and row ratio was increased plant height of parental lines, exerted panicle, and outcrossing rat

Identifikasi Galur Tetua Padi Hibrida Dengan Marka SSR Spesifik Dan Pemanfaatannya Dalam Uji Kemurnian Benih

DNA finger printing of hybrid rice variety and their parental lines are important molecular traits as a tool in plant variety protection, DUS test, and seed purity assessment. Molecular markers including microsatellite (SSR) markers have been used in varietal assessment. This study was aimed to identify specific SSR markers that can be used to distinguish parent lines of hybrid rice and to assess seed purity. The experiment was conducted in 2011 at Biology Molecular Laboratory of ICABIOGRAD, Bogor. Sixteen SSR markers were used to identify parental lines of five hybrid varieties of rice. Two hybrid varieties (F1), Hipa 6 and Hipa 7, were used in the seed purity assessment. Seven markers were polymorphic, and six of them (RM206, RM263, RM276, RM346, RM335, RM570) were capable of distinguishing parental lines of the five hybrid varieties. RM 346 marker could be used for testing seed purity of Hipa 6, while RM 206 marker for Hipa 7. Specific SSR marker was capable of distinguishing a false hybrid rice that might not be recognized by the grow out test in the field