The relationship of 1,25-dihydroxyvitamin D and Vitamin D binding protein in periodontitis

Objective: This study was conducted to explore the relationship between 1,25-dihydroxy vitamin D (1,25(OH)2D) and Vitamin-D binding protein (DBP) in patients with periodontitis and healthy controls.&#x0D; Methods: Seventy-five periodontitis cases were recruited from the dental OPD of Dow University of Health Sciences, Karachi. Diagnostic criteria of periodontitis were followed according to the probe pocket depth and clinical attachment loss. Seventy-five periodontal healthy controls were selected from the faculty and students of same university. Serum levels of 1,25(OH)2D and DBP were determined by ELISA.&#x0D; Results: Significantly low levels of 1,25(OH)2D and high levels of serum DBP were observed in periodontitis patients compared to healthy controls (p&lt;0.05), with levels of DBP increasing significantly with the severity of periodontitis (p=0.005). Concentrations of DBP correlated positively with 1,25(OH)2D, especially in cases with periodontitis (r =0.780; p&lt;0.001).&#x0D; Conclusion: Within the limits of the study, we conclude that low 1,25(OH)2D levels and high DBP levels are associated with periodontitis.&#x0D; doi: https://doi.org/10.12669/pjms.35.3.482&#x0D; How to cite this:Rafique S, Hingorjo MR, Mumtaz M, Qureshi MA. The relationship of 1,25-dihydroxyvitamin D and Vitamin D binding protein in periodontitis. Pak J Med Sci. 2019;35(3):847-851. doi: https://doi.org/10.12669/pjms.35.3.482&#x0D; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</jats:p

Association of Resistin with Components of Metabolic Syndrome in Our Local Population

Objective: To investigate the association of resistin with components of metabolic syndrome (MetS) in our local population. Study design &amp; setting: Case-control study, Medical OPD; Lyari General Hospital Karachi (from 15th July 2020 till 15th December 2020). Methodology: A total of 164 subjects (83 cases &amp; 81 controls) between the age ranges of 35-65 years were selected. Subjects with metabolic syndrome were included in concordance with International Diabetes Federation criteria. Serum resistin levels in both cases and controls were compared. SPSS 20 was used for statistical analysis. Eighty-three patients with metabolic syndrome were enrolled and compared with 81 healthy unmatched controls. Obesity indices, blood pressure, lipid profile, fasting blood sugar, insulin resistance and serum resistin levels were evaluated. Predictive values of resistin for MetS were analyzed using odds ratios. Results: Significantly elevated levels of resistin were found in subjects with metabolic syndrome compared to healthy controls (8.23±4.43 vs 6.82±3.64 ng/mL, p&lt;0.05). The odds ratio (95% confidence interval) for metabolic syndrome in subjects having higher resistin were: males:2.62[1.11–6.19], females: 2.81[1.05–7.49], all Ptrend&lt;0.05.For individual components of metabolic syndrome, we found the odds ratio to be greatest for hypertension, fasting blood sugar, and insulin resistance; 2.64, 4.83, 2.85, respectively, all Ptrend&lt;0.05. Conclusion: The present study suggests significant association of resistin with components of metabolic syndrome such as hypertension, fasting blood sugar, and insulin resistance. Compared to healthy controls, subjects with MetS had significantly higher resistin levels. Further research is required to incorporate this biomarker in clinical setting.</jats:p

Role of C-reactive protein and gamma-glutamyl transferase in the diagnosis of metabolic syndrome

Previous studies have reported that metabolic syndrome (MetS) is associated with an increased risk of major cardiovascular events and levels of C-Reactive protein (CRP) can be considered as markers of MetS and its constituent components. Oxidative stress plays a major role in the development of MetS, and levels of gamma-glutamyl transferase (GGT) change with response to oxidative stress are also associated with MetS, which may be modulated by CRP. This study was conducted to identify the role of GGT and CRP as biomarkers in the diagnosis of MetS, a high-risk factor for cardiovascular diseases. One hundred and fifty patients meeting the diagnostic criteria of MetS and an equal number of controls were included in the study. The cases were selected from pathology and molecular biology laboratories, Karachi, while the controls came from the general population. Anthropometric indices of adiposity and blood pressure were recorded for both cases and controls. Blood samples were taken from all subjects to determine the levels of CRP and GGT. All those cases and control height, weight, hip waist circumference were noted and the comparison of CRP and GGT by applying students' t-test as markers for detection of metabolic syndrome. p-value 0.001 was considered as significant. This study suggests that in patients with metabolic syndrome were found to have raised the basal metabolic rate, C-reactive protein and GGT were synergistically associated with MetS independently of another confounding factor in the general population. Keywords: C-reactive protein (CRP), gama glutamyl transferase (GGT), metabolic syndrome, (Met-S), inflammation, body mass index.</jats:p