Targeted Discovery of Sorbicillinoid Pigments with Anti-Inflammatory Activity from the Sponge-Derived Fungus Stagonospora sp. SYSU-MS7888 Using the PMG Strategy

An effective identification and discovery of fungal pigments is very important to illustrate the role of fungal pigments in the life process and conduce to the discovery of new bioactive and edible pigments. The phenotype combined with metabolomic and genomic (PMG) strategy led to the discovery and characterization of three new sorbicillinoid pigments, stasorbicillinoids A–C (1–3), and five known analogues (4–8) from the sponge-derived fungus Stagonospora sp. SYSU-MS7888. Their structures were elucidated by the application of spectroscopic methods (NMR, MS, UV, IR, and ECD) and modified Mosher’s method. Compounds 1 and 2 featured novel naphthone nuclei linked by two alkyl side chains possibly undergoing inter- and intramolecular Michael reactions. Compounds 1–8 exhibited potent anti-inflammatory activity with IC50 values in the range of 3.56–22.8 μM. Furthermore, compound 2 inhibited the production of IL-1β, IL-6, and TNF-α in a dose-dependent manner. This study provides an effective strategy to accelerate the discovery of new fungal pigments and further exploration of their potential applications in different fields such as medicine and food industries

Shikimate-Derived Meroterpenoids from the Ascidian-Derived Fungus <i>Amphichorda felina</i> SYSU-MS7908 and Their Anti-Glioma Activity

Glioma is a clinically heterogeneous type of brain tumor with a poor prognosis. Current treatment approaches have limited effectiveness in treating glioma, highlighting the need for novel drugs. One approach is to explore marine natural products for their therapeutic potential. In this study, we isolated nine shikimate-derived diisoprenyl-cyclohexene/ane-type meroterpenoids (1–9), including four new ones, amphicordins A–D (1–4) from the ascidian-derived fungus Amphichorda felina SYSU-MS7908, and further semisynthesized four derivatives (10–13). Their structures were extensively characterized using 1D and 2D NMR, modified Mosher’s method, HR-ESIMS, NMR and ECD calculations, and X-ray crystallography. Notably, amphicordin C (3) possesses a unique benzo[g]chromene (6/6/6) skeleton in this meroterpenoid family. In an anti-glioma assay, oxirapentyn A (7) effectively inhibited the proliferation, migration, and invasion of glioma cells and induced their apoptosis. Furthermore, an in silico analysis suggested that oxirapentyn A has the potential to penetrate the blood–brain barrier. These findings highlight the potential of oxirapentyn A as a candidate for the development of novel anti-glioma drugs

Shikimate-Derived Meroterpenoids from the Ascidian-Derived Fungus <i>Amphichorda felina</i> SYSU-MS7908 and Their Anti-Glioma Activity

Glioma is a clinically heterogeneous type of brain tumor with a poor prognosis. Current treatment approaches have limited effectiveness in treating glioma, highlighting the need for novel drugs. One approach is to explore marine natural products for their therapeutic potential. In this study, we isolated nine shikimate-derived diisoprenyl-cyclohexene/ane-type meroterpenoids (1–9), including four new ones, amphicordins A–D (1–4) from the ascidian-derived fungus Amphichorda felina SYSU-MS7908, and further semisynthesized four derivatives (10–13). Their structures were extensively characterized using 1D and 2D NMR, modified Mosher’s method, HR-ESIMS, NMR and ECD calculations, and X-ray crystallography. Notably, amphicordin C (3) possesses a unique benzo[g]chromene (6/6/6) skeleton in this meroterpenoid family. In an anti-glioma assay, oxirapentyn A (7) effectively inhibited the proliferation, migration, and invasion of glioma cells and induced their apoptosis. Furthermore, an in silico analysis suggested that oxirapentyn A has the potential to penetrate the blood–brain barrier. These findings highlight the potential of oxirapentyn A as a candidate for the development of novel anti-glioma drugs

Shikimate-Derived Meroterpenoids from the Ascidian-Derived Fungus <i>Amphichorda felina</i> SYSU-MS7908 and Their Anti-Glioma Activity

Glioma is a clinically heterogeneous type of brain tumor with a poor prognosis. Current treatment approaches have limited effectiveness in treating glioma, highlighting the need for novel drugs. One approach is to explore marine natural products for their therapeutic potential. In this study, we isolated nine shikimate-derived diisoprenyl-cyclohexene/ane-type meroterpenoids (1–9), including four new ones, amphicordins A–D (1–4) from the ascidian-derived fungus Amphichorda felina SYSU-MS7908, and further semisynthesized four derivatives (10–13). Their structures were extensively characterized using 1D and 2D NMR, modified Mosher’s method, HR-ESIMS, NMR and ECD calculations, and X-ray crystallography. Notably, amphicordin C (3) possesses a unique benzo[g]chromene (6/6/6) skeleton in this meroterpenoid family. In an anti-glioma assay, oxirapentyn A (7) effectively inhibited the proliferation, migration, and invasion of glioma cells and induced their apoptosis. Furthermore, an in silico analysis suggested that oxirapentyn A has the potential to penetrate the blood–brain barrier. These findings highlight the potential of oxirapentyn A as a candidate for the development of novel anti-glioma drugs

Additional file 6 of Transcriptional landscape of circulating platelets from patients with COVID-19 reveals key subnetworks and regulators underlying SARS-CoV-2 infection: implications for immunothrombosis

Additional file 6: Table S2. Primers sequences for four candidate mRNAs and reference mRNA

Additional file 3 of Transcriptional landscape of circulating platelets from patients with COVID-19 reveals key subnetworks and regulators underlying SARS-CoV-2 infection: implications for immunothrombosis

Additional file 3: Figure S3. Functional characterization of key regulators in circulating platelets during SARS-CoV-2 infection. (A) Tissue and cell-specific enrichment analysis. (B) Disease enrichment analysis. (C) Enrichment analysis of differentially expressed RNAs in viral perturbations datasets from the Gene Expression Omnibus database. SARS-CoV-2, severe acute respiratory syndrome coronavirus 2

Additional file 2 of Transcriptional landscape of circulating platelets from patients with COVID-19 reveals key subnetworks and regulators underlying SARS-CoV-2 infection: implications for immunothrombosis

Additional file 2: Figure S2. Hierarchical clustering of transcriptome-wide RNA expression. Hierarchical clustering of samples according to global gene expression segregates SARS-CoV-2-infected patients (red numbers) from healthy donors (gray numbers). SARS-CoV-2, severe acute respiratory syndrome coronavirus 2

Additional file 1 of Transcriptional landscape of circulating platelets from patients with COVID-19 reveals key subnetworks and regulators underlying SARS-CoV-2 infection: implications for immunothrombosis

Additional file 1: Figure S1. The imaging of all patients

Additional file 5 of Transcriptional landscape of circulating platelets from patients with COVID-19 reveals key subnetworks and regulators underlying SARS-CoV-2 infection: implications for immunothrombosis

Additional file 5: Table S1. Lists of differentially expressed RNAs between COVID-19 patients and healthy donors

Additional file 4 of Transcriptional landscape of circulating platelets from patients with COVID-19 reveals key subnetworks and regulators underlying SARS-CoV-2 infection: implications for immunothrombosis

Additional file 4: Figure S4. Boxplots showing expression levels of four key regulators using RT-qPCR