Integrated miRNA-mRNA analysis.

The top 200 most negatively correlated putative miRNA-mRNA pairs (P < 0.05). Light blue circle indicates up-regulated miRNAs and red circle indicates down-regulated mRNAs. Light blue square indicates down-regulated miRNAs and red square indicates up-regulated mRNAs.</p

Putative negative miRNA-mRNA pairs validated by RT-qPCR (<i>P</i> < 0.05).

Putative negative miRNA-mRNA pairs validated by RT-qPCR (P < 0.05).</p

List of DE mature miRNAs in kidneys of CI-AKI rats.

List of DE mature miRNAs in kidneys of CI-AKI rats.</p

Histopathological assessment of the kidneys of the control rats and CI-AKI rats by H&E staining.

(A) CM treatment induces representative histopathological changes in the outer medulla of H&E-stained kidney sections. (B) Tubular injury is graded by the Paller score, a semi-quantitative evaluation. For each kidney section, 100 tubules from 10 highmagnification (×200) fields of the inner and outer medulla are scored. The average Paller score is much higher in CI-AKI rats than controls ((8.2 ± 0.9) vs. (3.6 ± 1.2), P < 0.05).</p

Baseline characteristics of experimental groups and the effects of contrast medium on changes in SCr elevation.

Baseline characteristics of experimental groups and the effects of contrast medium on changes in SCr elevation.</p

RT-qPCR validation.

(A) RT-qPCR validation of six miRNAs selected from the miRNA sequencing data. N = 6 per group, *P P < 0.05 vs. control group.</p

Hierarchical clustering analysis of the 453 DE mRNAs in the CI-AKI and control group (<i>P</i> < 0.05).

Each group includes 3 duplicates. Colors from green to red represent the gene expression abundance from poor to rich.</p

Functional enrichment analysis of the mature DE miRNAs and their putative targets.

(A) The enriched top 30 GO terms (P P < 0.05).</p

Hierarchical clustering analysis of the 72 DE miRNAs in the CI-AKI and control group.

Each group includes 3 duplicates. The DE miRNAs includes 36 mature and 36 novel ones (P < 0.05). Colors from green to red represent the miRNA expression abundance from poor to rich.</p

The<i>TGFB1</i> Functional Polymorphism rs1800469 and Susceptibility to Atrial Fibrillation in Two Chinese Han Populations

<div><p>Transforming growth factor-β1 (TGF-β1) is related to the degree of atrial fibrosis and plays critical roles in the induction and perpetuation of atrial fibrillation (AF). To investigate the association of the common promoter polymorphism rs1800469 in the TGF-β1 gene (<i>TGFB1</i>) with the risk of AF in Chinese Han population, we carried out a case-control study of two hospital-based independent populations: Southeast Chinese population (581 patients with AF and 723 controls), and Northeast Chinese population (308 AF patients and 292 controls). Two hundred and seventy-eight cases of AF were lone AF and 334 cases of AF were diagnosed as paroxysmal AF. In both populations, AF patients had larger left atrial diameters than the controls did. The rs1800469 genotypes in the <i>TGFB1</i> gene were determined by polymerase chain reaction-restriction fragment length polymorphism. The genotype and allele frequencies of rs1800469 were not different between AF patients and controls of the Southeast Chinese population, Northeast Chinese population, and total Study Population. After adjustment for age, sex, hypertension and LAD, there was no association between the rs1800469 polymorphism and the risk of AF under the dominant, recessive and additive genetic models. Similar results were obtained from subanalysis of the lone and paroxymal AF subgroups. Our results do not support the role of the <i>TGFB1</i> rs1800469 functional gene variant in the development of AF in the Chinese Han population.</p> </div