Additional file 1 of Saikosaponin D alleviates inflammatory response of osteoarthritis and mediates autophagy via elevating microRNA-199-3p to target transcription Factor-4

Additional file 1: Figure S1. Effects of SSD and miR-199-3p on anabolic activity and catabolic activity of chondrocytes. A-D. Western blot detected Col2a1 and MMP-13. Col2a1 protein decreased and MMP-13 protein increased in OA chondrocytes. SSD treatment inhibited the expression of Col2a1 protein and promoted the expression of MMP-13 protein. Downregulation of miR-199-3p decreased the effect of SSD on the expression of Col2a1 and MMP-13 proteins. After upregulation of miR-199-3p alone, the expression of Col2a1 protein was increased, and the expression of MMP-13 protein was decreased; while, upregulation of TCF4 could reduce the influence of upregulation of miR-199-3p on the expression of Col2a1 and MMP-13 protein. * P < 0.05, ** P < 0.01. N = 3

An approximate cone beam reconstruction algorithm for gantry-tilted CT

Abstract not reproduced here by request of the publisher. The text is available from: http://dx.doi.org/10.1117/12.653121

11-Step Total Synthesis of Araiosamines

A concise route to a small family of exotic marine alkaloids known as the araiosamines has been developed, and their absolute configuration has been assigned. The dense array of functionality, high polarity, and rich stereochemistry coupled with equilibrating topologies present an unusual challenge for chemical synthesis and an opportunity for innovation. Key steps involve the use of a new reagent for guanidine installation, a remarkably selective C–H functionalization, and a surprisingly simple final step that intersects a presumed biosynthetic intermediate. Synthetic araiosamines were shown to exhibit potency against Gram-positive and -negative bacteria despite a contrary report of no activity

11-Step Total Synthesis of Araiosamines

A concise route to a small family of exotic marine alkaloids known as the araiosamines has been developed, and their absolute configuration has been assigned. The dense array of functionality, high polarity, and rich stereochemistry coupled with equilibrating topologies present an unusual challenge for chemical synthesis and an opportunity for innovation. Key steps involve the use of a new reagent for guanidine installation, a remarkably selective C–H functionalization, and a surprisingly simple final step that intersects a presumed biosynthetic intermediate. Synthetic araiosamines were shown to exhibit potency against Gram-positive and -negative bacteria despite a contrary report of no activity

11-Step Total Synthesis of Araiosamines

A concise route to a small family of exotic marine alkaloids known as the araiosamines has been developed, and their absolute configuration has been assigned. The dense array of functionality, high polarity, and rich stereochemistry coupled with equilibrating topologies present an unusual challenge for chemical synthesis and an opportunity for innovation. Key steps involve the use of a new reagent for guanidine installation, a remarkably selective C–H functionalization, and a surprisingly simple final step that intersects a presumed biosynthetic intermediate. Synthetic araiosamines were shown to exhibit potency against Gram-positive and -negative bacteria despite a contrary report of no activity

11-Step Total Synthesis of Araiosamines

A concise route to a small family of exotic marine alkaloids known as the araiosamines has been developed, and their absolute configuration has been assigned. The dense array of functionality, high polarity, and rich stereochemistry coupled with equilibrating topologies present an unusual challenge for chemical synthesis and an opportunity for innovation. Key steps involve the use of a new reagent for guanidine installation, a remarkably selective C–H functionalization, and a surprisingly simple final step that intersects a presumed biosynthetic intermediate. Synthetic araiosamines were shown to exhibit potency against Gram-positive and -negative bacteria despite a contrary report of no activity

Asymmetric Organocatalytic Double-Conjugate Addition of Malononitrile to Dienones: Efficient Synthesis of Optically Active Cyclohexanones

9-Amino-9-deoxyepiquinine efficiently catalyzed the double-conjugate addition of malononitrile to dienones. A number of 1,1,2,6-tetrasubstituted cyclohexanones were prepared in good yields, diastereoselectivities, and excellent enantioselectivities

Earth's Future (Reconceptualized Tree Cooling Efficiency by Constraining Urban Heterogeneity, [Paper # 2023EF004185])

In-situ measurement of air temperature in two adjacent sites in Beijing during the hot summer</p

A systematic evaluation for the potential translation of CD166-related expression as a cancer biomarker

<p><b>Introduction</b>: Many basic studies have provided some evidences for the correlations of CD166 to cancer. However, along with the growing studies on the clinical values of CD166 in cancer areas, some controversial and inconclusive results were obtained.</p> <p><b>Areas covered</b>: An appropriate query and collection of the published articles was conducted through search in PubMed and EMBASE database. A subsequent systematical and quantitative summary of CD166 related expression and cancer was conducted with meta-analysis to clarify its clinical significance for potential translation as cancer biomarkers.</p> <p><b>Expert commentary</b>: The overall results suggested total CD166 correlated to cancer risk, membrane CD166 correlated to nodal metastasis and cytoplasmic CD166 correlated to TNM stage, and disease-free survival. The membrane CD166, cytoplasmic CD166 and soluble CD166 showed great potential to be used as a panel of markers for predicting cancer overall survival. We might conclude that CD166 functions as a risk factor for cancers, and the alterations of its different functional isoforms were observed to correlate with specific or interplayed clinical outcomes.</p

Additional file 1 of Circular RNA hsa_circ_0000277 promotes tumor progression and DDP resistance in esophageal squamous cell carcinoma

Additional file 1: Supplementary Fig. 1. The detection of apoptotic proteins. (A-H) The protein levels of cleaved-PARP and cleaved-caspase3 were detected by western blot for Fig. 3K-L (A-B), Fig. 5M-N (C-D), Fig. 7L-M (E-F) and Fig. 9 (G-H). *P < 0.05. Supplementary Fig. 2. Knockdown of hsa_circ_0000277 reduced proliferation and induced apoptosis in ESCC cells. (A-B) Cell proliferation by EdU assay (A) and apoptosis by flow cytometry (B) were performed after EC9706 and KYSE30 cells were transfected with sh-NC, sh-circ_0000277#1, sh-circ_0000277#2. *P < 0.05