E-cigarettes, is this alternative safe?

Electronic cigarettes are often presented as a safer alternative to classic cigarettes. We present an overview of the latest data on the safety of using e-cigarettes and their impact on the respiratory and cardiovascular systems. The statement that e-cigarettes are much less harmful seems too hasty due to the relatively short presence of e-cigarettes on the market. We need more years of research on large groups of patients to help us answer the question whether e-cigarettes are a better and safer form of smoking

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts. June 4-7, 2019, Szczyrk, Polan

Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients.

microRNA (miRNA) belongs to the non-coding RNAs family responsible for the regulation of gene expression. Renalase is a protein composed of 342 amino acids, secreted by the kidneys and possibly plays an important role in the regulation of sympathetic tone and blood pressure. The aim of the present study was to investigate plasma renalase concentration, and explore the relationship between miRNA-146a-5p expression and plasma renalase levels in hemodialyzed patients.The study population comprised 55 subjects who succumbed to various cardiac events, 27 women and 28 men, aged 65-70 years. The total RNA including miRNA fraction was isolated using QiagenmiRNEasy Serum/Plasma kit according to the manufacturer's protocol. The isolated miRNAs were analyzed using a quantitative polymerase chain reaction (qRT-PCR) technique. The plasma renalase levels were measured using a commercial ELISA kit.In the group of patients with high levels of renalase, higher miRNA-146a expression was found, compared with those with low concentration of renalase. Patients with simultaneous low miRNA-146a expression and high level of renalase were confirmed to deliver a significantly longer survival time compared with other patients.miRNA-146a and plasma renalase levels were estimated as independent prognostic factors of hemodialyzed patients' survival time. Patients with low miRNA-146a expression demonstrated a significantly longer survival time in contrast to the patients with a high expression level of miRNA-146a. Moreover, a significantly longer survival time was found in patients with high renalase activity compared with patients with low activity of the enzyme

Probability of overall survival change depending on miRNA-146a expression level in the studied group.

<p>Low miRNA-146a expression level is a favorable prognostic factor of patients’ survival compared with patients with high expression of this miRNA (OS—69 vs. 56 months; p = 0.430; HR = 1.728).</p

Baseline characteristics in long-term dialysis patients.

<p>Baseline characteristics in long-term dialysis patients.</p

Raw Data Plos One.docx

Raw data used for the preparation of a manuscript entitled "Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients", which is published in the Plos One journal <br

Probability of overall survival change depending on simultaneous analysis of renalase concentration and miRNA-146a expression level in hemodialyzed patients.

<p>Patients with simultaneous high concentration of renalase and low miRNA-146a expression demonstrate significantly higher OS compared with other studied patients (OS—77 vs. 39 months; p = 0.018; HR = 2.01).</p