Additional file 1 of Prediction of circRNA-disease associations based on inductive matrix completion

Additional file 1. Supplementary file to this work (Table S1-S2 and Figures S1-S11)

Multifocal Raman Spectrophotometer for Examining Drug-Induced and Chemical-Induced Cellular Changes in 3D Cell Spheroids

Cell spheroids offer alternative in vitro cell models to monolayer cultured cells because they express complexities similar to those of in vivo tissues, such as cellular responses to drugs and chemicals. Raman spectroscopy emerged as a powerful analytical tool for detecting chemical changes in living cells because it nondestructively provides vibrational information regarding a target. Although multiple iterations are required in drug screening to determine drugs to treat cell spheroids and assess the inter-spheroid heterogeneity, current Raman applications used in spheroids analysis allow the observation of only a few spheroids owing to the low throughput of Raman spectroscopy. In this study, we developed a multifocal Raman spectrophotometer that enables simultaneous analysis of multiple spheroids in separate wells of a regular 96-well plate. By utilizing 96 focal spots excitation and parallel signal collection, our system can improve the throughput by approximately 2 orders of magnitude compared to a conventional single-focus Raman microscope. The Raman spectra of HeLa cell spheroids treated with anticancer drugs and HepG2 cell spheroids treated with free fatty acids were measured simultaneously, and concentration-dependent cellular responses were observed in both studies. Using the multifocal Raman spectrophotometer, we rapidly observed chemical changes in spheroids, and thus, this system can facilitate the application of Raman spectroscopy in analyzing the cellular responses of spheroids

Enhanced Energy Storage Performance Achieved in Multilayered PVDF–PMMA Nanocomposites Incorporated with High-Entropy Oxide Nanofibers

Large depletion of fossil fuels promotes the sustainable development of renewable energy. Polymer-based dielectric nanocomposites are widely utilized owing to their ultrafast charging–discharging rate and high power density in pulse power systems, smart grids, and other electrical devices. In this paper, completely new high-entropy oxide (Eu0.2Bi0.2Y0.2La0.2Cr0.2)2O3 (in short, E) nanofibers are fabricated, and the multilayered nanocomposites are prepared with electrospinning and layer-by-layer hot-pressing processes. A high energy density of 20.11 J cm–3 and an efficiency of 64% are achieved at 598.9 kV mm–1 in the trilayered nanocomposites. First, the enhanced entropy of the lattices leads to local polymorphic distortion, which, to a certain extent, limits the movements of long polymer chains, reduces the dielectric loss, and improves the breakdown strength of the nanocomposites. Moreover, for multilayered nanocomposites, the Schottky barrier forms with the direct contact between the electrodes and ferroelectric polymer. The difference in permittivity of the materials in contact with the electrodes leads to the difference in the barrier height around the interfaces, which results in a larger local electric field and a higher breakdown strength. Besides, the multilayered nanocomposites improve the rough interfaces between inorganics and organics and reduce the probability of dielectric breakdown. This work provides a new filling strategy and structural-layer design for the application of flexible dielectric materials

Label-Free Monitoring of Drug-Induced Cytotoxicity and Its Molecular Fingerprint by Live-Cell Raman and Autofluorescence Imaging

Simultaneous observation of drug distribution at the effector site and subsequent cell response are essential in the drug development process. However, few studies have visualized the drug itself and biomolecular interactions in living cells. Here, we used label-free Raman microscopy to investigate drug-induced cytotoxicity and visualize drug uptake and subcellular localization by its specific molecular fingerprint. A redox-sensitive Raman microscope detected the decrease of reduced cytochrome c (cyt c) after Actinomycin D (ActD) treatment in a time-dependent and dose-dependent format. Immunofluorescence staining of cyt c suggested that the release of cyt c was not the major cause. Combining Raman microscopy with conventional biological methods, we reported that the oxidization of cyt c is an early cytotoxicity marker prior to the release of cyt c. Moreover, as the spectral properties of ActD are sensitive to the surrounding environment, subcellular localization of ActD was visualized sensitively by the weak autofluorescence, and the intercalation of ActD into DNA was detected by shifted Raman peaks, allowing for parallel observation of drug uptake and the mechanism of action. In this research, we achieved simultaneous observation of cytotoxicity and cellular drug uptake by Raman microscopy, which could facilitate a precise understanding of pharmacological effects and predict potential drug toxicity in the future

Supplementary document for Bessel-beam illumination Raman microscopy - 5811531.pdf

The imaging property and the detail of the optical setu

Supplementary document for Bessel-beam illumination Raman microscopy - 5700625.pdf

The imaging property and the detail of the optical setu

Individual Cloud-Based Fingerprint Operation Platform for Latent Fingerprint Identification Using Perovskite Nanocrystals as Eikonogen

Fingerprint formed through lifted papillary ridges is considered the best reference for personal identification. However, the currently available latent fingerprint (LFP) images often suffer from poor resolution, have a low degree of information, and require multifarious steps for identification. Herein, an individual Cloud-based fingerprint operation platform has been designed and fabricated to achieve high-definition LFPs analysis by using CsPbBr3 perovskite nanocrystals (NCs) as eikonogen. Moreover, since CsPbBr3 NCs have a special response to some fingerprint-associated amino acids, the proposed platform can be further used to detect metabolites on LFPs. Consequently, in virtue of Cloud computing and artificial intelligence (AI), this study has demonstrated a champion platform to realize the whole LFP identification analysis. In a double-blind simulative crime game, the enhanced LFP images can be easily obtained and used to lock the suspect accurately within one second on a smartphone, which can help investigators track the criminal clue and handle cases efficiently

Ion–Dipole-Interaction-Induced Encapsulation of Free Residual Solvent for Long-Cycle Solid-State Lithium Metal Batteries

Owing to high ionic conductivity and mechanical strength, poly­(vinylidene fluoride) (PVDF) electrolytes have attracted increasing attention for solid-state lithium batteries, but highly reactive residual solvents severely plague cycling stability. Herein, we report a free-solvent-capturing strategy triggered by reinforced ion–dipole interactions between Li+ and residual solvent molecules. Lithium difluoro­(oxalato)­borate (LiDFOB) salt additive with electron-withdrawing capability serves as a redistributor of the Li+ electropositive state, which offers more binding sites for residual solvents. Benefiting from the modified coordination environment, the kinetically stable anion-derived interphases are preferentially formed, effectively mitigating the interfacial side reactions between the electrodes and electrolytes. As a result, the assembled solid-state battery shows a lifetime of over 2000 cycles with an average Coulombic efficiency of 99.9% and capacity retention of 80%. Our discovery sheds fresh light on the targeted regulation of the reactive residual solvent to extend the cycle life of solid-state batteries

A Bis-Stabilized Interface Strategy for Low‑<i>k</i> Benzocyclobutene-Based Hollow Silica Nanocomposites

A bis-stabilized interface strategy has been proposed to prepare high-performance low-dielectric (low-k) benzocyclobutene (BCB)-based nanocomposites by a rational-designed and facile-synthesized difunctional silane modifier. Hollow silica nanoparticles (HSNs) can be efficiently surface-modified with the difunctional silane to be evenly dispersed and chemically cross-linked with the BCB resin matrix during the post-thermal curing process, finally obtaining a series of HSNs-doped organic–inorganic nanocomposites. The dielectric properties of the resultant nanocomposites can be effectively adjusted by changing the doping ratios and cavity diameters of HSNs. Compared with commercial BCB resin, the dielectric constant of resultant 0.25 wt % HSNs-doped nanocomposites with a cavity diameter of 180 nm can be greatly reduced to 2.12 by 20%, maintaining the low level (10–4 at 1 kHz) of the dielectric loss, excellent thermal stability, and hydrophobicity as well. This study provides a facile and universal strategy to fabricate low-k BCB nanocomposites for future high-density advanced packaging applications