5 research outputs found

    DataSheet1_Double cross-linked graphene oxide hydrogel for promoting healing of diabetic ulcers.pdf

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    This study explores the synthesis and characterization of a novel double cross-linked hydrogel composed of polyvinyl alcohol (PVA), sodium alginate (SA), graphene oxide (GO), and glutathione (GSH), henceforth referred to as PVA/SA/GO/GSH. This innovative hydrogel system incorporates two distinct types of cross-linking networks and is meticulously engineered to exhibit sensitivity to high glucose and/or reactive oxygen species (ROS) environments. A sequential approach was adopted in the hydrogel formation. The initial phase involved the absorption of GSH onto GO, which was subsequently functionalized with boric acid and polyethylene glycol derivatives via a bio-orthogonal click reaction. This stage constituted the formation of the first chemically cross-linked network. Subsequently, freeze-thaw cycles were utilized to induce a secondary cross-linking process involving PVA and SA, thereby forming the second physically cross-linked network. The resultant PVA/SA/GO/GSH hydrogel retained the advantageous hydrogel properties such as superior water retention capacity and elasticity, and additionally exhibited the ability to responsively release GSH under changes in glucose concentration and/or ROS levels. This feature finds particular relevance in the therapeutic management of diabetic ulcers. Preliminary in vitro evaluation affirmed the hydrogel’s biocompatibility and its potential to promote cell migration, inhibit apoptosis, and exhibit antibacterial properties. Further in vivo studies demonstrated that the PVA/SA/GO/GSH hydrogel could facilitate the healing of diabetic ulcer sites by mitigating oxidative stress and regulating glucose levels. Thus, the developed PVA/SA/GO/GSH hydrogel emerges as a promising candidate for diabetic ulcer treatment, owing to its specific bio-responsive traits and therapeutic efficacy.</p

    DataSheet1_GSK-J1-loaded, hyaluronic acid-decorated metal-organic frameworks for the treatment of ovarian cancer.docx

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    Despite intensive research, ovarian cancer has the highest mortality rates among gynecological malignancies, partly because of its rapid acquisition of chemoresistance to platinum therapy. Hence, strategies are needed to effectively treat carboplatin-resistant ovarian cancer. In this study, we designed and prepared hyaluronic acid-decorated metal-organic frameworks for the targeted delivery of GSK-J1, a JMJD3 demethylase inhibitor (HA@MOF@GSK-J1) for the synergistic treatment of carboplatin-resistant ovarian cancer. HA@MOF@GSK-J1 showed outstanding effectiveness in the inhibition of ovarian cancer in vitro. Furthermore, HA@MOF@GSK-J1 demonstrated higher induction of apoptosis, reduced cell motility, and diminished cell spheroids by attenuating HER2 activity through the effectual activation of H3K27 methylation in its promoter area. Finally, our in vivo results confirmed that HA@MOF@GSK-J1 had better treatment efficacy for carboplatin-resistant ovarian tumor xenografts. Our results highlight the potential of HA@MOF@GSK-J1 as an effective strategy to improve the treatment of carboplatin-resistant ovarian cancer.</p

    Multiple effects of glycine on stability and rheology behavior of hydrated Mg(OH)<sub>2</sub> slurry

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    Hydration of MgO is a potential technology for economically preparing Mg(OH)2 slurry. Understanding the rheological behavior evolution of the slurry during MgO hydration, particularly in the presence of hydration agent, is essential for better control of the hydrated Mg(OH)2 slurry’s rheology. In this work, Mg(OH)2 slurry was prepared by hydrating MgO in the presence of glycine. The effect of glycine on the rheological behavior of the hydrated Mg(OH)2 slurry have been investigated by means of viscosity, shear stress, sedimentation measurements, particle size distribution, 1H nuclear magnetic resonance relaxometry and zeta potential. Results showed that shear thinning rheological slurry could be made by hydration at 80 °C for 1 h. The yield stress of the slurry is 27.49 ± 0.81 Pa, and no settling occurs within 7 days. Yet, in the absence of glycine, the structure of the slurry was unstable and easily settled even after 8 h of hydration. Glycine has multiple effects on the slurry’s solid loading, particle morphology, particle size distribution, and zeta potential of the slurry. In addition, the neutral zwitterionic form of glycine may serve as an inter-particle lubricant, reducing viscosity.</p

    Effect of particle gradation on the properties of Mg(OH)<sub>2</sub> slurry: viscosity, stability, and rheology

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    In this article, multipeak Mg(OH)2 slurries, that is, unimodal, bimodal, and trimodal, were prepared by blending Mg(OH)2 of distinct particle sizes (d50 of 1, 3, 7, 10, and 20 μm). The effects of particle gradation on the properties of Mg(OH)2 slurry, such as viscosity, stability, and rheological behavior, were investigated. Also, the packing efficiency was analyzed by the compartment packing model. The results revealed that viscosity and stability decrease with particle size or larger particle mixing in unimodal and bimodal schemes. However, trimodal slurry viscosity did not significantly change with particle size ratio. The packing efficiency calculated by the compartment packing model has the opposite trend of viscosity, but this trend is not so strictly consistent in the three-peak gradation scheme. Among the unimodal, bimodal, and trimodal slurries with better viscosity and stability (10, 3 + 10 (3:7), and 1 + 7 + 10 μm (3:1:6)), the trimodal slurry had the lowest viscosity and the highest stability. Its highest yield stress (4.66 ± 0.23 Pa) and flow stress (7.67 ± 0.38 Pa) indicated its structural stability, and it showed good structural recovery capability, reestablishing about 87% in 60 seconds. This might be explained by the fine particles forming a bridge between the coarse particles, resulting in a stable and networked structure.</p

    Data_Sheet_1_The composition of phenolic compounds in Chinese olive (Canarium album L.) cultivars and their contribution to the anti-inflammatory properties of the cultivars.docx

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    ObjectiveThis study aimed to explore the phenolic compounds (PCs) present in three Chinese olive (Canarium album L.) cultivars and the contribution of these PCs to the anti-inflammatory activities of the cultivars.MethodsUltra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap/mass spectrometry (UPLC-Q-Exactive/MS) was used to identify and quantify the PCs present in three Chinese olive cultivars, “Na zhong,” “Tan xiang,” and “Xiang zhong”. 2,2-diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azinobis (3-ethylbenzothiazoline 6-sulfonate) (ABTS); and oxygen radical absorption capacity (ORAC) assays were used to assess the antioxidant activities of the PCs. Furthermore, we analyzed the anti-inflammatory action of these PCs using lipopolysaccharide (LPS)-induced RAW264.7 cells.ResultsA total of 44 PCs were identified in the three cultivars. Of these, 17 PCs were previously unidentified in Chinese olive. Among the cultivars, the free phenolics (FPs) of “Tan xiang” showed the strongest antioxidant activity. All cultivars have shown significant inhibition of TNF-α and IL-6 production. Clustering correlation analysis showed galloyl-bis-HHDP-glucose and paeonol have significant anti-inflammatory ability in FPs. Quininic, galloylquinic acid, 4-hydroxycinnamic acid and gallic acid hexoside have shown significant inhibition of IL-6 production in BPs. Furthermore, gallic acid, catechin, syringic acid, and nobiletin exhibit negative correlation in FPs and positive correlation in BPs of cytokine production, while corilagin and methyl ellagic acid pentoside exhibited opposite correlation.ConclusionIn summary, this study contributed to the literature on PCs in Chinese olives and the potential health benefits of FPs and BPs.</p