Preparation and Characterization of <i>N</i>-Alkyl-<i>Se</i>-alkylselenocarbamates

Several N-alkyl-Se-alkylselenocarbamates were prepared. The structure was confirmed by X-ray diffraction. N-Alkyl-Se-alkylselenocarbamates showed interesting NMR spectral features in 13C and 77Se NMR. They could be explained by the equilibrium between the major s-trans and the minor s-cis forms. This assumption was confirmed by the theoretical calculations

Classification of Environmental Estrogens by Physicochemical Properties Using Principal Component Analysis and Hierarchical Cluster Analysis

A structurally diverse assortment of 60 environmental estrogens was divided into two main clusters (“A”, “B”) and a pair of subclusters (“C1”, “C2”) by applying principal component analysis to selected 1D and 2D molecular descriptors and subjecting the PCs to hierarchical cluster analysis. Although clustering was predicated solely on physicochemical properties, the dependence on particular physicochemical parameters of xenoestrogen binding affinities (pKi) to murine uterine cytosolic estrogen receptor (ER) proved greater for compounds within (sub)clusters than for compounds between (sub)clusters. Quantitative structure-binding affinity relationships derived using molecular descriptors and PCs suggested differences in the driving forces for xenoestrogen-ER binding for different (sub)clusters. The modeling power for xenoestrogen-ER binding affinities of a combination of TLSER and WHIM 3D indices was much greater than that of combinations of 1D and 2D molecular descriptors or the PCs derived therefrom. The clusterings obtained using PCs also proved applicable to the 3D-QSARs

Supplementary Figure 1 from The Prognostic Significance of Vasohibin-1 Expression in Patients with Upper Urinary Tract Urothelial Carcinoma

PDF file - 169K, Supplementary Figure 1A. Kaplan-Meier recurrence-free survival of the 171 patients after surgery for UTUC stratified according to the cut-off level of MVD 70/mm2: High level of MVD was not an independent predictor of tumor recurrence (P = 0.216). Supplementary Figure 1B. Kaplan-Meier cancer-specific survival of the 171 patients after surgery for UTUC stratified according to the cut-off level of MVD 70/mm2: High level of MVD was not an independent predictor of cancer-specific survival (P = 0.473). Supplementary Figure 1C. Kaplan-Meier recurrence-free survival of the 171 patients after surgery for UTUC stratified according to the cut-off level of MVD 80/mm2: High level of MVD was not an independent predictor of tumor recurrence (P = 0.092). Supplementary Figure 1D. Kaplan-Meier cancer-specific survival of the 171 patients after surgery for UTUC stratified according to the cut-off level of MVD 80/mm2: High level of MVD was not an independent predictor of cancer-specific survival (P = 0.345). Supplementary Figure 1E. Kaplan-Meier recurrence-free survival of the 171 patients after surgery for UTUC stratified according to the cut-off level of VASH1 density 40/mm2: High level of VASH1 density was an independent predictor of tumor recurrence (P = 0.017). Supplementary Figure 1F. Kaplan-Meier cancer-specific survival of the 171 patients after surgery for UTUC stratified according to the cut-off level of VASH1 density 40/mm2: High level of VASH1 density was an independent predictor of cancer-specific survival (P = 0.042). Supplementary Figure 1G. Kaplan-Meier recurrence-free survival of the 171 patients after surgery for UTUC stratified according to the cut-off level of VASH1 density 50/mm2: High level of VASH1 density was an independent predictor of tumor recurrence (P = 0.005). Supplementary Figure 1H. Kaplan-Meier cancer-specific survival of the 171 patients after surgery for UTUC stratified according to the cut-off level of VASH1 density 50/mm2. High level of VASH1 density was an independent predictor of cancer-specific survival (P = 0.027).</p

Supplementary Table 2 from The Prognostic Significance of Vasohibin-1 Expression in Patients with Upper Urinary Tract Urothelial Carcinoma

PDF file - 33K, Correlation of clinicopathological parameters and MVD or Vasohibin-1 expression in the 175 study patients: Patients with high grade tumors and �pT2 had significantly higher levels of VASH1 density</p

Supplementary Table 3 from The Prognostic Significance of Vasohibin-1 Expression in Patients with Upper Urinary Tract Urothelial Carcinoma

PDF file - 31K, Univariate and multivariate analysis for recurrence-free survival and cancer-specific survival in 171 UTUC patients: We used the cutoff value of MVD � 80/mm2 and VASH1 density � 50/mm2 according to the mean values. The levels of VASH1 density were independent predictors for a decrease in recurrence-free (HR = 2.08) and cancer-specific survival (HR = 2.06)</p

Supplementary Figure 2 from The Prognostic Significance of Vasohibin-1 Expression in Patients with Upper Urinary Tract Urothelial Carcinoma

PDF file - 2.9MB, Supplementary Figure 2A. Immunostaining for CD34 in the case of pTa, low grade, and LVI negative UTUC with low VASH1 density: CD34 staining was observed in endothelial cells of microvascular vessels in the tumor lesion. Supplementary Figure 2B. Immunostaining for VASH1: the same case with 2A. VASH1 staining of vascular endothelial cells was negative or negligible. We observed low VASH1 density. Supplementary Figure 2C. Negative control slide: the same case with 2A-2B. The appropriate negative control slide was prepared. Supplementary Figure 2D. Immunostaining for CD34 in the case of pTa, low grade, and LVI negative UTUC with high VASH1 density: CD34 staining was observed in endothelial cells of microvascular vessels in the tumor lesion. Supplementary Figure 2E. Immunostaining for VASH1: the same case with 2D. VASH1 staining of vascular endothelial cells was strong. We observed high VASH1 density. Supplementary Figure 2F. Negative control slide: the same case with 2D-2E. The appropriate negative control slide was prepared. Supplementary Figure 2G. Immunostaining for CD34 in the case of pT3b, high grade, and LVI positive UTUC with low VASH1 density: CD34 staining was observed in endothelial cells of microvascular vessels in the tumor lesion. Supplementary Figure 2H. Immunostaining for VASH1: the same case with 2G. VASH1 staining of vascular endothelial cells was negative or negligible. We observed low VASH1 density. Supplementary Figure 2I. Negative control slide: the same case with 2G-2H. The appropriate negative control slide was prepared. Supplementary Figure 2J. Immunostaining for CD34 in the case of pT3b, high grade, and LVI positive UTUC with high VASH1 density: CD34 staining was observed in endothelial cells of microvascular vessels in the tumor lesion. Supplementary Figure 2K. Immunostaining for VASH1: the same case with 2J. VASH1 staining of vascular endothelial cells was strong. We observed high VASH1 density. Supplementary Figure 2L. Negative control slide: the same case with 2J-2K.</p

Supplementary Table 1 from The Prognostic Significance of Vasohibin-1 Expression in Patients with Upper Urinary Tract Urothelial Carcinoma

PDF file - 13K, Univariate and multivariate analysis for recurrence-free survival and cancer-specific survival of 175 UTUC patients: Multivariate analysis showed that high tumor grade, presence of LVI, positive pN and high VASH1 density were independent predictors of recurrence and cancer-specific survival</p

Supplementary Figure Legends 1-2, Table Legends 1-3 from The Prognostic Significance of Vasohibin-1 Expression in Patients with Upper Urinary Tract Urothelial Carcinoma

PDF file - 38K</p