第774回 千葉医学会例会・第二内科例会 54.

InterProScan results. InterProScan [34] queries sequences against 16 member databases enabling protein classification by family as well as by conserved domains; the number of annotations and the percent of sequences receiving annotations varied across the different databases. (XLSX 32 kb

A novel molecular toolkit for rapid detection of the pathogen and primary vector of thousand cankers disease

<div><p>Thousand Cankers Disease (TCD) of <i>Juglans</i> and <i>Pterocarya</i> (Juglandaceae) involves a fungal pathogen, <i>Geosmithia morbida</i>, and a primary insect vector, <i>Pityophthorus juglandis</i>. TCD was described originally from dying <i>Juglans nigra</i> trees in the western United States (USA), but it was reported subsequently from the eastern USA and northern Italy. The disease is often difficult to diagnose due to the absence of symptoms or signs on the bark surface of the host. Furthermore, disease symptoms can be confused with those caused by other biotic and abiotic agents. Thus, there is a critical need for a method for rapid detection of the pathogen and vector of TCD. Using species-specific microsatellite DNA markers, we developed a molecular protocol for the detection of <i>G</i>. <i>morbida</i> and <i>P</i>. <i>juglandis</i>. To demonstrate the utility of the method for delineating TCD quarantine zones, we tested whether geographical occurrence of symptoms and signs of TCD was correlated with molecular evidence for the presence of the cryptic TCD organisms. A total of 1600 drill cores were taken from branch sections collected from three regions (<i>n</i> = 40 trees for each location): California-<i>J</i>. <i>hindsii</i> (heavy disease incidence); Tennessee-<i>J</i>. <i>nigra</i> (mild disease incidence); and outside the known TCD zone (Missouri-<i>J</i>. <i>nigra</i>, no record of the disease). California samples had the highest incidence of the TCD organisms (85%, 34/40). Tennessee had intermediate incidence (42.5%, 17/40), whereas neither organism was detected in samples from Missouri. The low cost molecular protocol developed here has a high degree of sensitivity and specificity, and it significantly reduces sample-processing time, making the protocol a powerful tool for rapid detection of TCD.</p></div

Molecular detection of Thousand Cankers Disease

Molecular detection of Thousand Cankers Diseas

Molecular detection of thousand cankers disease (percentage of positive drill samples) from both feature-directed and lesion-directed samples from California, Missouri (control), and Tennessee.

<p>Molecular detection of thousand cankers disease (percentage of positive drill samples) from both feature-directed and lesion-directed samples from California, Missouri (control), and Tennessee.</p

Molecular detection outcomes among 40 samples each from California (<i>Juglans hindsii</i>), Missouri (control, <i>Juglans nigra</i>) and Tennessee (<i>J</i>. <i>nigra</i>) for both drilling methods.

<p>Molecular detection outcomes among 40 samples each from California (<i>Juglans hindsii</i>), Missouri (control, <i>Juglans nigra</i>) and Tennessee (<i>J</i>. <i>nigra</i>) for both drilling methods.</p

Comparisons of molecular detection of <i>Geosmithia morbida</i> (<i>Gm</i>) and <i>Pityophthorus juglandis</i> (<i>Pj</i>) in any of the drilled samples (per tree) confirmed that a sample (tree) is positive for either organism^A.

<p>Comparisons of molecular detection of <i>Geosmithia morbida</i> (<i>Gm</i>) and <i>Pityophthorus juglandis</i> (<i>Pj</i>) in any of the drilled samples (per tree) confirmed that a sample (tree) is positive for either organism<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185087#t002fn002" target="_blank">^A</a>.</p

Molecular detection of thousand cankers disease organisms (<i>Geosmithia morbida</i> and/or <i>Pityophthorus juglandis</i>) among positive trees for all samples and both drilling methods.

<p>Molecular detection of thousand cankers disease organisms (<i>Geosmithia morbida</i> and/or <i>Pityophthorus juglandis</i>) among positive trees for all samples and both drilling methods.</p

El Diario de Pontevedra : periódico liberal: Ano XLIII Número 12518 - 1926 outubro 7

All informative microsatellite markers and associated data. In total 116 informative markers were developed for Q. rubra. These include 37 gSSRs, 38 EST-SSRs from Q. rubra, 1 C. mollissima EST-SSR and 40 Q. robur EST-SSRs. The table shows the marker name, the corresponding sequence accession numbers from NCBI, forward and reverse primers used for amplification and base pair sizes in Q. rubra mapping parents. (XLSX 78 kb

Number of PALs (potentially amplifiable loci) for each of ten hardwood tree species.

<p>Hundreds to thousands of PALs were identified for each species sequenced. For all species the most commonly identified repeat motif was 2 bases, followed by 3 base motifs. Reptitive motifs of 4 bases were found the least often.</p

Identified repetitive elements and genes in genomic reads.

<p>The percent of reconstructed fragments with sequence similarity to known plant repetitive elements and gene sequences vary across species. The majority of identified repetitive elements originate from the retrotransposon classes of Gypsy and Copia.</p