MIAPPE checklist

MIAPPE checklist dat

Image flattening program

Image flattening progra

OCT images of Apple and Persimmon leaves

OCT images of Apple and Persimmon leaves used in this stud

A scan profiling program

A scan profiling progra

Biodegradable Photonic Melanoidin for Theranostic Applications

Light-absorbing nanoparticles for localized heat generation in tissues have various biomedical applications in diagnostic imaging, surgery, and therapies. Although numerous plasmonic and carbon-based nanoparticles with strong optical absorption have been developed, their clearance, potential cytotoxicity, and long-term safety issues remain unresolved. Here, we show that “generally regarded as safe (GRAS)” melanoidins prepared from glucose and amino acid offer a high light-to-heat conversion efficiency, biocompatibility, biodegradability, nonmutagenicity, and efficient renal clearance, as well as a low cost for synthesis. We exhibit a wide range of biomedical photonic applications of melanoidins, including <i>in vivo</i> photoacoustic mapping of sentinel lymph nodes, photoacoustic tracking of gastrointestinal tracts, photothermal cancer therapy, and photothermal lipolysis. The biodegradation rate and renal clearance of melanoidins are controllable by design. Our results confirm the feasibility of biodegradable melanoidins for various photonic applications to theranostic nanomedicines

Porphyrin Shell Microbubbles with Intrinsic Ultrasound and Photoacoustic Properties

Porphyrin–phospholipid conjugates were used to create photonic microbubbles (MBs) having a porphyrin shell (“porshe”), and their acoustic and photoacoustic properties were investigated. The inclusion of porphyrin–lipid in the MB shell increased the yield, improved the serum stability, and generated a narrow volumetric size distribution with a peak size of 2.7 ± 0.2 μm. Using an acoustic model, we calculated the porshe stiffness to be 3–5 times greater than that of commercial lipid MBs. Porshe MBs were found to be intrinsically suitable for both ultrasound and photoacoustic imaging with a resonance frequency of 9–10 MHz. The distinctive properties of porshe MBs make them potentially advantageous for a broad range of biomedical imaging and therapeutic applications

Au–Cu_2–<i>x</i>Se Heterodimer Nanoparticles with Broad Localized Surface Plasmon Resonance as Contrast Agents for Deep Tissue Imaging

We report a new type of heterogeneous nanoparticles (NPs) composed of a heavily doped semiconductor domain (Cu_2–<i>x</i>Se) and a metal domain (Au), which exhibit a broad localized surface plasmon resonance (LSPR) across visible and near-infrared (NIR) wavelengths, arising from interactions between the two nanocrystal domains. We demonstrate both in vivo photoacoustic imaging and in vitro dark field imaging, using the broad LSPR in Cu_2–<i>x</i>Se–Au hybrid NPs to achieve contrast at different wavelengths. The high photoacoustic imaging depth achieved, up to 17 mm, shows that these novel contrast agents could be clinically relevant. More broadly, this work demonstrates a new strategy for tuning LSPR absorbance by engineering the density of free charge carriers in two interacting domains

Figure S7 from A Peptide Probe Enables Photoacoustic-Guided Imaging and Drug Delivery to Lung Tumors in <i>K-ras^LA2</i> Mutant Mice

Fig. S7. Analysis of hematologic parameters and liver and kidney function after treatment with untargeted and the CRQTKN-targeted liposomes in K-rasLA2 mutant mice.</p

Figure S8 from A Peptide Probe Enables Photoacoustic-Guided Imaging and Drug Delivery to Lung Tumors in <i>K-ras^LA2</i> Mutant Mice

Fig. S8. Experimental scheme for the identification of the receptor of the CRQTKN peptide.</p

Figure S4 from A Peptide Probe Enables Photoacoustic-Guided Imaging and Drug Delivery to Lung Tumors in <i>K-ras^LA2</i> Mutant Mice

Fig. S4. PA sensitivity and spectrum of FPI774 NIR fluorescence dye-labeled CRQTKN peptide in vitro.</p