Levels of Th1/Th2 cytokine mRNA RT-PCR production of mice spleens.

<p>The PCR amplified fragments were analyzed on agarose gel. The optical density of each band was measured with a computer-assisted image analysis system. Relative expressions of Th1/Th2 cytokine mRNA were analyzed by the ratio of the band density of its amplified fragment over that of β-actin. A: OD values of RT-PCR products from Th1/Th2 cytokine mRNA and β-actin. B: The ratios of Th1/Th2 cytokine mRNA expression. The data in Fig. 4B does not necessarily reflect the exact ratios of each cytokine mRNA to b-actin, because PCR amplification is not exponential at high cycle numbers. *P<0.05 versus control group, ^# P<0.01 versus control group.</p

Th1 and Th2 cytokines in sera of mice.

<p>The levels of IFN-γ IL-2, IL-4, and IL-5 were determined in individual sera (n = 10 mice/group) by commercially available ELISA kits. The experiment was performed twice and each value shown represents the mean and SE of 20 value. * P<0.05 versus control group. ^# P<0.01 versus control group.</p

Levels of anti-HBsAg antibody in sera of mice.

<p>Anti-HBsAg antibody was individually determined by a commercial enzyme-linked immunoassay. Two separate experiments (n = 10 mice/group) were performed and the data from two independent experiments were pooled and so each value shown represents the means and SE of 20 value. * P<0.05 versus control group. ^# P<0.01 versus control group.</p

Concentrations of Th1and Th2 cytokines in spleen cell culture supernatants of mice.

<p>Spleen cells derived from the different groups at different times were tested under the same conditions. The experiment was performed twice (n = 10 mice/group) and each value shown represents the mean and SE of 20 value. P<0.05 versus control group. ^# P<0.01 versus control group.</p