Supplemental figure 1 from Clinical and Molecular Characteristics of <i>NF1</i>-Mutant Lung Cancer

Gene mutations evaluated in 275 gene panel referenced in the manuscript.</p

Supplemental Tables 1-3, Supplementary Figure Legends from Clinical and Molecular Characteristics of <i>NF1</i>-Mutant Lung Cancer

Supplemental Table 1: Identified NF1 variants; Supplemental Table 2: Tumors with multiple NF1 mutations include splice site, missense, nonsense, and frameshift mutations; Supplemental Table 3: KRAS mutations identified by next-generation sequencing.</p

Fig 4 -

Linear correlation between input DNA concentration and experimentally calculated concentration for EGFR L858R reaction (A) and EGFR exon 19 deletion reaction (B).</p

Fig 5 -

Experimental versus input variant allele fraction for specimens above limit of detection for EGFR L858R (A) and EGFR exon 19 deletion (B). Mean percent error decreases with increasing variant allele fraction for EGFR L858R (C) and EGFR exon 19 deletion (D). Coefficient of variation with respect to variant allele fraction for EGFR L858R (E) and EGFR exon 19 deletion (F).</p

Histogram of turnaround times for droplet digital PCR compared to panel next generation sequencing.

Histogram of turnaround times for droplet digital PCR compared to panel next generation sequencing.</p

S1 Dataset -

(DOCX)</p

Fig 3 -

Relationship between variant allele fraction (VAF), DNA loading concentration, and positive mutation events for EGFR L858R (A) and EGFR exon 19 deletion (B).</p

Number of positive mutant events for <i>EGFR</i> exon 19 deletion reactions.

Number of positive mutant events for EGFR exon 19 deletion reactions.</p

Droplet digital PCR probe design to detect <i>EGFR</i> L858R substitution (A) and <i>EGFR</i> exon 19 deletion (B).

Droplet digital PCR probe design to detect EGFR L858R substitution (A) and EGFR exon 19 deletion (B).</p

Number of positive mutant events for <i>EGFR</i> L858R reactions.

Number of positive mutant events for EGFR L858R reactions.</p