Additional file 2 of Preparation of triangular silver nanoparticles and their biological effects in the treatment of ovarian cancer

Additional file 2: Supplementary figure 2. SEM image of nanomaterial synthesized without citrate

Additional file 1 of Preparation of triangular silver nanoparticles and their biological effects in the treatment of ovarian cancer

Additional file 1: Supplementary figure 1. SEM image of nanomaterial synthesized without PVP

Additional file 3 of Preparation of triangular silver nanoparticles and their biological effects in the treatment of ovarian cancer

Additional file 3: Supplementary figure 3. DLS results of the five types of synthesized tAgNPs were analyzed by Origin software

Serum Amyloid A and Clusterin as Potential Predictive Biomarkers for Severe Hand, Foot and Mouth Disease by 2D-DIGE Proteomics Analysis

<div><p>Hand, foot, and mouth disease (HFMD) affects more than one million children, is responsible for several hundred child deaths every year in China and is the cause of widespread concerns in society. Only a small fraction of HFMD cases will develop further into severe HFMD with neurologic complications. A timely and accurate diagnosis of severe HFMD is essential for assessing the risk of progression and planning the appropriate treatment. Human serum can reflect the physiological or pathological states, which is expected to be an excellent source of disease-specific biomarkers. In the present study, a comparative serological proteome analysis between severe HFMD patients and healthy controls was performed via a two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) strategy. Fifteen proteins were identified as differentially expressed in the sera of the severe HFMD patients compared with the controls. The identified proteins were classified into different groups according to their molecular functions, biological processes, protein classes and physiological pathways by bioinformatics analysis. The up-regulations of two identified proteins, serum amyloid A (SAA) and clusterin (CLU), were confirmed in the sera of the HFMD patients by ELISA assay. This study not only increases our background knowledge about and scientific insight into the mechanisms of HFMD, but also reveals novel potential biomarkers for the clinical diagnosis of severe HFMD.</p></div

Functional classifications of the identified proteins according to their (A) molecular functions, (B) biological processes and (C) protein class by PANTHER.

<p>Functional classifications of the identified proteins according to their (A) molecular functions, (B) biological processes and (C) protein class by PANTHER.</p

2D-DIGE analysis of the protein spots were differentially expressed between the severe HFMD groups and the healthy controls.

<p>(A) The proteins extracted from the HFMD and control samples were labelled with Cy3 and Cy5, respectively. An internal standard protein sample (a mixture of severe HFMD and control samples) was labeled with the Cy2 dye. The green spots represent up-regulated proteins, while the red spots represent down-regulated proteins in the severe HFMD samples compared with the control samples. The yellow arrow represents the identified spots that showed significantly different expression between the severe HFMD and control samples. The number in the figure corresponds to the master number presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108816#pone-0108816-t002" target="_blank">Tables 2</a>. (B) Differentially expressed proteins identified in 2-DE gels.</p

ELISA assay results for the SAA (A) and CLU (B) levels in 20 serum samples from the healthy controls and the severe HFMD groups.

<p>The protein levels were expressed as the mean ± the SD. **<i>p</i><0.01 compared to the healthy controls (independent Student’s <i>t</i>-test).</p

Differentially expressed proteins identified by mass spectrometry (MS) after 2-D DIGE analysis.

<p>Differentially expressed proteins identified by mass spectrometry (MS) after 2-D DIGE analysis.</p

The clinical characteristics of the severe HFMD and normal groups.

<p>The clinical characteristics of the severe HFMD and normal groups.</p