The ChIP-seq-defined networks of Bcl-3 gene binding support its required role in skeletal muscle atrophy

NF-kappaB transcriptional activation is required for skeletal muscle disuse atrophy. We are continuing to study how the activation of NF-kB regulates the genes that encode the protein products that cause atrophy. Using ChIP-sequencing we found that Bcl-3, an NF-kB transcriptional activator required for atrophy, binds to the promoters of a number of genes whose collective function describes two major aspects of muscle wasting. By means of bioinformatics analysis of ChIP-sequencing data we found Bcl-3 to be directing transcription networks of proteolysis and energy metabolism. The proteolytic arm of the Bcl-3 networks includes many E3 ligases associated with proteasomal protein degradation, including that of the N-end rule pathway. The metabolic arm appears to be involved in organizing the change from oxidative phosphorylation to glycolysis in atrophying muscle. For one gene, MuRF1, ChIP-sequencing data identified the location of Bcl-3 and p50 binding in the promoter region which directed the creation of deletant and base-substitution mutations of MuRF1 promoter constructs to determine the effect on gene transcription. The results provide the first direct confirmation that the NF-kB binding site is involved in the muscle unloading regulation of MuRF1. Finally, we have combined the ChIP-sequencing results with gene expression microarray data from unloaded muscle to map several direct targets of Bcl-3 that are transcription factors whose own targets describe a set of indirect targets for NF-kB in atrophy. ChIP-sequencing provides the first molecular explanation for the finding that Bcl3 knockout mice are resistant to disuse muscle atrophy. Mapping the transcriptional regulation of muscle atrophy requires an unbiased analysis of the whole genome, which we show is now possible with ChIP-sequencing.R01 AR041705 - NIAMS NIH HHS; R01 AR060217 - NIAMS NIH HHS; AR041705 - NIAMS NIH HHS; AR060217 - NIAMS NIH HH

Galactic distribution of interstellar Al-26

A narrow cosmic gamma ray line at 1809 keV was discovered which was interpreted as resulting from the decay of approximately 3 M sub theta of Al-26 residing in the galactic disk. While its intrinsic width was unresolved by the HEAO 3 spectrometer, a (1 sigma) limit of 3 keV FWHM was obtained; this corresponds to bulk motions of v 250 km/s, which is consistent with material at rest in the ISM. Sites which have been suggested include type II supernovae and massive stars which are members of the extreme population I, as well as novae and red giants which are associated with an older disk population. The HEAO 3 data was used to distinguish between these two stellar populations

HEAO 3 upper limits to the expected 1634 KeV line from SS 483

A model based on 24 Mg(1369) was developed as the source of the lines in which refractory grains in the jets, containing Mg and 0, are bombarded, by ambient protons in the local ISM. The narrowness of the features results because the recoil Mg nucleus is stopped in the grain before the 1369 keV excited state decays. A consequence of the 24 Mg interpretation is the expected appearance of other emission lines, due to 20 Ne and 20 Na, which are produced by proton bombardment of 24 Mg at the 33 MeV/nucleon energy corresponding to the velocity of the jets. These lines appear at rest energies of 1634 keV and 1636 keV, respectively, and should have essentially the same total flux as that emited at 1369 keV. The HEAO 3 data are examined to search for the 1634 keV (rest) emission. The observation and analysis, the results, and the implications for the understanding of SS 433 are discussed

Anomalous Light Scattering by Topological PT{\mathcal{PT}}-symmetric Particle Arrays

Robust topological edge modes may evolve into complex-frequency modes when a physical system becomes non-Hermitian. We show that, while having negligible forward optical extinction cross section, a conjugate pair of such complex topological edge modes in a non-Hermitian $\mathcal{PT}$-symmetric system can give rise to an anomalous sideway scattering when they are simultaneously excited by a plane wave. We propose a realization of such scattering state in a linear array of subwavelength resonators coated with gain media. The prediction is based on an analytical two-band model and verified by rigorous numerical simulation using multiple-multipole scattering theory. The result suggests an extreme situation where leakage of classical information is unnoticeable to the transmitter and the receiver when such a $\mathcal{PT}$-symmetric unit is inserted into the communication channel.Comment: 16 pages, 8 figure

Formation of Non-reciprocal Bands in Magnetized Diatomic Plasmonic Chains

We show that non-reciprocal bands can be formed in a magnetized periodic chain of spherical plasmonic particles with two particles per unit cell. Simplified form of symmetry operators in dipole approximations are used to demonstrate explicitly the relation between spectral non-reciprocity and broken spatial-temporal symmetries. Due to hybridization among plasmon modes and free photon modes, strong spectral non-reciprocity appears in region slightly below the lightline, where highly directed guiding of energy can be supported. The results may provide a clear guidance on the design of one-way waveguides

Gamma-ray burst variability above 4 MeV

The relationship between the hard X-ray and gamma ray emissions during four bursts using the anti-coincidence shields of the High Energy Astronomy Observatory 3 (HEAO 3) Gamma Ray Spectrometer is explored. Recent observations of gamma ray bursts by the Solar Maximum Mission Gamma Ray Spectrometer (GRS) have shown that high energy emission above 1 MeV is a common and energetically important feature (Matz et al. 1985). Time histories of four gamma ray bursts in 3 energy bands ( keV, around 511 keV, and 4 MeV) with 10.24 a resolution show that the 4 MeV flux is only weakly coupled to the spectrum below approximately 600 keV

Reexamining the "finite-size" effects in isobaric yield ratios using a statistical abrasion-ablation model

The "finite-size" effects in the isobaric yield ratio (IYR), which are shown in the standard grand-canonical and canonical statistical ensembles (SGC/CSE) method, is claimed to prevent obtaining the actual values of physical parameters. The conclusion of SGC/CSE maybe questionable for neutron-rich nucleus induced reaction. To investigate whether the IYR has "finite-size" effects, the IYR for the mirror nuclei [IYR(m)] are reexamined using a modified statistical abrasion-ablation (SAA) model. It is found when the projectile is not so neutron-rich, the IYR(m) depends on the isospin of projectile, but the size dependence can not be excluded. In reactions induced by the very neutron-rich projectiles, contrary results to those of the SGC/CSE models are obtained, i.e., the dependence of the IYR(m) on the size and the isospin of the projectile is weakened and disappears both in the SAA and the experimental results.Comment: 5 pages and 4 figure

Optimization of aircraft seat cushion fire blocking layers

This report describes work completed by the National Aeronautics and Space Administration - for the Federal Aviation Administration Technical Center. The purpose of this work was to examine the potential of fire blocking mechanisms for aircraft seat cushions in order to provide an optimized seat configuration with adequate fire protection and minimum weight. Aluminized thermally stable fabrics were found to provide adequate fire protection when used in conjunction with urethane foams, while maintaining minimum weight and cost penalty

Fate of the Peak Effect in a Type-II Superconductor: Multicriticality in the Bragg-Glass Transition

We have used small-angle-neutron-scattering (SANS) and ac magnetic susceptibility to investigate the global magnetic field H vs temperature T phase diagram of a single crystal Nb in which a first-order transition of Bragg-glass melting (disordering), a peak effect, and surface superconductivity are all observable. It was found that the disappearance of the peak effect is directly related to a multicritical behavior in the Bragg-glass transition. Four characteristic phase boundary lines have been identified on the H-T plane: a first-order line at high fields, a mean-field-like continuous transition line at low fields, and two continuous transition line associated with the onset of surface and bulk superconductivity. All four lines are found to meet at a multicritical point.Comment: 4 figure

High-resolution spectrum of Cygnus X-1

A high resolution spectrum of Cygnus X-1 in the 45 to 600 keV range is presented. The measurement was made by the HEAO 3 gamma ray spectrometer during 82 days in the fall of 1979 and spring of 1980, when the source was in its normal low state. Results of a search for narrow emission lines from the source are reported. The spectrum shows no significant narrow features. The 3 delta upper limit to a narrow 511 keV annihilation line is 3 x 0.0001 photons/sq cm/s. There is also no evidence in HEAO 3 broadband data above 500 keV for the broad annihilation feature observed by HEAO 1