Individual Professional Practice in the Company

Import 23/08/2017Cílem této bakalářské práce je popsat absolvování odborné praxe ve firmě HS Interactive s.r.o. Praxe byla zaměřena na vývoj mobilní aplikace pro operační systém Android. Aplikace je mobilním klientem pro sociální síť MatchToMe. V úvodu popisuji důvody, které vedly k výběru odborné praxe. Dále se věnuji úkolům, které mi byly zadány s jejich implementací a postupem řešení problémů, které se objevily při vývoji. Závěr práce je věnován zhodnocení získaných zkušeností a dosažených výsledků.Purpose of this bachelor thesis is to describe a professional practice in company HS Interactive s.r.o. Practice was focused on the development of mobile application for the operating system Android. The application is a mobile client for social network MatchToMe. In the introduction I describe reasons that led to the selection of professional practice. Then I describe tasks that I have been awarded with their implementations and process of solution issues that have emerged during development. The conclusion of thesis is dedicated to the evaluation of the experience gained and the results achieved.440 - Katedra telekomunikační technikyvýborn

The boundary curve of the semilunar valve.

<p>The boundary curve of the semilunar valve.</p

Posterior view of the human pulmonary valve (cf. [3]).

<p>Posterior view of the human pulmonary valve (cf. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0199390#pone.0199390.ref003" target="_blank">3</a>]).</p

Visualization of the right half of the valve cusp in three-dimensional space.

<p>(a) is the image of the front view of a single cusp and (b) is the side view.</p

The model for the geometrical shape of a cusp in the pulmonary valve.

<p>The leaflets is considered to be one-third of the cylindrical shell.</p

A schematic plot of the human pulmonary valve.

<p>(a) is the pulmonary valve during diastole (cf. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0199390#pone.0199390.ref015" target="_blank">15</a>]) and (b) is the pulmonary valve during systole (cf. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0199390#pone.0199390.ref018" target="_blank">18</a>]) (c) is the expanded semilunar valve. A: anterior leaflet, L: left leaflet, R: right leaflet.</p

(a)The leaflet shape shown in 3D (b)The leaflet shape shown “laid flat” in 2D.

<p>(a)The leaflet shape shown in 3D (b)The leaflet shape shown “laid flat” in 2D.</p

MicroRNA-532 and microRNA-3064 inhibit cell proliferation and invasion by acting as direct regulators of human telomerase reverse transcriptase in ovarian cancer

<div><p>Human telomerase reverse transcriptase (hTERT) plays a crucial role in ovarian cancer (OC) progression. However, the mechanisms underlying hTERT upregulation in OC, and the specific microRNAs (miRNAs) involved in the regulation of hTERT in OC cells, remains unclear. We performed a bioinformatics search to identify potential miRNAs that bind to the 3'-untranslated region (3'-UTR) region of the hTERT mRNA. We examined the expression levels of miR-532/miR-3064 in OC tissues and normal ovarian tissues, and analyzed the correlation between miRNA expression and OC patient outcomes. The impacts of miR-532/miR-3064 on hTERT expression were evaluated by western blot analysis and hTERT 3'-UTR reporter assays. We investigated the effects of miR-532/miR-3064 on proliferation and invasion in OC cells. We found that miR-532 and miR-3064 are down-regulated in OC specimens. We observed a significant association between reduced miR-532/miR-3064 expression and poorer survival of patients with OC. We confirmed that in OC cells, these two miRNAs downregulate hTERT levels by directly targeting its 3'-UTR region, and inhibited proliferation, EMT and invasion of OC cells. In addition, the overexpression of the hTERT cDNA lacking the 3'-UTR partially restored miR-532/miR-3064-inhibited OC cell proliferation and invasion. The silencing of hTERT by siRNA oligonucleotides abolished these malignant features, and phenocopied the effects of miR-532/miR-3064 overexpression. Furthermore, overexpression of miR-532/miR-3064 inhibits the growth of OC cells <i>in vivo</i>. Our findings demonstrate a miR-532/miR-3064-mediated mechanism responsible for hTERT upregulation in OC cells, and reveal a possibility of targeting miR-532/miR-3064 for future treatment of OC.</p></div

The width and height in millimeters of the pulmonary valve cusps.

<p>The width and height in millimeters of the pulmonary valve cusps.</p

Identification of hTERT as a target for miR-532 and miR-3064 in OC cells.

<p>(<b>A, B</b>) Luciferase assay was performed in OC cells that were co-transfected with reporter vectors carrying either the wild-type (WT) version or the mutated (MUT) version of hTERT 3'-UTR, along with either miR-532/miR-3064 mimics (<b>A</b>) or miR-532/miR-3064 inhibitors (<b>B</b>). Neg mimic: negative control miRNA mimic; Neg inhibitor: negative control miRNA inhibitor; (<b>C, D</b>) Expression of hTERT, Slug, E-cadherin, Bax and GAPDH was determined using western blotting in ES-2 (<b>C</b>) or SKOV-3 (<b>D</b>) cells transfected with miR-532/miR-3064 mimics or miR-532/miR-3064 inhibitors, respectively; (<b>E</b>) The qPCR analysis of hTERT levels in OC patient samples and normal ovarian tissue samples; (<b>F</b>) Kaplan-Meier survival curves showing lower overall survival in patients with high (above median value) versus low (below median value) hTERT levels. **: <i>P</i> < 0.01.</p