Effect of CaCl₂ on SOD, POD, and CAT activity on <i>Zoysia</i><i>japonica</i> leaves under drought.

<p>(A) SOD activity; (B) POD activity; (C) CAT activity. Vertical bars represent standard deviations (n=3). The different letters indicate significant difference among different treatments on a given day (p < 0.05, LSD multiple test). The asterisks indicate significant difference between given day and 0 d at the same treatment (p < 0.05, LSD multiple test).</p

Effect of CaCl₂ on net photosynthetic rate of <i>Zoysia</i><i>japonica</i> leaves under drought.

<p>Vertical bars represent standard deviations (n=3). The different letters indicate significant difference among different treatments on a given day (p < 0.05, LSD multiple test). The asterisks indicate significant difference between given day and 0 d at the same treatment (p < 0.05, LSD multiple test).</p

Effect of CaCl₂ on above- and below-ground biomass of <i>Zoysia</i><i>japonica</i> under drought.

<p>(A) Fresh weight; (B) Dry weight. Vertical bars represent standard deviations (n=3). The different letters indicate significant difference at p < 0.05 (LSD multiple test).</p

Soil water content during drought.

<p>Vertical bars represent standard deviations (n=3). The different letters indicate significant difference among different treatments on a given day (p < 0.05, LSD multiple test). The asterisks indicate significant difference between given day and 0 d at the same treatment (p < 0.05, LSD multiple test).</p

Effect of CaCl₂ on MDA and proline contents on <i>Zoysia</i><i>japonica</i> leaves under drought.

<p>(A) MDA content; (B) proline content. Vertical bars represent standard deviations (n=3). The different letters indicate significant difference among different treatments on a given day (p < 0.05, LSD multiple test). The asterisks indicate significant difference between given day and 0 d at the same treatment (p < 0.05, LSD multiple test).</p

Additional file 2 of The ontogenic gonadal transcriptomes provide insights into sex change in the ricefield eel Monopterus albus

Additional file 2: Table S1. Quality control parameters of the sequencing data. TableS2. Summary of high-quality reads mapped to the ribosome database. Table S3. Overview of mapping status for gonadal transcriptomes during sexual change of ricefield eel. Table S4. Number of reference genes and new genes in sequencing data. Table S5. Annotation of novel genes.Table S6. GO Enrichment Analysis of DEGs between EI vs F. Table S7. GO Enrichment Analysis of DEGs between MI vs EI. Table S8. GO Enrichment Analysis of DEGs between LI vs MI. Table S9. KEGG enrichment analysis of DEGs between EI vs F. Table S10. KEGG enrichment analysis of DEGs between MI vs EI. Table S11. KEGG enrichment analysis of DEGs between LI vs MI. Table S12. The expression of down-regulated DEGs for EI vs F during sex change. Table S13. The expression of up-regulated for DEGs for EI vs F during sex change

Effect of CaCl₂ on Fv<i>/</i>Fm of <i>Zoysia</i><i>japonica</i> leaves under drought.

<p>Vertical bars represent standard deviations (n=3). The different letters indicate significant difference among different treatments on a given day (p < 0.05, LSD multiple test). The asterisks indicate significant difference between given day and 0 d at the same treatment (p < 0.05, LSD multiple test).</p

New Application of Z‑Scheme Ag₃PO₄/g‑C₃N₄ Composite in Converting CO₂ to Fuel

This research was designed for the first time to investigate the activities of photocatalytic composite, Ag₃PO₄/g-C₃N₄, in converting CO₂ to fuels under simulated sunlight irradiation. The composite was synthesized using a simple <i>in situ</i> deposition method and characterized by various techniques including Brunauer–Emmett–Teller method (BET), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), UV–vis diffuse reflectance spectroscopy (DRS), photoluminescence spectroscopy (PL), and an electrochemical method. Thorough investigation indicated that the composite consisted of Ag₃PO₄, Ag, and g-C₃N₄. The introduction of Ag₃PO₄ on g-C₃N₄ promoted its light absorption performance. However, more significant was the formation of heterojunction structure between Ag₃PO₄ and g-C₃N₄, which efficiently promoted the separation of electron–hole pairs by a Z-scheme mechanism and ultimately enhanced the photocatalytic CO₂ reduction performance of the Ag₃PO₄/g-C₃N₄. The optimal Ag₃PO₄/g-C₃N₄ photocatalyst showed a CO₂ conversion rate of 57.5 μmol<b>·</b>h^–1<b>·</b>g_cat^–1, which was 6.1 and 10.4 times higher than those of g-C₃N₄ and P25, respectively, under simulated sunlight irradiation. The work found a new application of the photocatalyst, Ag₃PO₄/g-C₃N₄, in simultaneous environmental protection and energy production

Data_Sheet_10.PDF

<p>The neurohypophyseal hormone oxytocin (Oxt) has been shown to stimulate prolactin (Prl) synthesis and release from the adenohypophysis in rats. However, little is known about the functional roles of Oxt-like neuropeptides in the adenohypophysis of non-mammalian vertebrates. In this study, cDNAs encoding ricefield eel oxytocin-like receptors (Oxtlr), namely isotocin (Ist) receptor 1 (Istr1) and 2 (Istr2), were isolated and specific antisera were generated, respectively. RT-PCR and Western blot analysis detected the presence of both Istr1 and Istr2 in the brain and pituitary, but differential expression in some peripheral tissues, including the liver and kidney, where only Istr1 was detected. In the pituitary, immunoreactive Istr1 and Istr2 were differentially distributed, with the former mainly in adenohypophyseal cell layers adjacent to the neurohypophysis, whereas the latter in peripheral areas of the adenohypophysis. Double immunofluorescent images showed that immunostaining of Istr1, but not Istr2 was localized to growth hormone (Gh) cells, but neither of them was expressed in Prl cells. Ist inhibited Gh release in primary pituitary cells of ricefield eels and increased Gh contents in the pituitary gland of ricefield eels at 6 h after in vivo administration. Ist inhibition of Gh release is probably mediated by cAMP, PKC/DAG, and IP3/Ca²⁺ pathways. In contrast, Ist did not affect either prl gene expression or Prl contents in primary pituitary cells. Results of this study demonstrated that Ist may not be involved in the regulation of Prl, but inhibit Gh release via Istr1 rather than Istr2 in ricefield eels, and provided evidence for the direct regulation of Gh cells by oxytocin-like neuropeptides in the pituitary of non-mammalian vertebrates.</p

Data_Sheet_8.PDF

<p>The neurohypophyseal hormone oxytocin (Oxt) has been shown to stimulate prolactin (Prl) synthesis and release from the adenohypophysis in rats. However, little is known about the functional roles of Oxt-like neuropeptides in the adenohypophysis of non-mammalian vertebrates. In this study, cDNAs encoding ricefield eel oxytocin-like receptors (Oxtlr), namely isotocin (Ist) receptor 1 (Istr1) and 2 (Istr2), were isolated and specific antisera were generated, respectively. RT-PCR and Western blot analysis detected the presence of both Istr1 and Istr2 in the brain and pituitary, but differential expression in some peripheral tissues, including the liver and kidney, where only Istr1 was detected. In the pituitary, immunoreactive Istr1 and Istr2 were differentially distributed, with the former mainly in adenohypophyseal cell layers adjacent to the neurohypophysis, whereas the latter in peripheral areas of the adenohypophysis. Double immunofluorescent images showed that immunostaining of Istr1, but not Istr2 was localized to growth hormone (Gh) cells, but neither of them was expressed in Prl cells. Ist inhibited Gh release in primary pituitary cells of ricefield eels and increased Gh contents in the pituitary gland of ricefield eels at 6 h after in vivo administration. Ist inhibition of Gh release is probably mediated by cAMP, PKC/DAG, and IP3/Ca²⁺ pathways. In contrast, Ist did not affect either prl gene expression or Prl contents in primary pituitary cells. Results of this study demonstrated that Ist may not be involved in the regulation of Prl, but inhibit Gh release via Istr1 rather than Istr2 in ricefield eels, and provided evidence for the direct regulation of Gh cells by oxytocin-like neuropeptides in the pituitary of non-mammalian vertebrates.</p