MOESM6 of Genome-wide identification, expression profiles and regulatory network of MAPK cascade gene family in barley

Additional file 6: Figure S6. GO annotation of these identified barley MAPK cascade genes

Additional file 9 of CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Additional file 9

Additional file 8 of CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Additional file 8

Additional file 1 of CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Additional file 1

Image_1_Genetic Diversity of Transcription Factor Genes in Triticum and Mining for Promising Haplotypes for Beneficial Agronomic Traits.JPEG

Transcription factor (TF) is a class of the sequence-specific DNA-binding proteins that modulate the transcription of target genes, and thus regulate their expressions. Variations in TF are the crucial determinants for phenotypic traits. Although much progress has been made in the functions of TF genes in wheat, one of the most important staple crops globally, the diversity of TF genes in wheat and its progenitors are not well understood, especially the agronomically promising haplotypes have not yet been characterized. Here, we identified a total of 6,023 TF genes from hexaploid wheat through a genome-search method and classified them into 59 gene families based on the conserved domain. The characteristics and dN/dS values of these genes showed evidently selective effects. Based on re-sequencing data, we found a strong genetic bottleneck among these TF genes on A and D subgenomes while no found in B subgenome during wheat domestication. Combined with selective signals and known QTLs on the whole genome, 21 TF genes were preliminarily found to be associated with yield-related traits. The haplotype frequency of these TF genes was further investigated in bread wheat and its progenitors and 13 major haplotypes were the casual loci related to key traits. Finally, the tissue-specific TF genes were also identified using RNA-seq analysis. This study provided insights into the diversity and evolution of TF genes and the identified TF genes and excellent haplotypes associating with traits will contribute to wheat genetic improvement.</p

Additional file 2 of CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Additional file 2

MOESM5 of Genome-wide identification, expression profiles and regulatory network of MAPK cascade gene family in barley

Additional file 5: Figure S5. Evolutionary relationships and grouping among barley, rice and Arabidopsis MAPKs

Additional file 10 of CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Additional file 10

MOESM3 of Genome-wide identification, expression profiles and regulatory network of MAPK cascade gene family in barley

Additional file 3: Figure S3. Multiple sequence alignment of the HvMAPKK to identify the conserved kinase motifs. The red color marked are the signature motif of MAPKK proteins

Data_Sheet_1_Genetic Diversity of Transcription Factor Genes in Triticum and Mining for Promising Haplotypes for Beneficial Agronomic Traits.xlsx

Transcription factor (TF) is a class of the sequence-specific DNA-binding proteins that modulate the transcription of target genes, and thus regulate their expressions. Variations in TF are the crucial determinants for phenotypic traits. Although much progress has been made in the functions of TF genes in wheat, one of the most important staple crops globally, the diversity of TF genes in wheat and its progenitors are not well understood, especially the agronomically promising haplotypes have not yet been characterized. Here, we identified a total of 6,023 TF genes from hexaploid wheat through a genome-search method and classified them into 59 gene families based on the conserved domain. The characteristics and dN/dS values of these genes showed evidently selective effects. Based on re-sequencing data, we found a strong genetic bottleneck among these TF genes on A and D subgenomes while no found in B subgenome during wheat domestication. Combined with selective signals and known QTLs on the whole genome, 21 TF genes were preliminarily found to be associated with yield-related traits. The haplotype frequency of these TF genes was further investigated in bread wheat and its progenitors and 13 major haplotypes were the casual loci related to key traits. Finally, the tissue-specific TF genes were also identified using RNA-seq analysis. This study provided insights into the diversity and evolution of TF genes and the identified TF genes and excellent haplotypes associating with traits will contribute to wheat genetic improvement.</p