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<p>Cell scratch test and Transwell were used to measure the migration abilities of HSVSMCs. NC = Negative control group, only control siRNA transfected; GAS5(-) = lncRNA-GAS5 knockdown group transfected with silence siRNA. <b>A:</b>Cell scratch test was used to measure the migration abilities of HSVSMCs. The results showed that the HSVSMCs have the best migration abilities in the first 24 hours. Values are mean±SE, N = 4. <b>B:</b> The migration abilities of HSVSMCs measured by Transwell. After transfected by lncRNA-GAS5 siRNA for 48 hours, the HSVSMCs were passage into the Transwell Inserts. Then 4 hours, 7 hours, 10 hours later, the migration HSVSMCs were photographed and counted, respectively. Knockdown of lncRNA-GAS5 expression promotes migration of HSVSMCs. Optical microscope images under 200x magnification. <b>C:</b> The migration abilities of HSVSMCs were reflected indirectly by the new migration cells counting with Transwell. Silencing of lncRNA-GAS5 expression increses migration ability of HSVSMCs. Values are mean±SE, N = 10; *, P<0.05.</p

Subgroup analysis by ethnicity for the relationships between <i>CETP</i> rs708272 (C>T) polymorphism and myocardial infarction risk under the allele and dominant models.

<p>Subgroup analysis by ethnicity for the relationships between <i>CETP</i> rs708272 (C>T) polymorphism and myocardial infarction risk under the allele and dominant models.</p

Seven Functional Polymorphisms in the <i>CETP</i> Gene and Myocardial Infarction Risk: A Meta-Analysis and Meta-Regression

<div><p>Objective</p><p>This meta-analysis aims to evaluate the relationships between seven functional polymorphisms in the <i>CETP</i> gene and myocardial infarction (MI) risk.</p><p>Method</p><p>The PubMed, CISCOM, CINAHL, Web of Science, Google Scholar, EBSCO, Cochrane Library, and CBM databases were searched for relevant articles published before March 1st, 2013 without any language restrictions. Meta-analysis was conducted using the STATA 12.0 software.</p><p>Results</p><p>Nine case-control studies with a total 8,623 MI cases and 8,564 healthy subjects met the inclusion criteria. The results of our meta-analysis suggested that <i>CETP</i> rs708272 (C>T) polymorphism might be correlated with an increased risk of MI, especially among Caucasians. Furthermore, we observed that <i>CETP</i> rs1800775 (C>A) polymorphism might increase the risk of MI. Nevertheless, no similar findings were found for <i>CETP</i> rs5882 (A>G), rs2303790 (A>G), rs1800776 (C>A), rs12149545 (G>A), and rs4783961 (G>A) polymorphisms.</p><p>Conclusion</p><p>The current meta-analysis suggests that <i>CETP</i> rs708272 (C>T) and rs1800775 (C>A) polymorphisms may contribute to MI susceptibility, especially among Caucasians. Thus, <i>CETP</i> rs708272 and rs1800775 polymorphisms may be promising and potential biomarkers for early diagnosis of MI.</p></div

Univariate and multivariate meta-regression analyses of potential source of heterogeneity.

<p>SE = standard error, 95%CI = 95% confidence interval.</p

Meta-analysis of the associations between five common polymorphisms in <i>CETP</i> gene and MI risk.

<p>OR = odds ratios, 95%CI = 95% confidence interval, W = wild allele, M = mutant allele, WW = wild homozygote, WM = heterozygote, MM = mutant homozygote, SNP = single nucleotide polymorphism.</p

Forest plot of the relationships between <i>CETP</i> rs708272 (C>T) polymorphism and myocardial infarction risk under the allele and dominant models.

<p>Forest plot of the relationships between <i>CETP</i> rs708272 (C>T) polymorphism and myocardial infarction risk under the allele and dominant models.</p

Sensitivity analysis of the relationships of <i>CETP</i> rs708272 (C>T) and rs1800775 (C>A) polymorphisms with myocardial infarction risk.

<p>Sensitivity analysis of the relationships of <i>CETP</i> rs708272 (C>T) and rs1800775 (C>A) polymorphisms with myocardial infarction risk.</p

Main characteristics and methodological quality of all eligible studies.

<p>M = male, F = female, PB = population-based, HB = hospital-based, PCR = polymerase chain reaction, RFLP = restriction fragment length polymorphism, SNP = single nucleotide polymorphism, NOS = Newcastle-Ottawa quality assessment scale.</p

Meta-analysis of the associations between <i>CETP</i> rs708272 (G>A) and rs1800775 (C>A) polymorphisms and endometrial cancer risk.

<p>OR = odds ratios, 95%CI = 95% confidence interval, W = wild allele, M = mutant allele, WW = wild homozygote, WM = heterozygote, MM = mutant homozygote, PCR = polymerase chain reaction, RFLP = restriction fragment length polymorphism.</p

Flow chart shows study selection procedure.

<p>Flow chart shows study selection procedure.</p