Negotiation for Meaning Routines in Audio SCMC Interactions: An Expanded Framework

Negotiation for meaning, in response to instances of non-understanding, plays an important role in SLA. Meaning negotiation routines in face-to-face classroom interactions have been identified by Varonis and Gass. Smith expands the model to adapt it to text chat CMC environments. In the past decade, synchronous audio CMC has become commonly used for online language teaching, but its affordances are different from text chat CMC. Therefore, it is necessary to examine what meaning negotiation routines are in language learners’ oral interactions in this new online learning environment. In this study, participants were invited to complete two information gap tasks in which target lexical items were embedded to elicit learners’ negotiation for meaning and then they participated in a stimulated recall interview. Based on the analysis of students’ oral interactions in synchronous audio CMC, the authors propose two new possible stages in negotiation for meaning routines and demonstrate how different modes of communication can affect language learning online

Online Language Teacher Skills and Roles in an Audio-Graphic Conferencing Classroom

Many institutions and individual teachers are moving from traditional face-to-face classrooms to online teaching. Traditional classroom language teachers need to understand why online teaching is different from classroom teaching before they acquire new skills and explore new pedagogies for online teaching. This study aims to identify the differences between teaching online and in face-to-face classrooms, and explore what new skills and roles beginner online language teachers need to develop in order to become successful language teachers in online classrooms. Audio-graphic conferencing classrooms are usually a basic form of online teaching and the starting point for many face-to-face teachers to move to online teaching. This study collects data from an OU-Live EAP tutorial in the Open University UK. Four critical incidents were selected from an online tutorial and analysed through multimodal discourse analysis based on the Model of Instructor Roles by Berge (2005) and the Skills Pyramid by Hampel and Stickler (2005). A video-stimulated recall interview was conducted to elicit the online tutor’s rationale for his actions in the four critical incidents. The major findings of the study include: (a) three major differences between teaching online and in face-to-face classrooms, including technical differences, lack of non-verbal cues, and multimodality in online learning environments; (b) two suggestions for the Skills Pyramid on ‘dealing with the possibilities and constraints of the system’ and ‘online socialization skill’ (Hampel and Stickler, 2005); and (c) two suggestions for the Model of Instructor Roles the on pedagogical role and the technical role of online language teachers (Berge, 1995). Recommendations for online teacher training and future research topics are presented in the end

Negotiation For Meaning In Audio And Video Synchronous Computer-mediated Communication

After the outbreak of Covid-19 across the world, video conferencing tools have been widely used for online teaching all over the world. In synchronous computer-mediated communication (SCMC), where text, audio, video, and many other semiotic resources are simultaneously available, a huge challenge for interlocutors is how they can make good use of modes for their communication. Different modes of communication afforded by different types of technology can affect the way people communicate, and thus affecting the process of second language acquisition. Therefore, this study focuses on meaning negotiation episodes and aims to explore the following two research questions: (1) How do students negotiate meaning in audio SCMC and in video SCMC? (2) What roles do multiple modes and semiotic resources play in meaning negotiation episodes in video SCMC? Four dyads of Chinese postgraduate English language learners performed two types of lexically seeded information gap tasks in audio and video SCMC environments respectively. Meaning negotiation episodes were identified for data analysis. Video stimulated recall interviews were conducted to obtain participants' thoughts during meaning negotiation episodes. Three types of data analysis were carried out, including: (1) an interaction analysis of all audio SCMC negotiated interactions; (2) a statistical analysis of students’ gaze directions during meaning negotiation episodes in video SCMC; and (3) a multimodal analysis of students' verbal interactions, gaze directions, facial expressions, and gestures. The three types of in-depth analyses have led to the important findings. The study has proposed expanded meaning negotiation routines specifically for audio and video SCMC. The gaze analysis discovers a statistically significant positive relationship between the amount of time interlocutors spend looking at each other’s video images and the success of meaning negotiation. The multimodal analysis has revealed different levels of multimodal communicative competence and identified a range of relationships between different mode(s) in video SCMC

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Unravelling the room temperature growth of two-dimensional h-BN nanosheets for multifunctional applications

Room temperature growth of two-dimensional van der Waals (2D-vdW) materials is indispensable for state-of-the-art nanotechnology as it supersedes the requirement of elevated growth temperature accompanied with additional high thermal budgets, and the possibility of intrinsic interfacial thermal diffusion related deterioration of functional properties. Here, we demonstrated the growth of ultrawide-bandgap boron nitride (BN) at room temperature by using the pulsed laser deposition (PLD) process and demonstrated various functionalities for potential applications. Comprehensive chemical, spectroscopic and microscopic characterization confirms the growth of ordered nano-sheet-like hexagonal BN (h-BN). Functionally, nanosheets show hydrophobicity, high lubricity (low coefficient of friction), low refractive index within the visible to near-infrared wavelength range, and room temperature single-photon quantum emission. Our work unveils an important step that brings a plethora of applications ranging from the precise design of photonic devices, quantum information technology, transparent lubricant coatings, chemically inert capping layers for corrosive and ambient-air degradable materials, and 2D flexible nano-electronics, thus creating a scenario for “h-BN on demand” at frugal thermal budget

Unravelling the room temperature growth of two-dimensional h-BN nanosheets for multifunctional applications

Room temperature growth of two-dimensional van der Waals (2D-vdW) materials is indispensable for state-of-the-art nanotechnology as it supersedes the requirement of elevated growth temperature accompanied with additional high thermal budgets, and the possibility of intrinsic interfacial thermal diffusion related deterioration of functional properties. Here, we demonstrated the growth of ultrawide-bandgap boron nitride (BN) at room temperature by using the pulsed laser deposition (PLD) process and demonstrated various functionalities for potential applications. Comprehensive chemical, spectroscopic and microscopic characterization confirms the growth of ordered nano-sheet-like hexagonal BN (h-BN). Functionally, nanosheets show hydrophobicity, high lubricity (low coefficient of friction), low refractive index within the visible to near-infrared wavelength range, and room temperature single-photon quantum emission. Our work unveils an important step that brings a plethora of applications ranging from the precise design of photonic devices, quantum information technology, transparent lubricant coatings, chemically inert capping layers for corrosive and ambient-air degradable materials, and 2D flexible nano-electronics, thus creating a scenario for “h-BN on demand” at frugal thermal budget

Author Correction: Robust estimation of bacterial cell count from optical density

An amendment to this paper has been published and can be accessed via a link at the top of the paper.</jats:p

Robust estimation of bacterial cell count from optical density

AbstractOptical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals  &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.</jats:p